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Fusarium oxysporum f. sp. passiflorae infecting passionfruit in New Zealand in a changing taxonomic landscape

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Abstract

Fusarium oxysporum f. sp. passiflorae (FOP) is reported for the first time in Northland, New Zealand. The identity of this host-specific pathogen was confirmed by pathogenicity testing, morphological characters, and DNA sequencing. Pathogenic strains of Fusarium oxysporum secrete unique proteins or effectors, ‘secreted in xylem’ (SIX), which are likely to contribute to host-specific virulence. Sequence analysis of the EF-1a gene, β-tubulin and the effector genes SIX6 and SIX9 confirmed that New Zealand isolates belong to FOP. This study confirmed that the three New Zealand EF-1α haplotypes of FOP had identical SIX6 and SIX9 sequences, indicating that the same homolog of each gene, SIX6a and SIX9a, is shared by both haplotypes of FOP. SIX genes are rarely detected in non-pathogenic strains of Fusarium oxysporum species complex (FOSC) and pathogenicity tests are necessary to confirm its pathogenicity status.

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Acknowledgements

The authors would like to thank Dr Merje Toome for reviewing this manuscript, Emma Scheltema for figure layout, and all the Mycology & Bacteriology team who assisted with the study.

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Authors and Affiliations

  1. Ministry for Primary Industries, Plant Health and Environment Laboratory, 231 Morrin Rd, St Johns, Auckland, 1072, New Zealand

    R. Thangavel, J. A. Pattemore, K. B. Rebijith & B. J. R. Alexander

  2. AsureQuality, Lincoln University, South Drive, Lincoln, 7647, New Zealand

    N. Grbavac, S. Ganev & N. Chan

  3. Ministry for Primary Industries, Biosecurity Surveillance and Incursion Investigation Team, 66 Ward Street Wallaceville, Wellington, 5018, New Zealand

    H. G. Pearson

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  1. R. Thangavel
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Correspondence to R. Thangavel.

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Thangavel, R., Pattemore, J.A., Rebijith, K.B. et al. Fusarium oxysporum f. sp. passiflorae infecting passionfruit in New Zealand in a changing taxonomic landscape. Australasian Plant Pathol. 50, 365–377 (2021). https://doi.org/10.1007/s13313-021-00782-4

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