Identification of serotonin 2A receptor as a novel HCV entry factor by a chemical biology strategy
Hepatitis C virus (HCV) is a leading cause of liver disease worldwide. Although several HCV protease/polymerase inhibitors were recently approved by U.S. FDA, the combination of antivirals targeting multiple processes of HCV lifecycle would optimize anti-HCV therapy and against potential drug-resistance. Viral entry is an essential target step for antiviral development, but FDA-approved HCV entry inhibitor remains exclusive. Here we identify serotonin 2A receptor (5-HT2AR) is a HCV entry factor amendable to therapeutic intervention by a chemical biology strategy. The silencing of 5-HT2AR and clinically available 5-HT2AR antagonist suppress cell culture-derived HCV (HCVcc) in different liver cells and primary human hepatocytes at late endocytosis process. The mechanism is related to regulate the correct plasma membrane localization of claudin 1 (CLDN1). Moreover, phenoxybenzamine (PBZ), an FDA-approved 5-HT2AR antagonist, inhibits all major HCV genotypes in vitro and displays synergy in combination with clinical used anti-HCV drugs. The impact of PBZ on HCV genotype 2a is documented in immune-competent humanized transgenic mice. Our results not only expand the understanding of HCV entry, but also present a promising target for the invention of HCV entry inhibitor.
KeywordsHCV serotonin 2A receptor entry antiviral drug
Hepatitis C virus (HCV) is a major health threat affecting more than 170 million people worldwide (Webster et al., 2015). Approximately 80% of infections persist to chronicity and lead to liver pathologies, including fibrosis, cirrhosis and hepatocellular carcinoma. Several host factors have been identified to facilitate multistep HCV entry, including CD81 (Pileri et al., 1998), scavenger receptor class B type 1 (SR-BI) (Scarselli et al., 2002), claudin 1 (CLDN1) (Evans et al., 2007), occludin (OCLN) (Ploss et al., 2009), the low-density lipoprotein receptor (LDLR) (Agnello et al., 1999), liver/lymph node-specific intercellular adhesion molecule 3-grabbing integrin (Gardner et al., 2003; Lozach et al., 2003) and glycosaminoglycans (Germi et al., 2002), cholesterol uptake receptor Niemann-Pick C1-like 1 (NPC1L1) (Sainz et al., 2012), receptor tyrosine kinases epidermal growth factor receptor and ephrin receptor A2 (Lupberger et al., 2011), transferrin receptor 1 (TfR-1) (Martin and Uprichard, 2013), serum response factor binding protein 1 (SRFBP1) (Gerold et al., 2015) and very-low-density lipoprotein receptor (VLDLR) (Ujino et al., 2016).
Since HCV was first discovered in 1989, extensive efforts have been launched for anti-HCV therapy intervention. Upon pegylated interferon (PEG-IFN) and ribavirin as the standard therapy for a long time, several direct-acting antivirals (DAAs) have been approved by FDA for anti-HCV therapy in combination with PEG-IFN and ribavirin, leading to improvement of viral clearance rate in patients (Ozaras et al., 2013). However, these DAAs have a low genetic barrier to resistance, side effects, potential for drug–drug interaction, and are costly. Very recently, the risk of hepatitis B virus (HBV) reactivating in some patients treated with DAAs for hepatitis C has been warned (Communication FDS, 2016; De Monte et al., 2016; Wang et al., 2017). Therefore, there remains a great need to improve HCV therapy, analogous to HIV therapy, with combination of antivirals targeting multiple aspects of HCV lifecycle. HCV entry is an essential target step for antiviral development, but FDA-approved HCV entry inhibitor remains exclusive. Most interestingly, from a chemical biology aspect, new antiviral compounds targeting host proteins can be used as chemical probes to provide new vision for uncovering host factors in HCV lifecycle, furthering the understanding of HCV biology.
