Skip to main content
SpringerLink
Log in

Fusarium oxysporum f. sp. lavandulae, a novel forma specialis causing wilt on Lavandula × allardii

  • Original Article
  • Published:
Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Symptoms of a vascular wilt were observed on many plants of Lavandula × allardii (hybrid of Lavandula dentata and L. latifolia) cultivated in a nursery near Albenga, Liguria region, Northern Italy. After identification based on morphological characteristic and ITS sequence analysis, the pathogen was identified as Fusarium oxysporum. Eight single-spore isolates obtained from infected tissues were used for a phylogenetic analysis in order to identify the forma specialis. Translation elongation factor 1-α (EF-1α), intergenic spacer (IGS) and three genes encoding for polygalacturonase genes (pg1, pg5 and pgx1) were amplified by PCR. Gene sequences were aligned with other formae speciales of Fusarium oxysporum obtained from GenBank databases in order to build phylogenetic trees. Results obtained for each genomic region showed a unique group with isolates derived from L. × allardii well separated from the other formae speciales. Results obtained, together with pathogenicity tests, allowed us to introduce a new forma specialis named F. oxysporum f. sp. lavandulae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Appel DJ, Gordon TR (1995) Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Exp Mycol 19:120–128

    Article  CAS  PubMed  Google Scholar 

  • Armstrong GM, Armstrong JK (1981) Formae speciales and races of Fusarium oxysporum causing wilt diseases. In: Nelson PE, Toussoun TA, Cook RJ (eds) Fusarium: diseases, biology and taxonomy. Penn State University Press, University Park, pp 391–399

    Google Scholar 

  • Baayen RP, O’Donnell K, Bonants PJM, Cigelnik E, Kroon LPNM, Roebroeck JA, Waalwijk C (2000) Gene genealogies and AFLP analysis in the Fusarium oxysporum complex identify monophyletic and non-monophyletic formae speciales causing wilt and rot disease. Phytopathology 90:891–900

    Article  CAS  PubMed  Google Scholar 

  • Cavanagh HMA, Wilkinson JM (2002) Biological activities of lavender essential oil. Phytother Res 16:301–308

    Article  CAS  PubMed  Google Scholar 

  • Cosic J, Vrandecic K, Jurkovic D, Postic J (2012) First report of lavender wilt caused by Fusarium sporotrichioides in Croatia. Plant Dis 96:591

    Article  PubMed  Google Scholar 

  • Daboussi MJ, Capy P (2003) Transposable elements in filamentous fungi. Annu Rev Microbiol 57:275–299

    Article  CAS  PubMed  Google Scholar 

  • Davino S, Cacciola SO, Pennisi AM, Li Destri Nicosia MG (2002) Phytophthora palmivora a new pathogen of lavender in Italy. Plant Dis 86:561

    Article  PubMed  Google Scholar 

  • Faedda R, Cacciola SO, Pane A, Szigethy A, Bakonyi J, Man in't Veld WA, Martini P, Schena L, Magnano di San Lio G (2013) Phytophthora × pelgrandis causes root and collar rot of Lavandula stoechas in Italy. Plant Dis 97:1091–1096

    Article  CAS  PubMed  Google Scholar 

  • Fisher NL, Burgess LW, Toussoun TA, Nelson PE (1982) Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72:151–153

    Article  Google Scholar 

  • Gámez MJ, Jiménez J, Risco S, Zarzuelo A (1987) Hypoglycemic activity in various species of the genus Lavandula. Part I: Lavandula stoechas L. and Lavandula multifida L. Pharmazie 42:706–707

    PubMed  Google Scholar 

  • Garibaldi A, Bertetti D, Pensa P, Ortu G, Gullino ML (2015) First report of Fusarium oxysporum causing wilt on Allard's lavender (Lavandula × allardii) in Italy. Plant Dis 99:1868

    Article  Google Scholar 

  • Gordon TR, Martyn RD (1997) The evolutionary biology of Fusarium oxysporum. Annu Rev Phytopathol 35:111–128

    Article  CAS  PubMed  Google Scholar 

  • Gullino ML, Katan J, Garibaldi A (2012) The genus Fusarium and the species that affect greenhouse vegetables and ornamentals. In: Gullino ML, Katan J, Garibaldi A (eds) Fusarium wilts of greenhouse vegetable and ornamental crops. APS Press, St. Paul, pp 5–9

    Google Scholar 

  • Hirano Y, Arie T (2009) Variation and phylogeny of Fusarium oxysporum isolates based on nucleotide sequences of polygalacturonase genes. Microbes Environ 24:113–120

