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Development of a new advanced animal cradle for small animal multiple imaging modalities: acquisition and evaluation of high-throughput multiple-mouse imaging

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Abstract

The physiological conditions of small animals are an essential component to be considered when acquiring images for pre-clinical studies, and they play a vital role in the overall results of a study. However, several previous studies did not consider these conditions. In this study, a new animal cradle that can be modified and adjusted to suit multiple imaging modalities such as positron emission tomography (PET)/computed tomography (CT) and magnetic resonance imaging (MRI) was developed. Unlike previous cradles where only one mouse can be imaged at a time, a total of four mice can be imaged simultaneously using this new cradle. Additionally, fusion images with high-throughput multiple-mouse imaging (MMI) of PET/MRI and PET/CT images can be acquired using this newly developed cradle. The dynamic brain images were also acquired simultaneously by applying PET dynamic imaging technology to high-throughput MMI methods. The results of this study suggest that the newly developed small animal cradle can be widely used in pre-clinical studies.

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Acknowledgements

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (2018R1A2B2005343).

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Authors and Affiliations

  1. Department of Biomedical Engineering, College of Medicine, Research Institute of Biomedical Engineering, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea

    Hunnyun Kim, Chi-Hyeon Yoo & Bo-Young Choe

  2. Animal Research and Molecular Imaging Center, Samsung Medical Center, 81, Irwon-ro, Gangnam-gu, Seoul, 06351, Republic of Korea

    Hunnyun Kim, Geun Ho Im & Yeup Yoon

  3. Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology Sungkyunkwan University, 81, Irwon-ro, Gangnam-gu, Seoul, 06351, Republic of Korea

    Yeup Yoon

  4. Department of Radiological Science, Shinhan University, 95, Hoam-ro, Uijeongbu-si, Gyeonggi-do, 11644, Republic of Korea

    Ho-Sung Kim

  5. Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 149, 13th St, Charlestown, MA, 02129, USA

    Chi-Hyeon Yoo

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  1. Hunnyun Kim
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  2. Geun Ho Im
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Correspondence to Bo-Young Choe.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Kim, H., Im, G.H., Yoon, Y. et al. Development of a new advanced animal cradle for small animal multiple imaging modalities: acquisition and evaluation of high-throughput multiple-mouse imaging. Phys Eng Sci Med 44, 1367–1376 (2021). https://doi.org/10.1007/s13246-021-01065-4

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  • DOI: https://doi.org/10.1007/s13246-021-01065-4

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