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Quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, Melanaphis sacchari

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Abstract

Yellow leaf caused by Sugarcane yellow leaf virus (SCYLV) is a serious constraint to sugarcane production in India and currently the disease epidemics occur on many of the susceptible varieties under field conditions. Studies were conducted on the virus transmission by sugarcane aphid Melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro- propagated plants of sugarcane cv Co 86032 with viruliferous aphids. Virus transmission was confirmed through RT-PCR assays and subsequently SCYLV population was established through RT-qPCR. A maximum of 22.3 × 103, 3.16 × 106 and 4.78 × 106 copies of SCYLV-RNA targets were recorded in the plants after 7, 180 and 300 days, respectively. This study showed that the aphid species M. sacchari acts as an effective vector of SCYLV. The relative standard curve method in RT-qPCR efficiently detected the increment in SCYLV copy numbers in sugarcane following transmission through M. sacchari.

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References

  1. Akbar W, Showler AT, Reagan TE, White WH. Categorizing sugarcane cultivar resistance to the sugarcane aphid and yellow sugarcane aphid (Hemiptera: Aphididae). J Econ Entomol. 2010;03:1431–7.

    Article  Google Scholar 

  2. Brault V, Herrbach E, Reinbold C. Electron microscopy studies on luteovirid transmission by aphids. Micron. 2007;38:302–12.

    Article  CAS  PubMed  Google Scholar 

  3. Chinnaraja C, Viswanathan R, Karuppaiah R, Bagyalakshmi K, Malathi P, Parameswari B. Complete genome characterization of Sugarcane yellow leaf virus from India: evidence for RNA recombination. Eur J Plant Pathol. 2013;135:335–49.

    Article  CAS  Google Scholar 

  4. Chinnaraja C, Viswanathan R, Sathyabhama M, Parameswari B, Bagyalakshmi K, Malathi P, Neelamathi D. Quantification of Sugarcane yellow leaf virus in in vitro plantlets and asymptomatic plants of sugarcane by RT-qPCR. Curr Sci. 2014;106:729–34.

    CAS  Google Scholar 

  5. Edon-Jock JC, Rott P, Vaillant J, Fernandez E, Girard JC, Daugrois JH. Status of Sugarcane yellow leaf virus in commercial fields and risk assessment in Guadeloupe. Proc Int Soc Sugar Cane Technol. 2007; 26:995–1004. Durban, South Africa: ISSCT.

  6. Francki RIB, Milne RG, Hatta T. Luteovirus group. In: Atlas of plant viruses, vol. I, Boca Raton: CRC Press; 1985. p. 137–141.

  7. Gray S, Gildow FE. Luteovirus aphids interaction. Annu Rev Phytopathol. 2003;41:539–99.

    Article  CAS  PubMed  Google Scholar 

  8. Izaguirre-Mayoral ML, Carballo O, Alceste C, Romano M, Nass HA. Physiological performance of asymptomatic and yellow leaf syndrome-affected sugarcanes in Venezuela. J Phytopathol. 2002;150:13–9.

    Article  CAS  Google Scholar 

  9. Lehrer AT, Schenck S, Yan SL, Komor E. Movement of aphid transmitted Sugarcane yellow leaf virus (SCYLV) within and between sugarcane plants. Plant Pathol. 2007;5(6):711–7.

    Article  Google Scholar 

  10. Lockhart BEL, Cronje CPR. Yellow leaf syndrome. In: Rott P, Bailey RA, Comstock JC, Croft BJ, Saumtally AS, editors. A guide to sugarcane diseases. Montpellier: La Librairie du Cirad; 2000. p. 291–5.

    Google Scholar 

  11. McAllister CD, Hoy JW, Reagan TE. Temporal increase and spatial distribution of the sugar cane yellow leaf and infestations of the aphid vector, Melanaphis sacchari. Plant Dis. 2008;92:607–15.

