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Xanthomonas euvesicatoria pv. perforans is the causative agent of bacterial leaf spot on Ipomoea aquatica from Fujian Province in China

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Abstract

Identifying the causative agent of a disease provides key information for diagnosis and management. Here, we identified the causative agent of a leaf spot disease prevalent in water spinach (Ipomoea aquatica) in Fujian Province, China. Ninety bacterial isolates were recovered from symptomatic leaves of water spinach collected across six growing areas in Fujian Province. Completion of Koch’s postulates confirmed that 60 yellow mucoid isolates were the causative agents of the disease. PCR amplification of 16S ribosomal RNA gene sequences demonstrated that the isolates belonged to the genus Xanthomonas. We then PCR-amplified and sequenced the recA, dnaK, gyrB, and rpoD genes of the isolated pathogens for multilocus sequence analysis. In the resulting phylogenetic tree, the isolated pathogens grouped with X. euvesicatoria pv. perforans strain TC2-1. Biological and chemical tests performed on 60 isolates showed that all of them were positive in hydrogen sulfide, litmus milk, starch hydrolysis and bile-esculin tests, but negative in nitrate reduction, pectinase activity, and oxidase activity tests. The isolates could also cause disease symptoms on tomato (Solanum lycopersicum) leaves and stems but not on pepper (Capsicum annuum) plants. Based on DNA characteristics, physiological and chemical properties, and pathogenicity test results, we propose that the leaf spot disease in water spinach is caused by X. euvesicatoria pv. perforans.

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Acknowledgment

We thank Dr. Jianqiang Li and Dr. Laixin Luo from the Seed Health Centre, China Agricultural University for kindly providing the reference strain X. euvesicatoria pv. perforans XJ1602. We thank Dr. Guokun Liu from Fujian Agriculture and Forestry University, Fuzhou, China for kindly providing technical assistance.

Funding

This work was supported by the National Natural Science Foundation of China (31801696), Fund for Basic Research Projects of Fujian Academy of Agricultural Sciences (CXTD2021004-3, XTCXGC2021011, XTCXGC2021017).

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Authors and Affiliations

  1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, China

    Xiaojing Fan & Yong Chen

  2. Fujian Agriculture and Forestry University, Fuzhou, China

    Xiaojing Fan, Hanwei Zheng, Haiyan Luo & Tao Zhuo

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  1. Xiaojing Fan
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  2. Hanwei Zheng
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  3. Haiyan Luo
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Correspondence to Yong Chen.

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Fan, X., Zheng, H., Luo, H. et al. Xanthomonas euvesicatoria pv. perforans is the causative agent of bacterial leaf spot on Ipomoea aquatica from Fujian Province in China. Australasian Plant Pathol. 52, 327–337 (2023). https://doi.org/10.1007/s13313-023-00924-w

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