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Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines

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Abstract

Background

Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported.

Methods

A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis.

Results

The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure.

Conclusion

Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.

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References

  • Baggerman G, Vierstraete E, De Loof A, Schoofs L (2005) Gel-based versus gel-free proteomics: a review. Comb Chem High Throughput Screen 8:669–677

    CAS  PubMed  Google Scholar 

  • Bai B et al (2019) Peroxiredoxin2 downregulation enhances hepatocellular carcinoma proliferation and migration, and is associated with unfavorable prognosis in patients. Oncol Rep 41:1539–1548

    CAS  PubMed  PubMed Central  Google Scholar 

  • Caballero JP et al (2019) Nanoencapsulated deltamethrin as synergistic agent potentiates insecticide effect of indoxacarb through an unusual neuronal calcium-dependent mechanism. Pestic Biochem Physiol 157:1–12

    Google Scholar 

  • Cabello G et al (2001) A rat mammary tumor model induced by the organophosphorous pesticides parathion and malathion, possibly through acetylcholinesterase inhibition. Environ Health Perspect 109:471–479

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cao W et al (2018) Determination of deltamethrin and its toxicity biomarkers in rabbit urine by high performance liquid chromatography-tandem mass spectrometry. Se Pu 36:523–530

    CAS  PubMed  Google Scholar 

  • Chauvin S, Sobel A (2015) Neuronal stathmins: a family of phosphoproteins cooperating for neuronal development, plasticity and regeneration. Prog Neurobiol 126:1–18

    CAS  PubMed  Google Scholar 

  • Chen M et al (2019) Molecular evidence of sequential evolution of DDT-and pyrethroid-resistant sodium channel in aedes aegypti. PLoS Negl Trop Dis 13:e0007432

    PubMed  PubMed Central  Google Scholar 

  • Chrustek A et al (2018) Current research on the safety of pyrethroids used as insecticides. Medicina (Kaunas) 54:61

    Google Scholar 

  • Cook JA, Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal Biochem 179:1–7

    CAS  PubMed  Google Scholar 

  • Danh HC, Benedetti MS, Dostert P (1983) Differential changes in superoxide dismutase activity in brain and liver of old rats and mice. J Neurochem 40:1003–1007

    CAS  PubMed  Google Scholar 

  • Ding R et al (2017) The implication of p66shc in oxidative stress induced by deltamethrin. Chem Biol Interact 278:162–169

    CAS  PubMed  Google Scholar 

  • Dinis-Oliveira RJ et al (2006) Acute paraquat poisoning: report of a survival case following intake of a potential lethal dose. Pediatr Emerg Care 22:537–540

    PubMed  Google Scholar 

  • Doi H et al (2006) Motor neuron disorder simulating ALS induced by chronic inhalation of pyrethroid insecticides. Neurology 67:1894–1895

    CAS  PubMed  Google Scholar 

  • El Golli-Bennour E et al (2019) Protective effects of kegir against deltamethrin-induced hepatotoxicity in rats. Environ Sci Pollut Res Int 26:18856–18865

    PubMed  Google Scholar 

  • Ellman GL, Courtney KD, Andres V Jr, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95

    CAS  PubMed  Google Scholar 

  • Ensibi C et al (2013) Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environ Toxicol Pharmacol 36:384–391

    CAS  PubMed  Google Scholar 

  • Flohé L, Günzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114–120

    PubMed  Google Scholar 

  • Fulton MH, Key PB (2001) Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environ Toxicol Chem 20:37–45

    CAS  PubMed  Google Scholar 

  • Gautrey H et al (2015) SRSF3 and hnRNP H1 regulate a splicing hotspot of HER2 in breast cancer cells. RNA Biol 12:1139–1151

    PubMed  PubMed Central  Google Scholar 

  • Glorieux C, Calderon PB (2017) Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem 398:1095–1108

    CAS  PubMed  Google Scholar 

  • Hadwan MH (2018) Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem 19:1–8

    Google Scholar 

  • Hileman EA, Achanta G, Huang P (2001) Superoxide dismutase: an emerging target for cancer therapeutics. Expert Opin Ther Targets 5:697–710

    CAS  PubMed  Google Scholar 

  • Ingkaninan K, Temkitthawon P, Chuenchom K, Yuyaem T, Thongnoi W (2003) Screening for acetylcholinesterase inhibitory activity in plants used in thai traditional rejuvenating and neurotonic remedies. J Ethnopharmacol 89:261–264

    PubMed  Google Scholar 

  • Jia ZZ, Zhang JW, Zhou D, Xu DQ, Feng XZ (2019) Deltamethrin exposure induces oxidative stress and affects meiotic maturation in mouse oocyte. Chemosphere 223:704–713

    CAS  PubMed  Google Scholar 

  • Kumar A, Sharma R, Rana D, Sharma N (2019a) Protective effect of alpha-tocopherol in deltamethrin induced immunotoxicity. Endocr Metab Immune Disord Drug Targets 19:171–184

