Abstract
Verticillium dahliae is an agricultural phytopathogen of notable importance around the world and is increasingly impacting the billion-dollar Australian cotton industry. In diseased Australian cotton, the VCG classification system of V. dahliae does not adequately reflect the disease symptoms observed. The non-defoliating VCG2A isolates have caused significant damage, a problem not readily observed overseas, and the defoliating VCG1A isolates have not always caused widespread severe symptoms as expected. This study examined a selection of V. dahliae isolates derived from diseased cotton plants in NSW, Australia, America and Israel and classified them into groups using Inter-Simple Sequence Repeats (ISSR). A subset of these isolates were tested in a pathogenicity assay using cotton plants. The combined results confirmed that Australia has VCG2A isolates capable of causing significant disease (“defoliating-like”) and that when analysed using ISSR, they are distinct from the non-virulent VCG2A populations. Additionally, there is a group of VCG1A isolates that are able to cause significant damage to cotton plants. The ISSR analysis provides a better understanding of the V. dahliae populations circulating within Australia and is a useful tool for diagnostic use, with a potential use for diagnostic confirmation of a causative strain.
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Acknowledgements
This project is supported by funding from the Australian Government Department of Agriculture as part of its Rural R&D for Profit programme, the Cotton Research and Development Corporation, and AUSGEM. Thank you to Dr Terry Wheeler for supplying three V. dahliae isolates, Dr Libo Shan from Texas A&M University for allowing the use of her laboratory facilities for DNA extractions, and to Leah Tsror (Lahkim), Dept of Plant Pathology and Weed Research, ARO, Volcani Center, Gilat Research Center, for supplying DNA of six isolates from Israel. John Webster and Bernie Dominiak reviewed and improved an earlier version of this manuscript.
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Dadd-Daigle, P., Collins, D., Kirkby, K. et al. Characterisation of Verticillium dahliae by inter-simple sequence repeats identified a virulent subpopulation affecting Australian cotton. Australasian Plant Pathol. 51, 409–418 (2022). https://doi.org/10.1007/s13313-022-00865-w
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DOI: https://doi.org/10.1007/s13313-022-00865-w