Skip to main content
SpringerLink
Log in

Zinc supplementation induces resistance against root-rot in guar [Cyamopsis tetragonoloba (L.) Taub] seedlings

  • Original Paper
  • Published:
Australasian Plant Pathology Aims and scope Submit manuscript

Abstract

Soil-borne diseases are a major threat to guar cultivation all over the world. Many soil-borne fungi, including Rhizoctonia, infect plants causing damping-off and wilt diseases. Micronutrients play an important role in controlling the disease. This investigation was aimed to determine the effect of different concentrations of zinc (Zn2+) on biochemical parameters of guar, in relation to resistance against root rot. The guar seedlings were grown under 10 and 20 mg Zn2+ kg−1 soil, in earthen pots, containing inoculated soil. The inoculation of soil was done by the pretreatment of soil with Rhizoctonia inoculums. In the experiment, a control with uninoculated soil was also maintained. Lesion size was decreased by 35.1 and 46.8 % over control at Zn2+ 10 and 20 mg kg−1 soil treatments, respectively. Chlorophyll content, non-structural carbohydrate content and IVDMD (In Vitro Dry Matter Digestibility) per cent decreased in inoculated seedlings as compared to uninoculated seedlings. Phenol content and structural carbohydrates (Acid Detergent Fiber, cellulose, lignin and silica) increased in inoculated seedlings and this increase was further enhanced by Zn2+ 20 mg kg−1 soil treatment, to counteract the fungal invasion. Moreover, a significant increase in IVDMD was also observed at 20 mg Zn2+ kg−1 soil treatment. These results suggested that Zn2+ 20 mg kg−1 soil treatment might be used as soil-nutritive agent to provide resistance in plants against fungal diseases. The application of 20 mg Zn2+ kg−1 soil treatment could also prove useful in minimizing the use of fungicides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Alberto RT (2014) Pathogen response and biochemical changes in Allium cepa L. (bulb onions) infected with anthracnose-twister disease. Plant Pathol Quar 4:23–31

    Google Scholar 

  • Askar AAA, Rashad YM (2010) Arbuscular mycorrhizal fungi: a biocontrol agent against common bean Fusarium root rot disease. Plant Pathol J 9:31–38

    Article  Google Scholar 

  • Bajoria S, Varshney AK, Pareek RP, Mohan MK, Ghosh P (2008) Screening and characterization of antifungal clusterbean (Cyamopsis tetragonoloba) rhizobacteria. Biocontrol Sci Tech 18:139–156

    Article  Google Scholar 

  • Balbaa SI, Zaki AY, Glsharmy AM (1974) Total flavanol and rutin content of different organs of Soohora japonica L. J Assoc Anal Chem 57:752–755

    CAS  Google Scholar 

  • Barnes RF, Muller LD, Bauman LF, Colenbrander VF (1971) In vitro dry matter disappearance of brown midrib mutants of maize. J Anim Sci 33:881

    Article  Google Scholar 

  • Buttner G, Pfalher B, Marlander B (2004) Green house and field techniques for testing sugar beet for resistance to Rhizoctonia root and crown rot. Plant Breed 123:158–166

    Article  Google Scholar 

  • Cakmak I (2006) Effect of micronutrients on plant disease resistance. http://www.ipni.net/ppiweb/pbrazil.nsf/1c678d0ba742019483256e19004af5b8/51810fa28272d41003256fdd004d3ffd/$FILE/Anais%20Ismail%20Cakmak.pdf

  • Dhrub-Singh K, Nema KG, Singh D (1987) Effect of soil amendment on Rhizoctonia bataticola causing dry root rot of chickpea. Int Chick News 17:23–25

  • Dubois M, Gilles KA, Hamilton JJ, Rubress PA, Smith F (1956) Colorimetric method for determination of sugar and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Farkas GL, Kiraly Z (1962) Role of phenolic compounds in the physiology of plant diseases and disease resistance. J Phytopathol 44:105–150

    Article  CAS  Google Scholar 

  • Goering HK, Van Soest PJ, Smith F (1956) Forage fiber analysis. Agric. Hand Book No. 379, Washington, D.C., USDA

