Abstract
The aim of this work was to characterize the secondary metabolite (SMs) profile of Stemphylium lycopersici, a necrotrophic fungus that causes the disease known as grey leaf spot. We detected twenty-four SMs in cultures of S. lycopercisi within the extracts of 19 isolates. Each of them presented a characteristic unique profile. While highly virulent isolates synthesized a high number of metabolites (15), the low virulent ones synthesized a low number of SMs (2). However, a PCA analysis could not establish the relationship between SMs and virulence. Infectopyrone was the most frequent metabolite produced by the isolates (89%).
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Acknowledgements
We wish to thank Dr. Kristian Nielsen from Department of Biotechnology and Biomedicine (Technical University of Denmark, Lyngby, Denmark) for his assistance in performing the UHPLC–HRMS analyses and the critical reading of the manuscript. RM, AP and MCNS are members of the Research Career of CONICET, Argentina. PAB is a researcher from CICPBA, Argentina.
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Rocio Medina: Conceptualization, formal analysis, investigation, visualization, writing - original draft. Mario E. E. Franco: Conceptualization, investigation, visualization, writing - review and editing. Lucía da Cruz Cabral: Conceptualization, data curation, methodology. José Vera Bahima: methodology; Andrea Patriarca: Data curation, formal analysis, methodology, investigation, project administration, resources, software, supervision; validation; visualization, writing - review and editing. Pedro A. Balatti: Conceptualization, formal analysis, investigation, project administration, resources, visualization, writing – review and editing. Mario C. N. Saparrat: Conceptualization, investigation, methodology, project administration, resources, visualization, writing - review and editing.
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Medina, R., Franco, M.E.E., da Cruz Cabral, L. et al. The secondary metabolites profile of Stemphylium lycopersici, the causal agent of tomato grey leaf spot, is complex and includes host and non-host specific toxins. Australasian Plant Pathol. 50, 105–115 (2021). https://doi.org/10.1007/s13313-020-00753-1
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DOI: https://doi.org/10.1007/s13313-020-00753-1