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SCC-S2 is overexpressed in colon cancers and regulates cell proliferation

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Tumor Biology

Abstract

SCC-S2 was recently reported to be overexpressed in various cancers and associated with the malignant behavior of cancer cells. However, the expression of SCC-S2 and its biological roles in colon cancers have not been reported. The aim of this study is to investigate the expression and clinical significance of SCC-S2 in colon cancers and explore its biological function in colon cancer cells. We analyzed the expression pattern of SCC-S2 in 92 colon cancer tissues by immunohistochemistry and found that SCC-S2 was overexpressed in 45 (48.9 %) of 92 colon cancer specimens. There was a significant association between SCC-S2 overexpression and TNM stage (p = 0.0387), lymph node metastasis (p = 0.0219), and proliferation index (p = 0.0279). siRNA knockdown of SCC-S2 expression in CACO2 and HCT116 cells decrease cell proliferation, colony formation, and soft agar colony formation ability. Furthermore, western blot analysis showed that SCC-S2 depletion decreased cyclin D1 and phospho-Rb levels. In conclusion, we demonstrated that SCC-S2 is overexpressed in colon cancers and contributes to malignant cell growth by cyclin D1 and phospho-Rb modulation, which makes SCC-S2 a candidate therapeutic target in colon cancer.

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Authors and Affiliations

  1. Department of General Surgery, First Affiliated Hospital of China Medical University, Shenyang, China

    Zhifeng Miao, Tingting Zhao, Zhenning Wang, Yingying Xu, Yongxi Song, Jianhua Wu & Huimian Xu

  2. Department of Surgical Oncology and General Surgery, First affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China

    Huimian Xu

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  1. Zhifeng Miao
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  2. Tingting Zhao
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  3. Zhenning Wang
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  4. Yingying Xu
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  5. Yongxi Song
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  6. Jianhua Wu
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  7. Huimian Xu
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Correspondence to Huimian Xu.

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Miao, Z., Zhao, T., Wang, Z. et al. SCC-S2 is overexpressed in colon cancers and regulates cell proliferation. Tumor Biol. 33, 2099–2106 (2012). https://doi.org/10.1007/s13277-012-0469-1

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  • DOI: https://doi.org/10.1007/s13277-012-0469-1

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