    Article  PubMed  Google Scholar 

  • Leslie JF (2012) Genetics and Fusarium oxysporum. In: Gullino ML, Katan J, Garibaldi A (eds) Fusarium wilts of greenhouse vegetable and ornamental crops. APS Press, St. Paul, pp 39–47

    Google Scholar 

  • Lievens B, Hanssen IM, Rep M (2012) Recent developments in the detection and identification of formae speciales and races of Fusarium oxysporum: from pathogenicity testing to molecular diagnostics. In: Gullino ML, Katan J, Garibaldi A (eds) Fusarium wilts of greenhouse vegetable and ornamental crops. APS Press, St. Paul, pp 47–55

    Google Scholar 

  • Mbofung GY, Hong SG, Pryor BM (2007) Phylogeny of Fusarium oxysporum f. Sp. lactucae inferred from mitochondrial small subunit, elongation factor 1-α and nuclear ribosomal intergenic spacer sequence data. Phytopathology 97:87–98

    Article  CAS  PubMed  Google Scholar 

  • Miller AG (1985) The genus Lavandula in Arabia and tropical NE Africa. Notes Roy Bot Gard Edinburgh 42:503–528

    Google Scholar 

  • O’Donnell K, Cigelnik E (1999) A DNA sequence-based phylogenetic structure for the Fusarium oxysporum complex. Phytoparasitica 27:69–70

    Google Scholar 

  • O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci U S A 95:2044–2049

    Article  PubMed  PubMed Central  Google Scholar 

  • O’Donnell K, Gueidan C, Sink S, Johnston PR, Crous PW, Glenn A, Riley R, Zitomer NC, Colyer P, Waalwijk C, van der Lee T, Moretti A, Kang S, Kim H-S, Geiser DM, Juba JH, Baayen RP, Cromey MG, Bithell S, Sutton DA, Skovgaard KR, Kistler P, Elliott HC, Davis M, Sarver MBAJ (2009) A two locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genet Biol 46:936–948

    Article  PubMed  CAS  Google Scholar 

  • Ortu G, Bertetti D, Gullino ML, Garibaldi A (2013) A new forma specialis of Fusarium oxysporum on Crassula ovata. J Plant Pathol 95:33–39

    Google Scholar 

  • Ortu G, Bertetti D, Gullino ML, Garibaldi A (2015a) Fusarium oxysporum f. Sp. echeveriae, a novel forma specialis causing crown and stem rot of Echeveria agavoides. Phytopathol Mediterr 54:64–75

    CAS  Google Scholar 

  • Ortu G, Bertetti D, Martini P, Gullino ML, Garibaldi A (2015b) Fusarium oxysporum f. Sp. papaveris: a new forma specialis isolated from Iceland poppy (Papaver nudicaule). Phytopathol Mediterr 54:76–85

    CAS  Google Scholar 

  • Perveen K, Bokhari N (2010) First report of Fusarium wilt of Lavandula pubescens caused by Fusarium oxysporum in Saudi Arabia. Plant Dis 94:1163

    Article  PubMed  Google Scholar 

  • Putnam M (1991) Root rot of lavender caused by Phytophthora nicotianae. Plant Pathol 40:480–482

    Article  Google Scholar 

  • Recorbet G, Steinberg C, Olivain C, Edel V, Trouvelot S, Dumas-Gaudot E, Gianinazzi S, Alabouvette C (2003) Wanted: pathogenesis-related marker molecules for Fusarium oxysporum. New Phytol 159:73–92

    Article  CAS  PubMed  Google Scholar 

  • Ren YZ, Tan H, Li ZJ, Du J, Li H (2008) First report of lavender wilt caused by Fusarium solani in China. Plant Pathol 57:377–377

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Van der Does HC, Rep M (2007) Virulence genes and the evolution of host specificity in plant-pathogenic fungi. Mol Plant-Microbe Interact 20:1175–1182

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 261752, PLANTFOODSEC “Plant and Food Biosecurity, Network of Excellence”.

Author information

Authors and Affiliations

  1. Agroinnova – Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco, Turin, Italy

    Giuseppe Ortu, Domenico Bertetti, Maria Lodovica Gullino & Angelo Garibaldi

  2. DISAFA - Dipartimento di Scienze Agrarie, Forestali e Alimentari, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco, Turin, Italy

    Maria Lodovica Gullino

Authors
  1. Giuseppe Ortu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Domenico Bertetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria Lodovica Gullino
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Angelo Garibaldi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Giuseppe Ortu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ortu, G., Bertetti, D., Gullino, M.L. et al. Fusarium oxysporum f. sp. lavandulae, a novel forma specialis causing wilt on Lavandula × allardii. J Plant Pathol 100, 391–397 (2018). https://doi.org/10.1007/s42161-018-0084-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42161-018-0084-0

Keywords

Search

Navigation