    CAS  PubMed  Google Scholar 

  • Kumar A, Gupta M, Sharma R, Sharma N (2019b) Deltamethrin induced immunotoxicity and its protection by quercetin: an experimental study. Endocr Metab Immune Disord Drug Targets 19:1

    Google Scholar 

  • Lu W et al (2014) Peroxiredoxin 2 is upregulated in colorectal cancer and contributes to colorectal cancer cells’ survival by protecting cells from oxidative stress. Mol Cell Biochem 387:261–270

    CAS  PubMed  Google Scholar 

  • Lu Q et al (2018) Deltamethrin toxicity: a review of oxidative stress and metabolism. Environ Res 170:260–281

    PubMed  Google Scholar 

  • Mannervik B (1999) Measurement of glutathione reductase activity. Curr Protoc Toxicol 7:1–4

    Google Scholar 

  • Min CW et al (2017) Gel-based and gel-free proteome data associated with controlled deterioration treatment of glycine max seeds. Data Br 15:449–453

    Google Scholar 

  • Nakamura M et al (2006) Phosphoproteomic profiling of human SH-SY5Y neuroblastoma cells during response to 6-hydroxydopamine-induced oxidative stress. Biochim Biophys Acta 1763:977–989

    CAS  PubMed  Google Scholar 

  • Oda SS, El-Maddawy ZK (2012) Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Exp Toxicol Pathol 64:813–819

    CAS  PubMed  Google Scholar 

  • Pitzer EM et al (2019) Deltamethrin exposure daily from postnatal day 3–20 in sprague-dawley rats causes long-term cognitive and behavioral deficits. Toxicol Sci 169:511–523

    CAS  PubMed  Google Scholar 

  • Ríos JC et al (2003) Tribromophenol induces the differentiation of SH-SY5Y human neuroblastoma cells in vitro. Toxicol In Vitro 17:635–641

    PubMed  Google Scholar 

  • Rubin CI, Atweh GF (2004) The role of stathmin in the regulation of the cell cycle. J Cell Biochem 93:242–250

    CAS  PubMed  Google Scholar 

  • Ryu AR, Kim YW, Lee MY (2019) Chlorin e6 and halogen light as a sebostatic photomedicine modulates linoleic acid-induced lipogenesis. Mol Cell Toxicol 15:49–56

    CAS  Google Scholar 

  • Shim DH, Lim JW, Kim H (2015) Differentially expressed proteins in nitric oxide-stimulated NIH/3T3 fibroblasts: implications for inhibiting cancer development. Yonsei Med J 56:563–571

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shishkin SS, Kovalev LI, Pashintseva NV, Kovaleva MA, Lisitskaya K (2019) Heterogeneous nuclear ribonucleoproteins involved in the functioning of telomeres in malignant cells. Int J Mol Sci 20:745

    CAS  PubMed Central  Google Scholar 

  • Shrivastava R, Köster D, Kalme S, Mayor S, Neerathilingam M (2015) Tailor-made ezrin actin binding domain to probe its interaction with actin in-vitro. PLoS One 10:1–12

    Google Scholar 

  • Skandrani D et al (2003) Effect of selected insecticides on growth rate and stress protein expression in cultured human A549 and SH-SY5Y cells. Toxicol In Vitro 20:1378–1386

    Google Scholar 

  • Souza MF et al (2018) Deltamethrin intranasal administration induces memory, emotional and tyrosine hydroxylase immunoreactivity alterations in rats. Brain Res Bull 142:297–303

    CAS  PubMed  Google Scholar 

  • Suenaga S et al (2013) Human pancreatic cancer cells with acquired gemcitabine resistance exhibit significant up-regulation of peroxiredoxin-2 compared to sensitive parental cells. Anticancer Res 33:4821–4826

    CAS  PubMed  Google Scholar 

  • Traverso N et al (2013) Role of glutathione in cancer progression and chemoresistance. Oxidative Med Cell Longev 2013:1–10

    Google Scholar 

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Acknowledgements

This study was supported by Soonchunhyang University.

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Authors and Affiliations

  1. Department of Medical Science, Soonchunhyang University, Asan, Chungnam, 31538, Korea

    Min-Joo Kang & Mi-Young Lee

  2. Department of Medical Biotechnology, Soonchunhyang University, Asan, Chungnam, 31538, Korea

    Mi-Young Lee

Authors
  1. Min-Joo Kang
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  2. Mi-Young Lee
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Contributions

MJK performed the experiments and wrote the initial draft of manuscript. MYL designed the research and wrote the final version of manuscript.

Corresponding author

Correspondence to Mi-Young Lee.

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Min-Joo Kang and Mi-Young Lee declare that they have no conflict of interest.

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The article does not contain any studies with humans or animals and this study was performed following institutional and national guidelines.

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Kang, MJ., Lee, MY. Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines. Mol. Cell. Toxicol. 16, 93–101 (2020). https://doi.org/10.1007/s13273-019-00064-7

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  • DOI: https://doi.org/10.1007/s13273-019-00064-7

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