The antagonist of 5-HT2A receptor inhibits HCV proliferation
Adrenergic receptor family has five major members, α1, α2, β1, β2 and β3 receptors (Bang and Choi, 2015). And the 7 general 5-HT receptor classes include a total of 14 known 5-HT receptors, including 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT4, 5-HT5A, 5-HT5B, 5-HT6 and 5-HT7 receptors (Barnes and Sharp, 1999). We first selected other selective antagonists to assess which GPCRs antagonism of PBZ is responsible for HCV inhibition. Alfuzosin (a selective α1AR antagonist) (Wilde et al., 1993), atipamezole (a selective α2AR antagonist) (Vacher et al., 2010), and acebutolol (a βAR antagonist) (van den Meiracker et al., 1988), atenolol (a selective β1AR antagonist) (Baldwin et al., 1986) and (S)-timolol maleate (a β1AR and β2AR antagonist) (Weinstock et al., 1976) present no inhibition on HCV proliferation, suggesting HCV inhibition of PBZ is not correlated to its antagonism on adrenergic receptors (Figs. 1A, 1B and S1A). Similarly, agomelatine (a 5-HT2B and 5-HT2C receptors antagonist) (Popoli, 2009), azasetron (a selective 5-HT3 receptor antagonists) (Tsukagoshi, 1999), piboserod (a selective 5-HT4 receptor antagonist) (Brattelid et al., 2004), SB742457 (a selective 5-HT6 receptor antagonist) (Upton et al., 2008) and SB269970 (a selective 5-HT7 receptor antagonist) (Mahe et al., 2004) do not display HCV inhibition till 20 μmol/L (Figs. 1A, 1C and S1B). SB699551, which is a selective 5-HT5A receptor antagonist (Corbett et al., 2005), also did not present anti-HCV activity at the concentration with no cytotoxicity (Fig. S1C). Together hints the antagonism of 5-HT2AR by PBZ is responsible for HCV inhibition.
5-HT2A receptor functions in HCV life cycle
Similar to what has been observed for other HCV receptors or entry factors (Sainz et al., 2012), we observed down-regulation of 5-HT2AR in HCVcc-infected Huh7.5.1 cells. The expression of 5-HT2AR protein were remarkably down-regulated within 6 days of HCV infection, but were moderately regained from 8 day infection (Fig. 2B). Because of the correlation between 5-HT2AR expression and HCV infection, we next assessed whether 5-HT2AR expression affects HCV infection by transfecting Huh7.5.1 cells with shRNAs targeting 5-HT2AR or CD81. Compared to cells transfected with an irrelevant shRNA (sh-NC), CD81- and 5-HT2AR-silenced cells were significantly less susceptible to HCVcc up to 120 h post-transfection (Fig. 2C). Inhibition of HCV also correlates with a reduction in 5-HT2AR expression (Fig. 2D). As sh-5HT2AR-3 displays the best silencing and inhibition efficiency, we used this shRNA in the following experiment and referred it as sh-5HT2AR hereafter. Moreover, the inhibition is 5-HT2AR specific, since the silencing of 5-HT2AR does not result off-target effects on the transcriptions of other known HCV receptors or entry factors (Fig. S3). Furthermore, the inhibition is HCV specific, as the treatment of PBZ and the silencing of 5-HT2AR had no effect on vesicular stomatitis virus G protein pseudo-typed virus (VSV-Gpp) (Figs. 2D and S4A). Lastly, the effects of the silencing of endogenous 5-HT2AR on HCVcc infection are rescued by the RNAi-resistant ectopic expression of wild-type 5-HT2AR (Fig. 2E). And the overexpression of 5-HT2AR enhances HCV infection (Fig. S5).
We next assessed whether HCV infection was sensitive to inhibition by antibody-mediated blocking of cell surface 5-HT2AR. Huh7.5.1 cells were treated with antibodies against 5-HT2AR NTD, ECL1 and ECL2 before HCV infection. Because the antibody against ECL3 is not available, we used its blocking peptide to test the impact on HCV infection. We observed that the reagent targeting NTD, but not ECL1, ECL2 or ECL3, suppressed HCV infection (Fig. 2F). Conceivably, the inhibition of antibody was HCV specific, as it had no effect on VSV-Gpp proliferation (Fig. S4B).
