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Identification of atrophy-related proteins produced in response to cast immobilization in rat gastrocnemius muscle

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Abstract

Prolonged disuse of skeletal muscle causes atrophy, which is a universal phenomenon induced by various factors such as cast immobilization and space flight. However, the identity of proteins produced in atrophying skeletal muscle cells is poorly understood, especially during a period of cast immobilization. In the present study, we used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry to investigate protein expression in rat gastrocnemius subjected to cast immobilization for 7 and 21 days. Gastrocnemius muscle mass was lost continuously throughout the 21 days of cast immobilization. Proteomic analysis of silver-stained gels of whole-protein extracts from rat gastrocnemius muscle strips detected 48 proteins. Of these proteins, the expression of 6 proteins changed during cast immobilization; these proteins are involved in metabolic, contraction, and chaperone activities. These results suggest that cast immobilization-induced skeletal muscle atrophy is related to changes in the defense and contractile apparatus proteins in rat gastrocnemius muscle.

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Authors and Affiliations

  1. Department of Physical Therapy, College of Public Health & Welfare, Yongin University, Yongin, 449-714, Korea

    Junghwan Kim

  2. Department of Physiology, Institute of Functional Genomics, School of Medicine, Konkuk University, Chungju, 380-701, Korea

    Bokyung Kim

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  1. Junghwan Kim
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Correspondence to Junghwan Kim.

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Kim, J., Kim, B. Identification of atrophy-related proteins produced in response to cast immobilization in rat gastrocnemius muscle. Mol. Cell. Toxicol. 6, 359–369 (2010). https://doi.org/10.1007/s13273-010-0048-8

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  • DOI: https://doi.org/10.1007/s13273-010-0048-8

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