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Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in non-heading Chinese cabbage

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Abstract

WS24-3 is a newly bred recessive genic male sterility line of the non-heading Chinese cabbage (Brassica rapa ssp. chinensis). Here, an F2 population was produced from the cross between WS24-3 and a male-fertile breeding line (WS135). The Illumina Brassica 60 K single nucleotide polymorphism (SNP) array was used for SNPs detecting between sterile and fertile bulks from the F2 population, and 62 SNPs were identified. BLAST analysis of the 62 SNPs revealed that the A2 chromosome of Brassica rapa genome contained 22 SNPs, whereas the other chromosomes did not contain more than 6 SNPs each. These data indicated that the potential target gene locus, named Bra2Ms, might be located on A2. Based on 10 of the 22 SNPs, allele-specific-polymerase chain reaction (AS-PCR) primers and single sequence repeat (SSR) primers were designed, 5 AS-PCR primers and 9 SSR primers showed difference between the bulks in electrophoretic determination. Analysis of these markers in F2 population revealed that Bra2Ms was genetically delimited to a region of 1.2 cM. We also detected two co-segregated markers SSRa2-951 and SSRa2-960 in this region. The markers identified in our study might facilitate the transfer of recessive genic male sterility alleles to other favorable genetic backgrounds. Furthermore, these markers will support a map-based clone of Bra2Ms.

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Acknowledgments

This study was supported by the Innovation Fund of Wuhan Academy of Agricultural Science and Technology (BSH201401), the basic research Project of Wuhan Science and Technology Bureau (2015020101010077), the National Science-technology Support Plan Project in the twelfth five year (2012BAD02B01-17).

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Authors and Affiliations

  1. Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, 430345, People’s Republic of China

    Xia Li, Aihua Wang, Zhenhua Hu, Jiazao Lin & Guolin Zhou

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xia Li & Jinxing Tu

  3. Industrial Crop Research Institute, Yunnan Academy of Agricultural Science, Kunming, 650223, People’s Republic of China

    Feng Zu

Authors
  1. Xia Li
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  2. Aihua Wang
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  3. Feng Zu
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  4. Zhenhua Hu
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  5. Jiazao Lin
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  6. Guolin Zhou
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  7. Jinxing Tu
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Correspondence to Guolin Zhou.

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Author Feng Zu declares that he has no conflict of interest. Author Jinxing Tu declares that he has no conflict of interest. Author Xia Li declares that she has no conflict of interest. Author Aihua Wang declares that she has no conflict of interest. Author Zhenhua declares that he has no conflict of interest. Author Jiazao Lin declares that he has no conflict of interest. Author Guolin Zhou declares that he has no conflict of interest.

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Li, X., Wang, A., Zu, F. et al. Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in non-heading Chinese cabbage. Genes Genom 38, 1151–1157 (2016). https://doi.org/10.1007/s13258-016-0459-6

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  • DOI: https://doi.org/10.1007/s13258-016-0459-6

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