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Molecular marker aided characterization of race specific and non-race specific rust resistance genes in elite wheat (Triticum spp.) germplasm

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Abstract

Among wheat diseases, leaf (brown) rust caused by Puccinia triticina Eriks. causes more damage to the crop than any other rust. The pathogen is very dynamic and renders rust-resistant wheat cultivars susceptible by evolving new pathotypes. To counter such threats, developing wheat cultivars using diverse germplasm carrying rust resistance genes effective at different growth stages is required. Forty elite wheat genotypes were selected for characterizing race specific (Lr19/Sr25, Lr24/Sr24) and non-race specific (Lr34/Yr18/Pm38/Sr57, Lr46/Yr29/Pm39) rust resistance genes using Sequence Tagged Site (STS) markers Sr24#12, Gb, csLV34 and SSR marker wmc44. The marker analysis revealed the presence of Lr24/Sr24 genes in 47% of the wheat genotypes, whereas 22.2% possessed Lr24/Sr24 and Lr34/Yr18/Pm38/Sr57 gene combination. Two genotypes G16 and G12 were confirmed to have Lr19/Sr25, Lr24/Sr24, Lr34/Yr18/Pm38/Sr57 and Lr24/Sr24, Lr34/Yr18/Pm38/Sr57, Lr46/Yr29/Pm39 gene combinations, respectively. Genetic diversity analysis based on the dissimilarity indices indicated the presence of huge genetic diversity among the wheat germplasm. The presence of Lr24/Sr24 using Sr24#12 marker was confirmed in HS545, which showed monogenic control of leaf rust resistance against pathotype 77–5 (THTTM). Leaf rust and stem rust resistance present in this set of diverse germplasm could be used as potent donors for durable rust resistance breeding in wheat. The molecular markers utilized in this study would also be useful in pyramiding or transferring the R genes into the rust susceptible agronomically superior wheat germplasm. Genotypes G12 and G19 resistant to rusts and also had significant grain yield superiority over HS490 could be a better choice for their use in rust resistance breeding.

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Acknowledgements

The authors are grateful to the Director, ICAR- IARI, New Delhi for generous support for conducting this study.

Funding

Indian Council of Agriculture Research (ICAR), New Delhi.

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Authors and Affiliations

  1. ICAR-Indian Agricultural Research Institute, Regional Station [CHC], Shimla, 171004, Himachal Pradesh, India

    Dharam Pal & Madhu Patial

  2. ICAR-Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Shimla, 171002, Himachal Pradesh, India

    Subodh Kumar & S. C. Bhardwaj

  3. ICAR- Indian Agricultural Research Institute, New Delhi, 110012, India

    Harikrishna, Narayana Bhat Devate & Sunil Kumar V. Parmeshwarappa

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  1. Dharam Pal
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  4. Harikrishna
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Contributions

DP conceived the research idea; SCB did the host pathogen interaction tests; SK, Harikrishna and SKVP did molecular work; NBD carryout diversity analysis; MP helped in data recording; DP and SCB wrote the manuscript.

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Correspondence to Dharam Pal.

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13313_2022_850_MOESM2_ESM.pptx

Supplementary file2 (PPTX 23218 KB) Fig.S1. Lane M-100bp ladder, 1-36:genotypes; G16, G19, G22, G36 showing amplification of Gb marker for Sr25/Lr19;Lr19 (+ check); AL (– check). Fig. S2. Lane M-100bp ladder, 1-36: genotypes; G1, G2, G3,G4, G5, G6, G8, G9, G10, G11, G12, G16, G17, G20, G21, G24, G34 showingamplification of Sr24#12 marker for Sr24/Lr24; Lr24(+check); AL (– check). Fig. S3. Lane M-100bp ladder, 1-36: genotypes; G1, G3, G4, G6, G7, G8, G9, G10, G11,G12, G16, G32, G33, G34 showing amplification of marker csLV34 for Lr34;Lr34 (+ check); AL (– check). Fig.S4. Lane M-100bp ladder,1-36: genotypes; G2, G10, G12, G15, G19, G21, G29, G30, G31 showingamplification of marker wmc44-1BL for Lr46/Yr29; Lr46(+ check); AL (– check)

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Pal, D., Kumar, S., Bhardwaj, S.C. et al. Molecular marker aided characterization of race specific and non-race specific rust resistance genes in elite wheat (Triticum spp.) germplasm. Australasian Plant Pathol. 51, 261–272 (2022). https://doi.org/10.1007/s13313-022-00850-3

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