Abstract
Many studies have been performed to assess potential utility of natural products as immunomodulants to enhance antitumor activity in situ. In this study, an essential oil (EO) from the aerial parts of Pituranthos tortuosus was prepared using hydrodistillation, its composition was characterized, and its immunomodulatory potential was assessed. The results indicated that the EO contained sabinene, α-pinene, limonene, and terpinen-4-ol as major constituents. EO was also found to be able to significantly promote lipopolysaccharide (LPS)-stimulated splenocyte proliferation, suggestive of a potential for activation of B cells and enhanced humoral immune responses in hosts given this product. Effects of EO on cell proliferation and apoptosis were also investigated in B16F10 melanoma cells. EO-induced tumor cell growth inhibition was associated with characteristic apoptotic changes in the cells, including nuclear condensation. In conclusion, these data suggested to us that an EO of P. tortuosus could evolve to be a potential medicinal resource for use in the treatment of cancers.
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Abdelwahed A, Hayder N, Kilani S, Mahmoud A, Chibani J, Hammami M, et al. Chemical composition and antimicrobial activity of essential oils from Tunisian Pituranthos tortuosus (Coss.) Maire. Flavour Fragrance J. 2006;21(1):129–33.
Krifa M, Gharad T, Haouala R. Biological activities of essential oil, aqueous and organic extracts of Pituranthos tortuosus (Coss.) Maire. Sci Hortic. 2011;128(1):61–7.
Abdallah HM, Ezzat SM. Effect of the method of preparation on the composition and cytotoxic activity of the essential oil of Pituranthos tortuosus. Z Naturforsch C. 2011;66(3–4):143–8.
Abdelwahed A, Skandrani I, Kilani S, Neffati A, Sghaier MB, Bouhlel I, et al. Mutagenic, antimutagenic, cytotoxic, and apoptotic activities of extracts from Pituranthos tortuosus. Drug Chem Toxicol. 2008;31(1):37–60.
Jelassi A, Cheraief I, Hamza MA, Jannet HB. Chemical composition and characteristic profiles of seed oils from three Tunisian Acacia species. J Food Compos Anal. 2014;33(1):49–54.
Krifa M, Bouhlel I, Ghedira-Chekir L, Ghedira K. Immunomodulatory and cellular anti-oxidant activities of an aqueous extract of Limoniastrum guyonianum gall. J Ethnopharmacol. 2013;146(1):243–9.
Krifa M, Mustapha N, Ghedira Z, Ghedira K, Pizzi A, Chekir-Ghedira L. Limoniastrum guyonianum methanol extract induces immunomodulatory and anti-inflammatory effects by activating cellular anti-oxidant activity. Drug Chem Toxicol. 2015;38 (1): 84-91.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63.
Manosroi A, Saraphanchotiwitthaya A, Manosroi J. Immunomodulatory activities of Clausena excavata Burm. f. wood extracts. J Ethnopharmacol. 2003;89(1):155–60.
Youn MJ, Kim JK, Park SY, Kim Y, Park C, Kim ES, et al. Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells. J Ethnopharmacol. 2009;121(2):221–8.
D'Agostini C, Pica F, Febbraro G, Grelli S, Chiavaroli C, Garaci E. Antitumour effect of OM-174 and cyclophosphamide on murine B16 melanoma in different experimental conditions. Int Immunopharmacol. 2005;5(7–8):1205–12.
Marciani DJ, Press JB, Reynolds RC, Pathak AK, Pathak V, Gundy LE, et al. Development of semisynthetic triterpenoid saponin derivatives with immune stimulating activity. Vaccine. 2000;18(27):3141–51.
Zhang J, Sun R, Wei H, Tian Z. Antitumor effects of recombinant human prolactin in human adenocarcinoma-bearing SCID mice with human NK cell xenograft. Int Immunopharmacol. 2005;5(2):417–25.
Kim KD, Choi SC, Kim A, Choe YK, Choe IS, Lim JS. Dendritic cell-tumor coculturing vaccine can induce antitumor immunity through both NK and CTL interaction. Int Immunopharmacol. 2001;1(12):2117–29.
Han SB, Kim YH, Lee CW, Park SM, Lee HY, Ahn KS, et al. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology. 1998;40(1):39–48.
Manosroi A, Saraphanchotiwitthaya A, Manosroi J. In vitro immunomodulatory effect of Pouteria cambodiana (Pierre ex Dubard) Baehni extract. J Ethnopharmacol. 2005;101(1–3):90–4.
Krifa M, Skandrani I, Pizzi A, Nasr N, Ghedira Z, Mustapha N, et al. An aqueous extract of Limoniastrum guyonianum gall induces anti-tumor effects in melanoma-injected mice via modulation of the immune response. Food Chem Toxicol. 2014;69C:76–85.
Greay SJ, Ireland DJ, Kissick HT, Levy A, Beilharz MW, Riley TV, et al. Induction of necrosis and cell cycle arrest in murine cancer cell lines by Melaleuca alternifolia (tea tree) oil and terpinen-4-ol. Cancer Chemother Pharmacol. 2009;65(5):877–88.
Lu XG, Zhan LB, Feng BA, Qu MY, Yu LH, Xie JH. Inhibition of growth and metastasis of human gastric cancer implanted in nude mice by d-limonene. World J Gastroenterol. 2004;10(14):2140–4.
Acknowledgments
This work was supported by the Erasmus Mundus action 2 Lot 6 EGOVTN project no. 2013-2434/001-001.
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The authors declare no conflicts of interest. The authors alone are responsible for the content of this manuscript.
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Krifa, M., El Mekdad, H., Bentouati, N. et al. Immunomodulatory and anticancer effects of Pituranthos tortuosus essential oil. Tumor Biol. 36, 5165–5170 (2015). https://doi.org/10.1007/s13277-015-3170-3
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DOI: https://doi.org/10.1007/s13277-015-3170-3