Abstract
Experiments using multi-step protocols often involve several restrictions on the randomization. For a specific application to in vitro testing on microplates, a design was required with both a split-plot and a strip-plot structure. On top of two-level treatment factors and the factors that define the randomization restrictions, a multi-level fixed blocking factor not involving further restrictions on the randomization had to be added. We develop a step-by-step approach to construct a design for the microplate experiment and analyze a response. To consolidate the approach, we study various alternative scenarios for the experiment.Supplementary materials accompanying this paper appear online
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This research was financially supported by the Fonds Wetenschappelijk Onderzoek (FWO, Flanders, Belgium).
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Bohyn, A., Schoen, E.D., Ng, C.P. et al. Design and Analysis of a Microplate Assay in the Presence of Multiple Restrictions on the Randomization. JABES (2023). https://doi.org/10.1007/s13253-023-00570-1
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DOI: https://doi.org/10.1007/s13253-023-00570-1