Abstract
The aim of this study is to assess the expression levels of SMYD2 in human tissue samples and cells of colon cancer, and further explore the potential mechanisms of SMYD2 in colon cancer progression. Quantitative PCR and Immunohistochemical (IHC) assays were performed to detect SMYD2 expression in 76 tissue samples of colon cancer tissues and the corresponding normal tissues. The potential correlations between SMYD2 expression levels and clinical pathological features were assessed. We further detected the effects of SMYD2 on the proliferation, invasion and apoptosis of colon cancer cells and on ERBB2/FUT4 signaling pathway through Brdu assay, transwell assay and flow cytometry assay, respectively. The potential effects of SMYD2 on tumor growth were explored using an animal model. We demonstrated the possible involvement of SMYD2 in the progression of colon cancer. We found the high expression of SMYD2 in human colon cancer tissues and cells, and found the correlations between SMYD2 expression and the clinicopathological features including vascular invasion (P = 0.007*), TNM stage (P = 0.016*) and lymph node metastasis (P = 0.011*), of patients with colon cancer. Our data further confirmed that SMYD2 affects cell proliferation, invasion, and apoptosis of colon cancer cells via the regulation of ERBB2/FUT4 signaling pathway. We also demonstrated SMYD2 contributed to tumor growth of colon cancer cells in vivo. We investigated the potential involvement of SMYD2 in the progression of colon, and therefore confirmed SMYD2 as a possible therapeutic target for colon cancer.
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YY conceived and designed the experiments, YZL analyzed and interpreted the results of the experiments, and YZL and YY performed the experiments.
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Tumor tissues were obtained from Chongqing University Cancer Hospital. All procedures performed in studies involving human participants were in accordance with the standards upheld by the Ethics Committee of Chongqing University Cancer Hospital and with those of the 1964 Helsinki Declaration and its later amendments for ethical research involving human subjects. All animal assay processes were approved by the Ethics Committee of Chongqing University for the use of animals and conducted in accordance with the National Institutes of Health Laboratory Animal Care and Use Guidelines.
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Lai, Y., Yang, Y. SMYD2 facilitates cancer cell malignancy and xenograft tumor development through ERBB2-mediated FUT4 expression in colon cancer. Mol Cell Biochem 477, 2149–2159 (2022). https://doi.org/10.1007/s11010-020-03738-2
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DOI: https://doi.org/10.1007/s11010-020-03738-2