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Effect of Prunus yedoensis Matsumura extract on the gene expression in HaCaT cells

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Abstract

Backgrounds

The bark of Prunus yedoensis (PY) is used in herbal remedies for the treatment of inflammatory skin disorders. Previous studies have reported the antioxidative, anti-inflammatory, anticancer, and vasorelaxant effects of PY; however, the safety and toxicity of PY extracts have not yet been reported. In the present study, we explored whether PY bark extract induced toxicity in HaCaT, a human keratinocyte cell line.

Methods

HaCaT cells were treated with PYE, the ethyl acetate (EtOAc) fraction of the crude extract (produced from 80% EtOH) of PY, for 24 h and cytotoxicity and ROS generation were determined. In addition, we evaluated the effect of a high concentration of PYE on the transcriptional profile to determine the action mechanism of the toxicity to HaCaT cells.

Results

PYE treatment significantly induced cytotoxicity in HaCaT cells in a dose-dependent manner. At 313 μg/mL, PYE generated intracellular ROS and up-regulated immediate early genes and oxidative stress-related genes, indicating the activation of stress signals by PYE. Moreover, PYE significantly changed the expression of genes that promote ECM degradation, inflammatory responses, and keratinocyte differentiation.

Conclusion

The present study demonstrated that PYE at 313 μg/mL, a concentration which is not cytotoxic but generates intracellular ROS, affected gene regulation in HaCaT human keratinocytes through multiple toxicity mechanisms and could therefore disturb skin homeostasis or lead to skin aging. We propose that for the safe use of PYE in herbal cosmetic or medicines, further evaluations of the toxicity and safety in the skin must be performed.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Daejin University, Gyeonggi, 11159, Republic of Korea

    Shin-Youn Joo

  2. Department of Cosmetic Science & Management, Graduate School, Incheon National University, Incheon, 22012, Republic of Korea

    Yuna Ha, Ga Young Seo & Youn-Jung Kim

  3. Department of Marine Sciences, Incheon National University, Incheon, 22012, Republic of Korea

    Do Yeon Seo & Youn-Jung Kim

  4. Department of Food Service Management and Nutrition, Kongju National University, Yesan, 32439, Republic of Korea

    Hae-Yeon Choi

Authors
  1. Shin-Youn Joo
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  2. Yuna Ha
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  3. Do Yeon Seo
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  4. Ga Young Seo
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  5. Youn-Jung Kim
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  6. Hae-Yeon Choi
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Corresponding authors

Correspondence to Youn-Jung Kim or Hae-Yeon Choi.

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Joo, SY., Ha, Y., Seo, D.Y. et al. Effect of Prunus yedoensis Matsumura extract on the gene expression in HaCaT cells. Mol. Cell. Toxicol. 14, 337–345 (2018). https://doi.org/10.1007/s13273-018-0037-x

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  • DOI: https://doi.org/10.1007/s13273-018-0037-x

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