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MicroRNA expression profiles in conventional and micropropagated Dendrobium officinale

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Abstract

MicroRNA (miRNA) is defined as an endogenous ~22 nt noncoding RNA that has critical gene-regulatory functions in plants. Dendrobium officinale is among the most important herbs in traditional Chinese medicine. However, no miRNA of D. officinale has been reported to date. To identify the miRNA in D. officinale and to detect the differentially expressed miRNA between conventional and micropropagated D. officinale plants, miRNA microarray was performed following quantitative real-time RT-PCR validation. A total of 120 miRNAs from 37 miRNA families were identified in both conventional and micropropagated D. officinale plants. Among the total number of miRNAs, 45 miRNAs from six families were differentially expressed, including five up-regulated families (miR156, miR164, miR171, miR827 and miR529) and one down-regulated family (miR167) in micropropagated group. Web microrna designer and gene ontology term-enrichment analyses were applied for prediction and functional analysis of the target genes of differentially expressed miRNA. Such miRNAs are mainly related to hormone stimulus response, biological and metabolic process regulation, formation of intracellular organelle, and nucleic acid binding.

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Acknowledgments

This research was supported by the National Natural Science Fund of China (No. 30772712) and Zhejiang Open Foundation of the Most Important Subjects.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Lab of Natural & Traditional Medicines, Institute of Bioengineering, College of Life Sciences, Zhejiang Sci-Tech University, No. 928, St. 2, Xiasha, Hangzhou, 310018, China

    Zhili Yang, Dongfeng Yang, Xianfeng Ding, Yong Gao, Donghai Li & Tao Xu

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  1. Zhili Yang
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  2. Dongfeng Yang
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  3. Xianfeng Ding
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  4. Yong Gao
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  5. Donghai Li
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Correspondence to Tao Xu.

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Yang, Z., Yang, D., Ding, X. et al. MicroRNA expression profiles in conventional and micropropagated Dendrobium officinale . Genes Genom 37, 315–325 (2015). https://doi.org/10.1007/s13258-014-0257-y

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