To assess whether the function of 5-HT2AR in HCV lifecycle is a general observation but is not cell-specific, we further determined the HCV inhibition of PBZ and the silencing of 5-HT2AR in Huh7 cells and PHHs. Similar to those have been observed in Huh7.5.1 cells, PBZ treatment and the down-regulation of 5-HT2AR inhibited HCV in Huh7 cells and PHHs (Fig. 2G and 2H). Moreover, we observed PBZ sensitivity across a panel of JFH-1 based inter-genotypic HCV clones containing the structural regions of diverse HCV genotypes (1–6) (Fig. 2I).
We next assessed whether 5-HT2AR is required for HCV cell-to-cell spread following a reported foci spread assay (Martin and Uprichard, 2013). A focus containing 4 or more GFP-positive cells is taken as evidence of cell-to-cell spread, as a focus containing fewer than 4 cells could result from cell division during the assay (Martin and Uprichard, 2013). The results showed that approximate 80% of the HCV foci contain 4 or more GFP-positive cells in sh-NC-containing cells or the cells treated by anti-IgG mAb, while less than 10% of the foci contain 4 or more GFP-positive cells in the CD81-silenced cells or in the cells treated with anti-CD81 mAb (Fig. 2J). In the 5-HT2AR-silenced cells or the cells treated by PBZ, over 80% of the HCV foci contain less than 4 GFP-positive cells, supporting that 5-HT2AR function affects HCV cell-to-cell spread (Fig. 2J).
5-HT2A receptor facilitates HCV late endocytosis event
We next assessed the entry step that 5-HT2AR works on through time-of-addition analysis (Fig. 3C). Huh7.5.1 cells were infected with HCVcc at 4 °C for 2 h (T = − 2 h). After removing unbound viruses, cells were incubated at 37 °C (T = 0 h), and reagents were added to the infected cells at different time points. We selected heparin, anti-CD81 antibody and bafilomycin A1 as controls to represent the typical HCV entry inhibitors working on initial attachment, attachment and the early entry process, and late entry events, respectively (Evans et al., 2007; Liu et al., 2012). HCV is mostly sensitive to bafilomycin A1 at 0–2 h after the 37 °C temperature shift and the HCV inhibiting effects of anti-CD81 antibody decreases at the time of the temperature shift, which is consistent with previous reports (Liu et al., 2012). The growth curve of HCV in the presence of PBZ and bafilomycin A1 is similar, indicating that PBZ acts on the late entry process.
We further verified whether PBZ blocks infection at the initial attachment step or a post-attachment event. PBZ, heparin, bafilomycin A1 and anti-CD81 antibody were either supplied together with HCVcc to cells during the 4 °C attachment step only, and then removed prior to shifting the temperature to 37 °C; or provided only after the temperature shift. The results reveal that all of four reagents suppress HCV infection if they are present throughout the course of the experiment (Fig. 3D). Inhibition by heparin is predominant when it is added during the 4 °C attachment step, whereas bafilomycin A1 is most effective during the post-attachment stage. These are consistent with previous reports (Evans et al., 2007). Again, similar to bafilomycin A1, PBZ strongly inhibits viral infection at post-attachment. Finally, by using heparin and NH4Cl (an inhibitor of endosomal acidification and HCV membrane fusion (Sainz et al., 2012) as controls, we observed that PBZ, as well as NH4Cl, does not block the binding of HCV virions to host cells, which is in a sharp contrast with heparin (Fig. 3E). And as expected, the silencing of 5-HT2AR only negligibly affects the attachment of HCV to cells (Fig. 3F).
We next used a reported fluorescence-based HCV fusion assay (Sainz et al., 2012) to dissect whether 5-HT2AR acts at or before membrane fusion. HCVcc was labeled with the hydrophobic fluorophore DiD, which can be anchored on the lipid membrane and will be self-quenching at high concentrations. When viral entry occurs, DiD diffuse away from each other, causing dequenching and allowing for the monitor of virus membrane fusion. The result shows that PBZ inhibits HCVccDiD fusion as a same level compared to NH4Cl till 12 h after binding (Fig. 3G). All these results demonstrated that the antagonism of 5-HT2AR inhibits HCV cell entry in the late endocytosis stage at or before virus membrane fusion.
