Skip to main content
SpringerLink
Log in

Incidence and characterization of Potato virus V infections in Iran

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

A survey was carried out to determine the extent of Potato virus V (PVV) infection, together with other potyviruses, in Iran in both commercial and local potato varieties. We found a low incidence of PVV in commercial varieties compared to a local potato cultivar Zardi, in which we noted a PVV infection up to ~32.9 %. We determined the genomic sequence 9,812 nucleotides of one isolate (KER.LAL.P) from cultivar Agria and the 3′-terminal sequence including coat protein (CP) gene of four additional isolates from cultivar Zardi. The Iranian isolate PVV KER.LAL.P was found to share 91 % sequence identity with the Scottish isolate DV-42 (AJ243766) of PVV. The CP gene sequences of the PVV isolates from Iran shared 96.5 to 99.3 % pairwise nucleotide identity and they shared <97.5 % pairwise identity with the CP sequences of all other PVV isolates available in public databases. Our host studies indicated that the Iranian PVV isolate had a narrower host range and infected Nicotiana debneyi and N. glutinosa test plants. Within the commercial varieties of potato in Iran, we noted a significant amount of mixed potyvirus infection. This study is the first report of occurrence and complete genome of PVV in Iran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Oruetxebarria I, Kekarainen T, Spetz C, Valkonen JPT. Molecular characterization of Potato virus V genomes from Europe indicates limited spatiotemporal strain differentiation. Phytopathology. 2000;90(4):437–44.

    Article  CAS  PubMed  Google Scholar 

  2. Jones RAC, Fribourg CE. Potato virus V. Descriptions of plant viruses No. 316. CMI/AAB. J Gen Virol. 1986;64:2471–7.

    Google Scholar 

  3. Oruetxebarria I, Valkonen JPT. Analysis of the P1 gene sequences and the 3′-terminal sequences and secondary structures of the single-stranded RNA genome of Potato virus v. Virus Genes. 2001;22(3):335–43.

    Article  CAS  PubMed  Google Scholar 

  4. Rozendaal A, van Binsbergen J, Anema B, van Slogteren DHM, Bunt MH. Serology of a deviating potato virus YC strain in the potato variety Gladblaadje. Potato Res. 1971;14:241.

    Google Scholar 

  5. Shiel PJ, Miller L, Slack SA, Berger PH. Isolation and partial nucleic acid characterization of a new isolate of Potato virus V with distinct biological and serological properties. Plant Dis. 2004;88(4):368–72.

    Article  CAS  Google Scholar 

  6. Spetz C, Taboada AM, Darwich S, Ramsell J, Salazar LF, Valkonen JPT. Molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in Peru. J Gen Virol. 2003;84:2565–78.

    Article  CAS  PubMed  Google Scholar 

  7. Oruetxebarria I, Guo DY, Merits A, Makinen K, Saarma M, Valkonen JPT. Identification of the genome-linked protein in virions of Potato virus A, with comparison to other members in genus Potyvirus. Virus Res. 2001;73(2):103–12.

    Article  CAS  PubMed  Google Scholar 

  8. Barker H. Extreme resistance to potato virus V in clones of Solanum tuberosum that are also resistant to potato viruses Y and A: evidence for a locus conferring broad-spectrum potyvirus resistance. Theor Appl Genet. 1997;95(8):1258–62.

    Article  CAS  Google Scholar 

  9. Valkonen JPT. Novel resistances to four potyviruses in tuber-bearing potato species, and temperature-sensitive expression of hypersensitive resistance to potato virus Y. Ann Appl Biol. 1997;130(1):91–104.

    Article  Google Scholar 

  10. Mortensen RJ, Shen XY, Reid A, Mulholland V. Characterization of viruses infecting potato plants from a single location in Shetland, an isolated Scottish Archipelago. J Phytopathol. 2010;158(9):633–40.

    CAS  Google Scholar 

  11. Sharifi M, Massumi H, Heydarnejad J, Pour AH, Shaabanian M, Rahimian H. Analysis of the biological and molecular variability of watermelon mosaic virus isolates from Iran. Virus Genes. 2008;37(3):304–13.

    Article  CAS  PubMed  Google Scholar 

  12. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Muhire B, Martin DP, Brown JK, Navas-Castillo J, Moriones E, Zerbini FM, et al. A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae). Arch Virol. 2013;. doi:10.1007/s00705-012-1601-7.

    PubMed  Google Scholar 

  14. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010;59(3):307–21.

    Article  CAS  PubMed  Google Scholar 

  15. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics. 2010;26(19):2462–3.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Johansen E, Rasmussen OF, Heide M, Borkhardt B. The complete nucleotide-sequence of pea seed-borne mosaic-virus RNA. J Gen Virol. 1991;72:2625–32.

    Article  CAS  PubMed  Google Scholar 

  17. Xiao XW, Frenkel MJ, Teakle DS, Ward CW, Shukla DD. Sequence diversity in the surface-exposed amino-terminal region of the coat proteins of seven strains of sugarcane mosaic virus correlates with their host range. Arch Virol. 1993;132(3–4):399–408.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research work was supported by the Iran National Science Foundation (INSF; Grant No. 87041270).

Author information

Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    Fatemeh Shamsadden-Saeed, Hossain Massumi, Sakineh Moradi, Mohammad Maddahian, Jahangir Heydarnejad & Akbar Hosseini Pour

  2. School of Biological Science, University of Canterbury, Christchurch, 8140, New Zealand

    Arvind Varsani

Authors
  1. Fatemeh Shamsadden-Saeed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hossain Massumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sakineh Moradi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Maddahian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jahangir Heydarnejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Akbar Hosseini Pour
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Arvind Varsani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hossain Massumi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 683 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shamsadden-Saeed, F., Massumi, H., Moradi, S. et al. Incidence and characterization of Potato virus V infections in Iran. VirusDis. 25, 78–84 (2014). https://doi.org/10.1007/s13337-013-0178-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-013-0178-4

Keywords

Search

Navigation