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Immunohistochemically detected expression of Skp2, p27kip1, and p-p27 (Thr187) in patients with cholangiocarcinoma

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Tumor Biology

Abstract

The paper was aimed to detect the expression of Skp2, p27kip1 (p27), and p-p27 (Thr187) in patients with cholangiocarcinoma (CCA). Western blot and immunohistochemistry were used to analyze the expression and subcellular localization of p27, Skp2, and p-p27 (Thr187) in tissue specimens of 53 patients with CCA and 10 with chronic proliferative cholangitis (CPC). Besides, the relationships of p27, Skp2, and p-p27 (Thr187) with clinicopathologic findings were examined, followed by analysis of the relationships of p27 expression with p-p27 (Thr187) and Skp2 in CCA tissue. The expression of p27 was lower in CCA than CPC tissue, and the expression of Skp2 and p-p27 (Thr187) was higher in CCA than CPC tissue (P < 0.05). The positive p27 and Skp2 proteins were mainly expressed in nucleus and cytoplasm of CCA, and p-p27 (Thr187) was only observed in nucleus. The expression of p27, Skp2, and p-p27 (Thr187) was closely associated with tumor grade and TNM stage (P < 0.05). A significantly negative correlation between the expression of p27 and Skp2 (r = −0.480, P < 0.05) and between the expression of p27 and p-p27 (Thr187) (r = −0.387, P < 0.05) was observed. Skp2-dependent proteasomal degradation of p27 plays role in the malignant transformation of CCA. Besides, the expression of p27, Skp2, and p-p27 (Thr187) may serve as markers for the progression of CCA.

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Authors and Affiliations

  1. Integrated Dept, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, Hankou District, Wuhan, 430030, Hubei Province, China

    Jian Luo & Hongzhen Lan

  2. Gastrointestinal Surgery Dept, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, China

    Yi Zhou

  3. Bile Duct and Pancreatic Surgery Dept, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, China

    Bing Wang & Yongjun Chen

  4. Gynaecology and Obstetrics Dept, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, China

    Qiang Li

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  1. Jian Luo
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Correspondence to Jian Luo.

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Luo, J., Zhou, Y., Wang, B. et al. Immunohistochemically detected expression of Skp2, p27kip1, and p-p27 (Thr187) in patients with cholangiocarcinoma. Tumor Biol. 36, 5119–5125 (2015). https://doi.org/10.1007/s13277-015-3164-1

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  • DOI: https://doi.org/10.1007/s13277-015-3164-1

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