Antagonism of 5-HT2AR reorganize the cellular distribution of CLDN1
We are interested to assess the working mechanism of 5-HT2AR in HCV lifecycle. We first hypothesized that 5-HT2AR may function as a co-receptor, analogous to CCR5/CXCR4 in HIV entry, and attach HCV virion through the interaction with viral envelop protein(s). But no convinced interaction of 5-HT2AR with HCV virion or E1/E2 protein can be detected (data not shown).
A recent work reported that cAMP-dependent protein kinase A (PKA) phosphorylation of CLDN-1 play a role in the cellular distribution of CLDN1 (French et al., 2009), and 5-HT2AR is also known to be associated with cAMP/PKA pathway stimulation (Berg et al., 1994; Tamir et al., 1992; Raymond et al., 2001). We next assessed whether 5-HT2AR alters the cellular localization of CLDN1 via PKA-dependent phosphorylation of CLDN1. The phosphorylation of CLDN1 in PBZ-treated and 5-HT2AR-silenced cells are significantly lower than that in control cells, and is remarkably up-regulated in 5-HT2AR-overexpressed cells (Fig. 4H). Moreover, the treatment of H89, an inhibitor of PKA, suppresses the membrane localization reinforcement of CLDN1 by the overexpression of 5-HT2AR (Fig. 4I). Notably, the signaling of 5-HT receptors are very complicated and 5-HT2AR is also known to regulate many other downstream signaling pathways (Raymond et al., 2001). Whether other 5-HT2AR-associated downstream pathway would be involved in HCV lifecycle needs further investigation. Nevertheless, all these results show that the function of 5-HT2AR is necessary for the phosphorylation and the correct membrane distribution of CLDN1, thus ensuring HCV natural proliferation.
Synergistic antiviral effect of PBZ in combination with anti-HCV drugs
PBZ works in vivo
Using a chemical biology strategy, we have demonstrated that 5-HT2AR is a HCV entry factor that functions in the late endocytosis stage at or before membrane fusion. To our best knowledge, this is the first time to dissect the role of a GPCR in HCV lifecycle. Notably, CLDN-1 is a HCV entry factor and confers HCVcc and HCVpp entry. CLDN1 does not directly interact with HCV glycoproteins and contributes to the post-binding steps of HCV entry by interacting with CD81 (Evans et al., 2007; Harris et al., 2010; Lindenbach and Rice, 2013). Because 5-HT2AR plays a role in the correct plasma membrane localization of CLDN-1 for HCV entry, the impacts to HCVcc and HCVpp, no influence on HCV attachment, and function in HCV late endocytosis of PBZ treatment and 5-HT2AR silencing are well fit with reported observations of CLDN1 in HCV lifecycle. Recently, another GPCR antagonist chlorcyclizine (CCZ), a first-generation of H1-antihistamines, was reported to potently inhibit late-stage HCVcc entry, while its action mode is yet clear (He et al., 2015). A significant discrepancy on anti-HCV activity of 5-HT2AR antagonist and antihistamines is that PBZ inhibits both HCVcc and HCVpp, but CCZ exhibits no inhibitory effect on HCVpp (He et al., 2015). The dual-inhibition on HCVcc and HCVpp of PBZ is consistent with the crucial role of CLDN1 in HCV entry, and the precise working mode of antihistamines on HCV is warranted further investigation. Lastly, though in vivo synergistic anti-HCV activity remains to be determined, the strong synergistic effect of of PBZ in the combination with currently used anti-HCV drugs will probably provide a great potential for the optimal anti-HCV therapy.
