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Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells

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Cell Stress and Chaperones Aims and scope

Abstract

Exposure to 17β-estradiol prior to induction of apoptosis protects skeletal muscle cells against damage. The mechanism involved in this protective action of the hormone is poorly understood. In the present study, using the murine muscle cell line C2C12, evidence was obtained that inhibition of H2O2-induced apoptosis by the estrogen requires the participation of heat shock protein 27 (HSP27). Reverse transcriptase polymerase chain reaction, Western blot, and immunocytochemistry assays showed that 17β-estradiol induces a time-dependent (5–60 min) increase in the expression of HSP27. In addition, in presence of quercetin, an inhibitor of HSPs, the antiapoptotic effect of the hormone was diminished. More specifically, blockage experiments with short interference RNA targeting HSP27 confirmed the role of this chaperone in the protective effect of the steroid. 17β-Estradiol abolished caspase-3 cleavage elicited by H2O2. Coimmunoprecipitation assays suggested physical interaction of HSP27 with caspase-3 in presence of estradiol. Furthermore, we observed that this chaperone interacts with estrogen receptors (ER) β in mitochondria. Then, this study suggests that HSP27 plays a new role in the antiapoptotic action triggered by 17β-estradiol by modulating caspase-3 activity and stabilizing ERβ in skeletal muscle cells.

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Acknowledgments

This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica and the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.

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Authors and Affiliations

  1. Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000, Bahía Blanca, Argentina

    Andrea Vasconsuelo, Lorena Milanesi & Ricardo Boland

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  1. Andrea Vasconsuelo
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  2. Lorena Milanesi
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  3. Ricardo Boland
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Correspondence to Andrea Vasconsuelo.

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Vasconsuelo, A., Milanesi, L. & Boland, R. Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells. Cell Stress and Chaperones 15, 183–192 (2010). https://doi.org/10.1007/s12192-009-0132-y

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  • DOI: https://doi.org/10.1007/s12192-009-0132-y

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