  • Hashem MSH, Farraq E (2009) Induction of resistance in Phaseolus vulgaris L.cv. Lima against some soil borne fungal pathogens by pre-inoculation with Tobacco Necrosis Virus (TNV) reacting hypersensitivity. Arch Phytopathol Plant Protect 42:676–687

    Article  CAS  Google Scholar 

  • Hassan MAE, Abo- Elyousr KAM (2013) Impact of compost application on Fusarium wilt disease incidence and microelements contents of basil plants. Arch Phytopathol Plant Protect 46:1904–1918

    Article  Google Scholar 

  • Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334

    Article  CAS  Google Scholar 

  • Huber DM, Haneklaus S (2007) Managing nutrition to control plant disease. Landbauforsch Volkenrode 57:313–322

    CAS  Google Scholar 

  • Johnson G, Schaal LA (1952) Relation of chlorogenic acid to scab resistance in potatoes. Science 115:627–629

    Article  CAS  PubMed  Google Scholar 

  • Kalim S (2003) Micronutrients induced effect on biochemical constituents in cowpea susceptible to Rhizoctonia solani and Rhizoctonia bataticola. M.Sc. Thesis, Haryana Agricultural University, Hisar

  • Kalra YP (1995) Determination of pH of soils by different methods: collaborative study. J AOAC Int 78:310–321

    CAS  Google Scholar 

  • Kataria HR, Yadav JPS, Grover RK (1981) Interaction of chemical fertilizers with seed dressing fungicides in controlling Rhizoctonia solani. Z Pflanzenkrankh Pflanzenschutz 88:641–650

    CAS  Google Scholar 

  • Khan AJ, Deadman ML, Srikandakumar A, Al-Maqbali YM, Rizvi SG, Al-Sabahi J (2001) Biochemical changes in sorghum leaves infected with leaf spot pathogen Drechslera sorghicola. J Plant Pathol 17:342–346

    Google Scholar 

  • Khoshgoftarmanesh AH, Kabiri S, Shariaimadari H, Sharifnabi B, Schulin R (2010) Zinc nutrition effect on the tolerance of wheat genotypes to Fusarium root rot disease in a solution culture experiment. Soil Sci Plant Nutr 56:234–243

    Article  CAS  Google Scholar 

  • Klikocka H (2009) Influence of NPK fertilization enriched with S, Mg and micronutrients contained in liquid fertilizer insol 7 on potato tubers yield (Solanum tuberosum L.) and infestation of tubes with Streptomyces scabies and Rhizoctonia solani. J Element 14:271–288

    Google Scholar 

  • Kosesakal T, Unal M (2009) Role of zinc deficiency in photosynthetic pigments and peroxidase activity of tomato seedlings. IUFS J Biol 68:113–120

    Google Scholar 

  • Kumar R, Singh R (1994) Tannins: their adverse role in ruminant nutrition. J Agric Food Chem 32:447–453

    Article  Google Scholar 

  • Kumar A, Mali PC, Manga VK (2010) Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola. Int J Plant Physiol Biochem 2:6–10

    CAS  Google Scholar 

  • Lattanzio V, Veronica M, Lattanzio T, Cardinali A (2006) Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Imperto F (ed) Phytochemistry: advances in research. Research Signpost, Kerala, pp. 23–67

    Google Scholar 

  • Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428

    Article  CAS  Google Scholar 

  • Luthra YP, Joshi UN, Singh SP (2009) Biomolecules involved in resistance against Chilo partellus (Swinehoe) in Sorghum bicolor L. Acta Phytopathol Entomol Hung 44:201–209

    Article  CAS  Google Scholar 

  • Madibela OR, Boitumelo WS, Letso M (2000) Chemical composition and in vitro dry matter digestibility of four parasitic plants (Tapinanthus lugardii, Erianthenum ngamicum, Viscum rotundifolium and Viscum verrucosum) in Botswana. Anim Feed Sci Technol 84:97–106

    Article  CAS  Google Scholar 

  • Neish AC (1964) Major pathways of biosynthesis of phenols. In: Harbone JB (ed) Biochemistry of phenolic compounds. Academic Press, New York, pp. 295–359