MATERIALS AND METHODS
Huh7.5.1 cells were gifted by Apath L.L.C. HEK 293T cells were purchased from the American Type Culture Collection (ATCC) (Manassas, USA). Huh7 cells were gifted by Dr. Wang J. from Peking University. Primary human hepatocytes (PHHs) were purchased from Wuhan Procell Corporation (China). All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (catalog number 10099-141) (GIBCO, USA), 100 U/mL penicillin, 100 mg/mL streptomycin, and 2 mmol/L l-glutamine at 37 °C in a humidified incubator with 5% CO2.
Reagents and antibodies
Alfuzosin hydrochloride (catalog number HY-B0192A), atipamezole hydrochloride (catalog number HY-12380), acebutolol hydrochloride (catalog number HY-17497A), atenolol (catalog number HY-17498), (S)-timolol maleate (catalog number HY-17380), agomelatine (catalog number HY-17038), azasetron hydrochloride (catalog number HY-B0068), piboserod (catalog number HY-15574), SB742457 (catalog number HY-14339), agomelatine (catalog number HY-17038), phenoxybenzamine hydrochloride (catalog number HY-B0431A) and SB269970 (catalog number HY-15370) were purchased from MedChemExpress (USA). SB699551 (catalog number SML0853) was purchased from Sigma (USA). The compounds were initially dissolved in 95% DMSO to a concentration of 10 mmol/L, and the stock solutions were stored at −20 °C. Recombinant human IFNα-2b was purchased from GenScript (China). Heparin sodium was purchased from Rui Taibio (China). Bafilomycin A1 (catalog number IIC2501-0001MG) was purchased from Gene Operation (China). Immediately before addition, these reagents were diluted to the desired concentrations using DMEM (GIBCO, USA) with 10% FBS (GIBCO, USA). The Vybrant® DiD cell-labeling solution (catalog number V22887), which has an excitation wavelength of 644 nm and an emission wavelength of 665 nm, was purchased from Invitrogen (USA). Lipofectamine 2000, lipofectamine 3000 and SuperScript III First-strand Synthesis System for RT-PCR kits were purchased from Invitrogen (USA). The MEGAscript T7 High Yield Transcription kit was purchased from Ambion (USA). The HiScript® II One Step qRT-PCR SYBR® Green Kit (Q221-01) and RNA isolater Total RNA Extraction Reagent (R401-01) were purchased from Vazyme (China). The WST-1 reagent for cell viability assays was purchased from Roche (Swiss). The ATP Determination Kit (catalog number A22066) was purchased from Invitrogen (USA). The ToxiLight™ bioassay kit (catalog number LT07-217) was purchased from LONZA (USA). Fast Mutagenesis System (catalog number FM111-01) was purchased from Transgen Biotech (China).
A monoclonal mouse antibody against the N-terminus of human 5-HT2AR (catalog number SC-166775) (Santa Cruz Biotechnology, USA) was used in Western blot detection, immunofluorescence and HCV neutralization. A mouse monoclonal antibody C7-50 against HCV core (catalog number ab2740) was purchased from Abcam (USA). Anti-GAPDH monoclonal antibody (catalog number L018) was purchased from Transgen Biotech (China). Anti-CLDN1 C-Terminal antibody (SAB4503545) for WB and monoclonal antibody (SAB5500083) for flow cytometry and immunofluorescence were purchased from Sigma (USA). The anti-CD81 monoclonal antibody JS81 (catalog number 555675) was purchased from BD Biosciences (USA). The secondary antibodies HRP-conjugated goat anti-mouse IgG (H + L) (catalog number J092) for Western blot analysis were purchased from Transgen Biotech (China), and AlexaFluor 488-labeled goat anti-mouse IgG (H + L) (catalog number A0428) for immunofluorescence were purchased from Beyotime (China). The rabbit polyclonal antibodies to human 5-HT2AR ECL1 and ECL2 were obtained from GenScript (China). The rabbit immunogen is peptide-KLH conjugated, and the polyclonal antibodies were purified by sterile filtration affinity purification. The blocking peptide of human 5-HT2AR ECL3 (catalog number LS-E26707) was purchased from LifeSpan BioSciences Inc (USA).
