Abstract
Photoacoustic Doppler flow measurement based on continuous wave laser excitation owns the merit of clearly presenting the Doppler power spectra. Extending this technique to dual wavelengths can gain the spectral information of the flow sample extra to the flow speed information. An experimental system with two laser diodes respectively operated at 405 nm and 660 nm wavelengths is built and the flow measurement with black and red dyed polystyrene beads is performed. The measured Doppler power spectra can vividly reflect the flow speed, the flow direction, as well as the bead color. Since it is straightforward to further apply the same principle to multiple wavelengths, we can expect this type of spectroscopic photoacoustic Doppler flow measurement will be developed in the near future which will be very useful for studying the metabolism of the slowly moving red blood cell inside microvessels.
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References
L. V. Wang and L. Gao, “Photoacoutic microscopy and computed tomography: from bench to bedside,” Annual Review of Biomedical Engineering, 2014, 16: 155–185.
J. Weber, P. C. Beard, and S. E. Bohndiek, “Constrast agents for molecular photoacoustic imaging,” Nature Method, 2016, 13(8): 639–650.
A. B. E. Attia, G. B. Alasundaram, M. Moothanchery, U. S. Dinish, R. Z. Bi, V. Ntziachristos, et al., “A review of clinical photoacoustic imaging: current and future trends,” Photoacoustics, 2019, 16: 100144.
K. Maslov and L. V. Wang, “Photoacoustic imaging of biological tissue with intensity-modulated continuous-wave laser,” Journal of Biomedical Optics, 2008, 13(2): 024006.
G. Langer, B. Buchegger, J. Jacak, T. A. Klar, and T. Berer, “Frequency domain photoacoustic and fluorescence microscopy,” Biomedical Optics Express, 2016, 7(7): 2692–2702.
P. LeBoulluec, H. L. Liu, and B. H. Yuan, “A cost-efficient frequency-domain photoacoustic imaging system,” American Journal of Physics, 2013, 81(9): 712–717.
O. Hugon, B. V. D. Sanden, M. Inglebert, O. Jacquin, C. Misbath, and E. Lacot, “Multi-wavelength photo-acoustic microscopy in the frequency domain for simultaneous excitation and detection of dyes,” Biomedical Optics Express, 2019, 10(2): 932–943.
P. Mohajerani, S. Kellnberger, and V. Ntziachristos, “Frequency domain optoacoustic tomography using amplitude and phase,” Photoacoustic, 2014, 2(3): 111–118.
S. Kellnberger, D. Soliman, G. J. Tserevelakis, M. Seeger, H. Yang, A. Karlas, et al., “Optoacoustic microscopy at multiple discrete frequencies,” Light: Science & Applications, 2018, 7(1): 1–12.
S. Y. Liu, K. Tang, X. H. Feng, H. R. Jin, F. Gao, and Y. J. Zheng, “Toward wearable healthcare: a miniaturized 3D imager with coherent frequency-domain photoacoustics,” IEEE Transactions on Biomedical Circuits and Systems, 2019, 13(6): 1417–1424.
S. S. S. Choi and A. Mandelis, “Review of the state of the art in cardiovascular endoscopy imaging of atherosclerosis using photoacoustic techniques with pulsed and continuous-wave optical excitations,” Journal of Biomedical Optics, 2019, 24(8): 080902.
H. Fang, K. Maslov, and L. V. Wang, “Photoacoustic Doppler effect from flowing small light-absorbing particles,” Physical Review Letters, 2007, 99(18): 184501.
H. Fang, K. Maslov, and L. V. Wang, “Photoacoustic Doppler flow measurement in optically scattering media,” Applied Physics Letters, 2007, 91(26): 264103.
H. Fang and L. V. Wang, Photoaoustic Doppler effect and flow sensing. Photoacoustic Imaging and Spectroscopy, L. V. Wang, Ed. Boca Raton, FL: CRC Press, 2009: 19–24.
A. Sheinfeld, S. Gilead, and A. Eyal, “Photoacoustic Doppler measurement of flow using tone bust excitation,” Optics Express, 2010, 18(5): 4212–4221.
A. Sheinfeld, S. Gilead, and A. Eyal, “Simultaneous spatial and spectral mapping of flow using photoacoustic Doppler measurement,” Journal of Biomedical Optics, 2010, 15(6): 066010.
Y. Tong, H. C. Zhao, H. Fang, Y. Q. Zhao, and X. C. Yuan, “Flow angle dependent photoacoustic Doppler power spectra under intensity-modulated continuous wave laser excitation,” AIP Advances, 2016, 6(2): 025109.
P. J. Van den Berg, K. Daoudi, and W. Steenbergen, “Review of photoacoustic flow imaging: its current state and its promises,” Photoacoustics, 2015, 3(3): 89–99.
L. D. Wang, K. Maslov, and L. V. Wang, “Single-cell label-free photoacoustic flowoxigraphy in vivo,” Proceedings of the National Academy of Sciences, 2013, 110(15): 5759–5764.
F. Yang, W. Song, C. L. Zhang, C. J. Min, H. Fang, L. P. Du, et al., “Broadband graphene-based photoacousitc microscopy with high sensitivity,” Nanoscale, 2018, 10(18): 8606–8614.
Acknowledgment
This work is supported by the National Natural Science Foundation of China (Grant No. 11774256) and the Natural Science Foundation of Guangdong Province (Grant No. 2018B03031104).
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Zhao, H., Liu, Y., Farooq, T. et al. Dual-Wavelength Continuous Wave Photoacoustic Doppler Flow Measurement. Photonic Sens 12, 1–9 (2022). https://doi.org/10.1007/s13320-021-0633-6
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DOI: https://doi.org/10.1007/s13320-021-0633-6