    Google Scholar 

  • Panse VG, Sukhatme PV (1954) Statistical methods for agricultural workers. Indian Council of Agricultural Research, New Delhi

    Google Scholar 

  • Patil SS, Dimond AE (1967) Inhibition of Verticillium polygalactouronase by oxidation products of polyphenol. Phytopathology 57:492–496

    CAS  PubMed  Google Scholar 

  • Peru RW, Main CB (1970) Chlorosis of tobacco induced by alternariol produced by A. tenuis. Phytopathology 60:1570–1573

    Article  Google Scholar 

  • Reddy MN, Rao AS (1978) Physiology of host parasite relations in damping-off of groundnut caused by Rhizoctonia solani. J Phytopathol 93:193–207

    Article  CAS  Google Scholar 

  • Reuveni M, Agapov V, Reuveni R (1997) A foliar spray of micronutrient solutions induces local and systemic protection against powdery mildew (Sphaerotheca fuliginea) in cucumber plants. Eur J Plant Pathol 103:581–588

    Article  CAS  Google Scholar 

  • Sequeira L (1983) Mechanisms of induced resistance in plants. Annu Rev Microbiol 37:51–79

    Article  CAS  PubMed  Google Scholar 

  • Simoglou K, Dordas C (2006) Effect of foliar applied boron, manganese and zinc on tan spot in winter durum wheat. Crop Prot 25:657–663

    Article  CAS  Google Scholar 

  • Somogyi N (1945) A new reagent for determination of sugars. J Biol Chem 160:61–69

    CAS  Google Scholar 

  • Spann TM, Schumann AW (2010) Mineral nutrition contributes to plant disease and pest resistance. UF/IFAS extension. pp: 1–5.

  • Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica: the quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68

    Article  CAS  Google Scholar 

  • Tilley JMA, Terry RA (1963) A two stage technique for the in vitro digestion of forage crop. J Brt Grassland Soc 18:104–111

    Article  CAS  Google Scholar 

  • Trouvelot S, Heloir MC, Poinssot B, Gauthier A, Paris F, Guillier C, Combier M, Trda L, Daire X, Adrian M (2014) Carbohydrates in plant immunity and plant protection: roles and potential application as foliar sprays. Front Plant Sci. doi:10.3389/fpls.2014.00592

    PubMed  PubMed Central  Google Scholar 

  • Vidhyasekaran P (1988) Physiology of disease resistance in plants, vol II. CRC Press, Florida, p. 128

    Google Scholar 

  • Walkley AJ, Black CA (1934) Estimation of soil organic carbon by the chromic acid titration method. Soil Sci 37:29–38

    Article  CAS  Google Scholar 

  • War AR, Paulraj MG, Ignacimuthu S, Sharma HC (2013) Defensive responses in groundnut against chewing and sap-sucking insects. J Plant Growth Regul 32:259–272

    Article  CAS  Google Scholar 

  • Yadav OP, Yadav TP, Kumar P, Gupta SK (1994) Inheritance of reducing sugars in relation to white rust resistance in Indian mustard. Ind Phytopathol 47:56–59

    CAS  Google Scholar 

  • Yang QY, Jia K, Geng WY, Guo RJ, Li SD (2014) Management of cucumber wilt disease by Bacillus subtilis B006 through suppression of Fusarium oxysporum f. sp. cucumerinum in rhizosphere. Plant Pathol J 13:160–166

    Article  Google Scholar 

Download references

Acknowledgments

The authors are highly indebted to Dr. S.K. Pahuja from Forage Section, Dr. S.K. Gandhi and Dr. Naresh Mehta from Department of Plant Pathology, CCS Haryana Agricultural University, Hisar for their co-operation and for providing all the necessary facilities during investigation.

Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana, 125004, India

    Neha Wadhwa & Udai Narayan Joshi

Authors
  1. Neha Wadhwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Udai Narayan Joshi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neha Wadhwa.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wadhwa, N., Joshi, U.N. Zinc supplementation induces resistance against root-rot in guar [Cyamopsis tetragonoloba (L.) Taub] seedlings. Australasian Plant Pathol. 45, 465–471 (2016). https://doi.org/10.1007/s13313-016-0439-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13313-016-0439-y

Keywords

Search

Navigation