Generation of HCVcc and HCVrep
HCV JFH-1 virus with a luciferase reporter gene (hRluc-JFH1) was constructed according to a previously described method with modifications (Sainz et al., 2012; Qing et al., 2016; Han et al., 2009). Based on the pJFH-1 plasmid gifted by Apath L.L.C., a humanized Renilla luciferase reporter gene was inserted between amino acids 399 and 400 of NS5A in the HCV JFH-1 genome containing four adaptive mutations (core-K78E, E2-G451R, NS3-M260K and NS5A-T462I). The plasmids harboring HCV various subtypes such as 1a (TNCC), 1b (Con1), 2a (JC-1), 2b (J8cc), 3a (S52), 4a (ED43), 5a (SA13) and 6a (HK) were kindly gifts from Pro. Jens Bukh. These plasmids harboring HCV genomes were made through digestion with the XbaI restriction enzyme and used as a template for RNA transcription. The transcripts were prepared in vitro using the Ambion MEGAscript Kits, and then 10 μg RNA was mixed with 400 μL Huh7.5.1 cells at a concentration of 1 × 107 cells/mL. After electroporation, Huh7.5.1 cells containing viral transcripts were seeded in 10 cm dishes. The cells were cultured and passaged every 3 days until they showed significant apoptosis, and the supernatant was collected and filtered to obtain a stock solution of hRluc-JFH-1 virus. HCV JFH-1 virus with an EGFP reporter gene is an infectious HCV monocistronic reporter virus constructed by inserting an EGFP reporter gene between amino acids 399 and 400 of NS5A of the HCV JFH-1 genome (Han et al. 2009). A stable Huh7.5.1 cell line containing a JFH-1 subgenomic replicon (HCVrep) was created following previously reported methods (Hao et al., 2007; Lohmann et al., 1999). The stable replicon-containing cells were selected and maintained in medium containing 1 mg/mL G418 geneticin (Invitrogen, USA).
Generation of HCVpp and VSV-Gpp
Pseudotyped viral particles of HCV or VSV were generated by co-transfection of the JFH1 full-length envelope-expressing plasmid (pCDN3.1-JFH1-cE1E2) or the vesicular stomatitis virus glycoprotein (pCDN3.1-VSVG) together with the pNL4-3R-E-luciferase backbone construct into HEK 293T cells as described previously (Cui et al., 2013; Shukla et al., 2010). At 48 h post-transfection, the culture supernatant was harvested, clarified, filtered through 0.45 μm filters (Millipore, USA) and tested for luciferase activity to standardize the viral input in the subsequent inhibition analysis.
To obtain the titers of viruses containing luciferase reporter genes, the virus stocks were serially diluted 1:10 and incubated with Huh7.5.1 cells for 48 h at 37 °C. The cells were then harvested, and the luminescence was detected according to the manufacturer’s protocol of the Renilla-Glo Luciferase or Bright-Glo Luciferase Assay System. The titers of wild-type HCVcc or that containing an EGFP reporter gene were determined by endpoint dilution assays (EPDA) using focus-forming units (ffu) (Zhong et al., 2006). Huh7.5.1 cells (1 × 104) were seeded per well in 96-well microtiter plates. After an overnight culture, the viruses were serially diluted 10-fold with DMEM containing 10% FBS (10−1 to 10−8-fold dilutions) and used to infect Huh7.5.1 cells. The plates were then incubated at 37 °C in 5% CO2. For viruses containing EGFP reporter genes, the EGFP expression levels were observed using an epifluorescence microscope after 3 to 4 days. For wild-type virus, an antibody against the HCV core protein was used to mark the virus. The determination of the virus titer was shown as the 50% tissue culture infectious dose (TCID50). The multiplicity of infection (MOI) is the average number of viruses (TCID50) per cell.
