Skip to main content
SpringerLink
Log in

Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy

  • Research Note
  • Published:
Australasian Plant Pathology Aims and scope Submit manuscript

Abstract

Six in vitro pear cultivars, Wonhwang, Xuehua, Conference, Stankimson, Starcrimson and Red Bastlett were infected by Apple stem pitting virus (ASPV) and were treated by combination of 25 μg/ml ribavirin and temperature 35 °C. Results showed that ribavirin could enhance the proliferation of Xuehua, Conference, Stankimson and Starcrimson and death plants were found in Xuehua and Red Bastlett and the total survival rate of the six cultivars was 93.5%. After treatment, the regenerated plants were detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that ASPV in all regenerated plants could not be detected and the total elimination rate of the six cultivars was 92.3%. The combination showed high efficacy on elimination of ASPV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Astruc N, Marcos JF, Macquaire G, Candresse T, Pallfis V (1996) Studies on the diagnosis of Hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents. Eur J Plant Pathol 102:837–846

    Article  CAS  Google Scholar 

  • Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L, Zurcher EJ (1996) Apple stem pitting virus. Plant viruses online: descriptions and lists from the VIDE database. CAB International, London

    Google Scholar 

  • Cieślińska M (2007) Application of thermo- and chemotherapy In vitro for eliminating some viruses infecting Prunus sp. fruit trees. J Fruit Ornam Plant Res 15:117–124

    Google Scholar 

  • Cieślińska M, Zawadzka B (1999) Preliminary results of investigation on elimination of viruses from apple, pear and raspberry using thermotherapy and chemotherapy in vitro. Phytopatholgy 17:41–48

    Google Scholar 

  • Desvignes JC, Boyé R (1988) Different diseases caused by the Chlorotic leaf spot virus on the fruit trees. Acta Hortic 235:31–38

    Google Scholar 

  • Hauptmanová A, Polák J (2011) The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures. Hort Sci (Prague) 38:49–53

    Article  Google Scholar 

  • Hu GJ, Hong N, Wang LP, Hu HJ, Wang GP (2012) Efficacy of virus elimination from in vitro cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy. Crop Prot 37:20–25

    Article  CAS  Google Scholar 

  • Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F, Zhou J (2015) Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell Tissue Organ Cult 121:435–443

    Article  CAS  Google Scholar 

  • Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren FX, Li ZN, Zhang SN (2018) Elimination of grapevine rupestris stem pitting-associated virus from vitis vinifera ‘kyoho’ by an antiviral agent combined with shoot tip culture. Sci Hortic 229:99–106

    Article  CAS  Google Scholar 

  • Jelkmann W, Paunovic S (2011) Apple stem pitting virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 35–40

    Chapter  Google Scholar 

  • Koubouris G, Maliogka V, Efthimiou K, Katis N, Vasilakakis M (2007) Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou. J Gen Plant Pathol 73:370–373

    Article  Google Scholar 

  • Lerch B (1987) On the inhibition of plant virus multiplication by ribavirin. Antivir Res 7:257–270

    Article  PubMed  CAS  Google Scholar 

  • Pasquini G, Faggioli F, Pilotti M, Lumia V, Barba M, Hadidi A (1998) Characterization of Apple chlorotic leaf spot virus isolates from Italy. Acta Hortic 472:195–202

    Article  CAS  Google Scholar 

  • Paunovic S, Ruzic D, Vujovic T, Milenkovic S, Ievremovic D (2007) In vitro production of Plum pox virus-free plums by chemotherapy with ribavirin. Biotechnol Biotechnol Equip 21:417–421

    Article  CAS  Google Scholar 

  • Quecini V, Lopes ML, Pacheco FTH, Ongarelli MDG (2006) Ribavirin, a guanosin analogue mammalian antiviral agent, impairs Tomato spotted wilt virus multiplication in tobacco cell cultures. Arch Phytopathol Plant Protect 41:1–13

    Article  CAS  Google Scholar 

  • Schwarz K, Jelkmann W (1998) Detection and characterization of European Apple stem pitting virus isolates of apple and pear by PCR and partial sequence analysis. Acta Hortic 472:75–85

    Article  CAS  Google Scholar 

  • Smith RA (1980) Mechanisms of action of ribavirin. In: Smith RA, Kirkpatrick W (eds) Ribavirin a Broad Spectrum Antiviral Agent. Academic Press, New York, pp 99–118

    Google Scholar 

  • Stouffer RF (1989) Apple stem pitting. In: Fridlund PR (ed) Virus and virus-like diseases of pome fruits and simulating non-infectious disorders. Washington State University, Pullman, pp 138–144

    Google Scholar 

  • Tan RR, Wang LP, Hong N, Wang GP (2010) Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell Tissue Organ Cult 101:229–235

    Article  Google Scholar 

  • Tiziano C, Folwell RJ, Wandschneider P, Eastwell KC, Howell WE (2003) Economic implications of a virus prevention program in deciduous tree fruits in the US. Crop Prot 22:1149–1156

    Article  Google Scholar 

  • Valero M, Ibáñez A, Morte A (2003) Effects of high vineyard temperatures on the Grapevine leafroll associated virus elimination from Vitis vinifera L. cv. Napoleon tissue cultures. Sci Hortic 97:289–296

    Article  Google Scholar 

  • Verma N, Ram R, Zaidi AA (2005) In vitro production of Prunus necrotic ringspot virus-free begonias through chemo-and thermotherapy. Sci Hortic 103:239–247

    Article  CAS  Google Scholar 

  • Wang LP, Wang GP, Hong N, Tang RR, Deng XY, Zhang H (2006) Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips. Hortscience 41:729–732

    Article  Google Scholar 

  • Wang QC, Cuellar WJ, Rajamäki M, Hirata Y, Valkonen JPT (2008) Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol Plant Pathol 8:1–14

    CAS  Google Scholar 

  • Welland CM, Cantos M, Troncoso A, Perez-Camacho F (2004) Regeneration of virus-free plants by in vitro chemotherapy of GFLV (Grapevine fanleaf virus) infected explants of Vitis vinifera L. cv. ‘Zalema’. Acta Hortic (ISHS) 652:463–466

    Article  Google Scholar 

  • Yanase H (1983) Back transmission of Apple stem grooving virus to apple seedlings and induction of symptoms of apple topworking disease in Mitsuba Kaido (Malus sieboldii) and Kobano Zumi (Malus sieboldii var. arborescens) rootstocks. Acta Hortic 130:117–122

    Article  Google Scholar 

Download references

Acknowledgements

This work is supported by the China Agriculture Research System CARS-29-10.

Author information

Authors and Affiliations

  1. National Key Laboratory of Agromicrobiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    G. J. Hu, N. Hong & G. P. Wang

  2. National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning, 125100, People’s Republic of China

    G. J. Hu

Authors
  1. G. J. Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. P. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. P. Wang.

Electronic supplementary material

ESM 1

(DOCX 16 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hu, G.J., Hong, N. & Wang, G.P. Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy. Australasian Plant Pathol. 48, 115–118 (2019). https://doi.org/10.1007/s13313-018-0606-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13313-018-0606-4

Keywords

Search

Navigation