Skip to main content
SpringerLink
Log in

The effect of the PLIN1 gene on the metabolism and mitochondria of porcine skeletal muscle satellite cells

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

Background

Perilipin 1 (PLIN1) is a lipid droplet scaffolding protein that plays a regulatory role in fat decomposition and mitochondrial function.

Objective

In this study, the effects of PLIN1 gene knockout (PLIN1-KO) and PLIN1 gene overexpression (PLIN1-EX) on cell metabolism and mitochondrial function in porcine skeletal muscle satellite cells were assessed.

Methods

Porcine skeletal muscle satellite cells were used as the control group (NC). The expression of mitochondrial function-related proteins was detected by western blot. Apoptosis, cell cycle, mitochondrial function-related indices, mitochondrial structure, and morphology were measured by flow cytometry.

Results

Our results demonstrated that stable expression of the PLIN1 gene in skeletal muscle satellite cells is critical to maintaining cell metabolism and mitochondrial function. After knockout and overexpression of the PLIN1 gene, the anti-apoptotic ability of cells was enhanced, and the metabolic activity of the cells was accelerated, but at the cost of mitochondrial structural damage, reduction in the number of mitochondria, and decreased mitochondrial function.

Conclusion

This study explored the effect of the PLIN1 gene on the mitochondria and metabolism of porcine skeletal muscle satellite cells and provided a theoretical basis for the subsequent study of the effects of PLIN1 on muscle tissue development and meat quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Alsayyah C, Ozturk O, Cavellini L, Belgareh-Touze N, Cohen MM (2020) The regulation of mitochondrial homeostasis by the ubiquitin proteasome system. Biochim Biophys Acta Bioenerg 1861:148302

    Article  CAS  PubMed  Google Scholar 

  • Chandhok G, Lazarou M, Neumann B (2018) Structure, function, and regulation of mitofusin-2 in health and disease. Biol Rev Camb Philos Soc 93:933–949

    Article  PubMed  Google Scholar 

  • Floyd ZE, Stephens JM (2012) Controlling a master switch of adipocyte development and insulin sensitivity: covalent modifications of PPARgamma. Biochim Biophys Acta 1822:1090–1095

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Granneman JG, Moore HP, Krishnamoorthy R, Rathod M (2009) Perilipin controls lipolysis by regulating the interactions of AB-hydrolase containing 5 (Abhd5) and adipose triglyceride lipase (Atgl). J Biol Chem 284:34538–34544

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo L, Cui H, Zhao G, Liu R, Li Q, Zheng M, Guo Y, Wen J (2018) Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens. BMC Genom 19:838

    Article  CAS  Google Scholar 

  • Ju H, Zhang J, Bai L, Mu Y, Du Y, Yang W, Li Y, Sheng A, Li K (2015) The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production. Sci Rep 5:10152

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kimmel AR, Brasaemle DL, McAndrews-Hill M, Sztalryd C, Londos C (2010) Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins. J Lipid Res 51:468–471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kishikawa JI, Inoue Y, Fujikawa M, Nishimura K, Nakanishi A, Tanabe T, Imamura H, Yokoyama K (2018) General anesthetics cause mitochondrial dysfunction and reduction of intracellular ATP levels. PLoS ONE 13:e0190213

    Article  PubMed  PubMed Central  Google Scholar 

  • Konig SM, Rissler V, Terkelsen T, Lambrughi M, Papaleo E (2019) Alterations of the interactome of Bcl-2 proteins in breast cancer at the transcriptional, mutational and structural level. PLoS Comput Biol 15:e1007485

    Article  PubMed  PubMed Central  Google Scholar 

  • Li B, Weng Q, Dong C, Zhang Z, Li R, Liu J, Jiang A, Li Q, Jia C, Wu W et al (2018) A key gene, PLIN1, can affect porcine intramuscular fat content based on transcriptome analysis. Genes (basel) 9:194

    Article  CAS  PubMed  Google Scholar 

  • Mauro A (1961) Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 9:493–495

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mazzali G, Fantin F, Zoico E, Sepe A, Bambace C, Faccioli S, Pedrotti M, Corzato F, Rizzatti V, Faggian G et al (2015) Heart fat infiltration in subjects with and without coronary artery disease. J Clin Endocrinol Metab 100:3364–3371

    Article  CAS  PubMed  Google Scholar 

  • Meng LX, Zheng YX, He ML, Zhou XM, Sun SY, Ding ZJ, Meng Q, Li BC, Sun YW (2018) Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Mol Med Rep 18:4571–4576

    CAS  PubMed  Google Scholar 

  • Shefer G, Wleklinski-Lee M, Yablonka-Reuveni Z (2004) Skeletal muscle satellite cells can spontaneously enter an alternative mesenchymal pathway. J Cell Sci 117:5393–5404

    Article  CAS  PubMed  Google Scholar 

  • Siersbaek MS, Loft A, Aagaard MM, Nielsen R, Schmidt SF, Petrovic N, Nedergaard J, Mandrup S (2012) Genome-wide profiling of peroxisome proliferator-activated receptor gamma in primary epididymal, inguinal, and brown adipocytes reveals depot-selective binding correlated with gene expression. Mol Cell Biol 32:3452–3463

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sztalryd C, Brasaemle DL (2017) The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids 1862:1221–1232

    Article  CAS  PubMed  Google Scholar 

  • Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Sci 78:343–358

    Article  CAS  PubMed  Google Scholar 

  • Yang K, Yang Y, Qi C, Ju H (2020) Effects of porcine STC-1 on cell metabolism and mitochondrial function. Gen Comp Endocrinol 286:113298

    Article  CAS  PubMed  Google Scholar 

  • Zhao Z, Wang J, Ma M (2016) Down regulation of Perilipin 1 gene expression in 3T3-L1cell lipolysis. Chin J Bioeng 36:17–22

    Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31872323, 31272405), the Natural Science Foundation of Jiangsu (BK20201224), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Jiangsu Co-innovation Center for Prevention. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Author information

Author notes

  1. Yi Wang, Yuehan Peng and Yuefei Yang have contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Yangzhou University/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, 225009, Jiangsu, People’s Republic of China

    Yi Wang, Yuehan Peng, Yuefei Yang, Yuemin Hu, Hao Wang, Lei Xu & Huiming Ju

Authors
  1. Yi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuehan Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuefei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuemin Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Huiming Ju
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

HJ, YW, and YP planned the experiments. HW, YY, and YP designed and performed the molecular experiment. HW, YY, and LX designed and performed the cell experiments. HW, YY, and YH performed the data analysis. All authors edited the manuscript.

Corresponding author

Correspondence to Huiming Ju.

Ethics declarations

Conflict of interest

Author Yi Wang, author Yuehan Peng, author Yuefei Yang, author Yuemin Hu, author Hao Wang, author Lei Xu and author Huiming Ju declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, Y., Peng, Y., Yang, Y. et al. The effect of the PLIN1 gene on the metabolism and mitochondria of porcine skeletal muscle satellite cells. Genes Genom 44, 1555–1563 (2022). https://doi.org/10.1007/s13258-022-01252-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-022-01252-x

Keywords

Search

Navigation