Abstract
Symptoms like bright yellowing, puckering of the leaf, vein banding, and vein thickening were observed on different cucurbit hosts at the experimental farm of Indian Agricultural Research Institute, New Delhi during Kharif 2019. Leaf-dip electron microscopy of the symptomatic leaves revealed the association of isometric virus particles measuring ~ 25 nm with bitter gourd and cucumber samples. The RT-PCR assay using polerovirus generic primers covering the partial RdRp, intergenic region, and partial CP region was resulted the amplicons of ~ 1.1 kb. Subsequent cloning, sequencing, and sequence analysis revealed the association of cucurbit aphid-borne yellows virus (CABYV) with bitter gourd (Momordica charantia) and cucumber (Cucumis sativus) plants. These results constitute the first report of CABYV infection on cucumber plants from India.
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Funding was provided by the Indian Council of Agricultural Research (IN), New Delhi (India).
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Supplementary Figure 1.
Nucleotide sequence identity matrix generated based on partial RdRp, IR, and partial CP coding sequences of the bitter gourd and cucumber associated polerovirus of this study, in comparison with that of the other polerovirus isolates retrieved from the NCBI GenBank database. The isolates cloned and sequenced in the current study are marked with an asterisk. (PDF 208 kb)
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Kumar, A., Bhattarai, A., Rathore, A.S. et al. Association of cucurbit aphid-borne yellows virus with cucumber plants in India. VirusDis. 32, 183–185 (2021). https://doi.org/10.1007/s13337-020-00645-4
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DOI: https://doi.org/10.1007/s13337-020-00645-4