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Identification of carboxyl terminal peptide of Fibrinogen as a potential serum biomarker for gastric cancer

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Tumor Biology

Abstract

Gastric cancer (GC) is a very common disease worldwide where new serum biomarkers are urgently needed to improve their early diagnosis. In this study, we aim to search for the potential serum protein/peptide biomarkers of GC by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). We first obtained the serum protein/peptide profiles from a training dataset including 30 patients with GC, 16 cases with chronic benign gastric disease (CGD), and 30 normal controls (CON) where 15 protein peaks were identified to exhibit the obvious deviation (P < 0.001, Wilcoxon rank sum test) among GC, CGD, and CON analyzed by Biomarker Wizard 3.1 software with three protein peaks with mass-to-charge (m/z) ratio 5910, 5342, and 6439 further confirmed in the validation dataset. Among the three protein peaks, peak 5910 displayed the most significantly different which could distinguish GC patients from CGD and CON with a sensitivity of 86.3 %, a specificity of 91.3 %, and the area under the receiver operating characteristic curve (AUC) of 0.89 by using the optimal cutoff value of 17.3. We further identified peak 5910 as the carboxyl terminal fraction of Fibrinogen α by LC-MS and validated its identity by antiserum-mediated SELDI-based immunodepletion assays. In sum, SELDI-TOF-MS method could effectively generate serum peptidome in cancer patients and provide a new approach to identify potentially diagnostic and prognostic biomarkers for cancer. The carboxyl terminal fraction of Fibrinogen α may be a potential serological biomarker for GC diagnosis.

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Acknowledgments

This work was supported by the grant from the National Natural Science Foundation of China (No. 81101644).

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    Authors and Affiliations

    1. Nanlou Digestive Endoscopy Center, Chinese PLA general hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China

      Cheng Wu & Zhiqiang Wang

    2. Department of Gastroenterology and Hepatology, The 306th Hospital of PLA, Beijing, China

      Zhiwen Luo

    3. Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai, 200433, China

      Cheng Wu, Zhiwen Luo, Dan Tang, Dingkang Yao & Liang Zhu

    4. Third Department of Geratology, The 401 hospital, PLA, Qingdao, China

      Lijie Liu

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    1. Cheng Wu
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    Correspondence to Liang Zhu or Zhiqiang Wang.

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    Cheng Wu and Zhiwen Luo contributed equally to this work.

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    Wu, C., Luo, Z., Tang, D. et al. Identification of carboxyl terminal peptide of Fibrinogen as a potential serum biomarker for gastric cancer. Tumor Biol. 37, 6963–6970 (2016). https://doi.org/10.1007/s13277-015-4394-y

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    • DOI: https://doi.org/10.1007/s13277-015-4394-y

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