    Article  Google Scholar 

  12. Paray NB, Khoodoo MHR, Saumtally AS, Ganeshan S. Vector-virus relationship for Melanaphis sacchari (Zehnt.) (Hemiptera: aphididae) transmitting Sugarcane yellow leaf Luteovirus in Mauritius. Sugar Tech. 2011;13:77–80.

    Article  Google Scholar 

  13. Peiman M, Xie C. Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber. Australas Plant Dis Notes. 2006;1:41–6.

    Article  CAS  Google Scholar 

  14. Rassaby L, Girard JC, Lemaire O, Costet L, Irey MS, Kodja H, Lockhart BEL, Rott P. Spread of Sugarcane yellow leaf virus in sugarcane plants and fields on the island of Réunion. Plant Pathol. 2004;53:117–25.

    Article  Google Scholar 

  15. Rassaby L, Girard JC, Letourmy P, Chaume J, Irey MS, Lockhart BE. Impact of sugarcane yellow leaf virus on sugarcane yield and juice quality in Reunion Island. Eur J Plant Pathol. 2003;109:459–66.

    Article  Google Scholar 

  16. Schenck S. Yellow leaf syndrome-a new sugarcane disease. Hawaiian Sugar Planters Association: 1990; Annual report 38.

  17. Schenck S, Lehrer AT. Factors affecting the transmission and spread of Sugarcane yellow leaf virus. Plant Dis. 2000;84:1085–8.

    Article  Google Scholar 

  18. Singh D, Rao GP. Molecular detection of two strains of Sugarcane yellow leaf virus in India and their secondary spread in nature through aphids. Acta Phytopathol Entomol Hung. 2011;46:17–26.

    Article  CAS  Google Scholar 

  19. Vega J, Scagliusi SMM, Ulian EC. Sugarcane yellow leaf disease in Brazil: evidence of association with a luteovirus. Plant Dis. 1997;81:21–6.

    Article  Google Scholar 

  20. Viswanathan R. Sustainable ecofriendly disease management systems in sugarcane production under the changing climate—a review. J Mycol Plant Pathol. 2013;43:12–27.

    Google Scholar 

  21. Viswanathan R, Balamuralikrishnan M, Karuppaiah R. Identification of three genotypes of sugarcane yellow leaf virus causing yellow leaf disease from India and their molecular characterization. Virus Genes. 2008;37:368–79.

    Article  CAS  PubMed  Google Scholar 

  22. Viswanathan R, Rao GP. Disease scenario and management of major sugarcane diseases in India. Sugar Tech. 2011;13:336–53.

    Article  CAS  Google Scholar 

  23. Zhou G, Li J, Xu D, Shen W, Deng H. Occurrence of Sugarcane yellow leaf virus in South China and its transmission by the sugarcane-colonizing aphid. Ceratovacuna lanigera Sci Agric Sinica. 2006;39:2023–7.

    Google Scholar 

  24. Zhu YJ, Lim STS, Schenck S, Arcinas A, Komor E. RT-PCR and quantitative real-time RT-PCR detection of Sugarcane yellow leaf virus (SCYLV) in symptomatic and asymptomatic plants of Hawaiian sugarcane cultivars and the correlation of SCYLV titre to yield. Eur J Plant Pathol. 2010;127:263–73.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Dr. N. Vijayan Nair, the Director of the Institute for providing necessary facilities and support to conduct the work. The first author is thankful to the Department of Biotechnology, New Delhi for the support of Senior Research Fellow under NCS-TCP.

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Authors and Affiliations

  1. Plant Pathology Section, Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, 641007, India

    C. Chinnaraja & R. Viswanathan

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  1. C. Chinnaraja
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  2. R. Viswanathan
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Correspondence to R. Viswanathan.

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Chinnaraja, C., Viswanathan, R. Quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, Melanaphis sacchari . VirusDis. 26, 237–242 (2015). https://doi.org/10.1007/s13337-015-0267-7

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  • DOI: https://doi.org/10.1007/s13337-015-0267-7

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