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Characterization of Physa acuta expressed sequence tags and transcript mining following cadmium exposure

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An Erratum to this article was published on 22 October 2015

Abstract

Freshwater molluscs have found strategic applications in public and veterinary health. Additionally, they have been used as bioindicator organisms towards environmental biomonitoring of polluted waters. The freshwater pulmonate, Physa acuta has been extensively studied as a potential biomarker in aquatic habitats with heavy metal, chemical, and microbial stressors. Lack of genomic resources is the bottleneck towards the study of candidate genes responsible for the unique adaptation of the mollusc to contaminated aquatic habitats. We have reported an EST survey of functionally relevant stress and defense related genes from P. acuta in our earlier study. In continuation of the same, we generated 1108 high-quality ESTs from a normalized cDNA library, pooled from the RNA isolated from the whole body tissue of CdCl2 exposed specimens of P. acuta. After clustering and assembly, we finally obtained 730 unique sequences representing 114 contigs and 616 singletons. Annotation of EST sequences revealed that 65.2, 62.8, and 35.9 % show significant homology to NCBI non-redundant database, Molluscs amino acid database, and NCBI KOG database, respectively. The functional characterization of the ESTs in Cd treated P. acuta group showed a greater proportion of signal transduction, cytoskeleton, and extracellular structure relevant genes compared with the control group. A large proportion of transcripts of cadmium treated P. acuta fell under the poorly characterized group of genes. These EST resources provide valuable information on Cd-specific transcript expression of P. acuta and could be utilized by scientists for developing new biomonitoring markers.

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Acknowledgments

This research was supported by Soonchunhyang University Research Funding (20130638) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2009-0073975).

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    Authors and Affiliations

    1. Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do, 336-745, Republic of Korea

      Bharat Bhusan Patnaik, Se Won Kang, Hee-Ju Hwang, So Young Park, Tae Hun Wang, Eun Bi Park & Yong Seok Lee

    2. Department of Applied Biology, College of Natural Resources and Life Science, Dong-A University, 550-37 Nakdongdaero, Sahagu, Busan, 604-714, Republic of Korea

      Ji Eun Jeong

    3. Trident School of Biotech Sciences, Trident Academy of Creative Technology (TACT), Bhubaneswar, Odisha, 751024, India

      Bharat Bhusan Patnaik

    4. Korea Zoonosis Research Institute, Chonbuk National University, 567 Baekje-daero, deokjin-gu, Jeonju-si, Jeollabuk-do, 561-756, Republic of Korea

      Jae Bong Lee

    5. East Sea Fisheries Research Institute, NFRDI, Gangneung, 210-861, Republic of Korea

      Myung-Mo Nam

    6. College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-Dong, Buk-gu, Gwangju, 500-757, Republic of Korea

      Yong Hun Jo & Yeon Soo Han

    7. Institute of Environmental Research, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 200-701, Republic of Korea

      Jun-Sang Lee

    8. Research Institute, GnC BIO Co., Ltd., 621-6 Banseok-dong, Yuseong-gu, Daejeon, 305-150, Republic of Korea

      Hong Seog Park

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    1. Ji Eun Jeong
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    Correspondence to Yong Seok Lee.

    Ethics declarations

    The tissue sampling for experiments was conducted in accordance with the International Guiding Principles for Biomedical Research involving animals (1985) (http://www.ncbi.nlm.nih.gov/books/NBK25438/).

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    The authors declare that they have no conflict of interest.

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    Ji Eun Jeong, Bharat Bhusan Patnaik and Yong Seok Lee contributed equally.

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    Jeong, J.E., Patnaik, B.B., Kang, S.W. et al. Characterization of Physa acuta expressed sequence tags and transcript mining following cadmium exposure. Genes Genom 37, 1017–1025 (2015). https://doi.org/10.1007/s13258-015-0334-x

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