Virus inhibition assay
Cells or cells containing an HCV (JFH-1) subgenomic replicon were seeded in 96-well plates at a density of 1 × 104 cells per well and cultured at 37 °C overnight. The initial concentration of the compounds was 20 μmol/L and serial dilutions were made. For HCVcc, HCVpp or VSV-Gpp, the serially diluted compounds were mixed with a certain titer of virus and added to different cells. For the inhibition of HCVrep, the serially diluted compounds were mixed with the stable Huh7.5.1 cell line containing a subgenomic replicon, followed by 2 days of culture at 37 °C. The amounts of virus were detected by the luminescence or qRT-PCR. EC50 is the concentration of the compound at which the luminescence or HCV RNA levels is reduced by 50%. The EC50 values were calculated using GraphPad Prism 5.0 software.
Cell viability assay
Cells were seeded in 96-well plates at a density of 1 × 104 cells per well and cultured at 37 °C overnight. The cells were incubated with the serially diluted compounds or proteins for 48 h. The cell viability was determined in 96-well tissue culture plates using the cell proliferation reagent WST-1 (Roche, Swiss), and the absorbance (OD450/OD630) was measured to detect the cytotoxicity of compounds according to the manufacturer’s protocol.
The human 5-HT2AR-pUC57 gene (GenBank: NM_000621.4) was obtained from Genscript (China). The 5-HT2AR gene was amplified and cloned with the primers 5HT2AR-F and 5HT2AR-R as a BamHI/Xhol fragment into BamHI/Xhol—digested pcDNA3.1 for overexpression.
RNA interference and infections
The shRNAs targeting human 5-HT2AR (pGPH1/GFP/Neo-HTR2A-Homo-1, pGPH1/GFP/Neo-HTR2A-Homo-2, pGPH1/GFP/Neo-HTR2A-Homo-3, pGPH1/GFP/Neo-HTR2A-Homo-4) were purchased from GenePharma (China). Cells were seeded in 24-well plates at a density of 1 × 105 cells per well. After culture at 37 °C overnight, cells were transfected with sh-5HT2AR or sh-NC plasmids by lipofectamin 2000 as per the manufacturer’s instructions. After 48 h, the cells were incubated in selection medium containing 1 mg/mL G418 geneticin. After 24 h, cultures were either mock-inoculated or inoculated with HCVcc at indicated times post-transfection. Total cellular RNA was harvested for extraction in RNA isolater Total RNA Extraction Reagent as the manufacturer’s instructions for qRT-PCR analysis.
Silencing of endogenous expression of 5-HT2AR
The shRNA recombinant lentiviruses targeting human 5-HT2AR and CD81 were purchased from Shanghai GenePharma (China), and the virus titers were determined to be 1 × 108 TU/mL. The shRNA recombinant lentiviruses were incubated with 5 μg/mL polybrene to enhance the lentivirus infection and added to PHHs at an MOI of 5. For the knockdown cell lines, PHHs were incubated in selection medium containing 5 μg/mL puromycin (Invitrogen, USA) beginning at 24 hpi. The mRNA levels of the target proteins in knockdown cells were confirmed at 96 hpi by qRT-PCR. The knockdown cell lines were stable after about five days of cell culture and were then used for experiments.
The production of highly infectious and DiD-labeled HCV virions
The supernatants of wild type HCV (JFH-1) virus cultures were collected, centrifuged to remove cell debris, filtered through 0.45 μm filters (Millipore, USA). Viral supernatants were concentrated by PEG precipitation. Briefly, 40 mL filtered viral supernatants were incubated overnight with 8% (w/v) PEG followed by centrifugation (8,000 ×g, 20 min) and resuspended in 10 mL of spent supernatant. Virus was centrifuged (8,000 ×g, 15 min) and resuspended in a final volume of 1 mL of cell culture DMEM. Virus was labeled by adding 5 μL (5 μmol/L final concentration) of lipophilic dye DiD. Virus and dye were incubated for 1 h with shaking at 37 °C while protected from light with shaking. To remove the unbound DiD, the labeled viruses were buffer-exchanged and concentrated to 200 μL in PBS by ultra-filtration with a MW cutoff of 100 kDa three times, with 20 mL PBS added each time. The labeled viruses were collected and subjected to viral titration assays using focus formation assays or RNA measurements by qRT-PCR. The concentrated DiD-labeled HCV was used for subsequent fusion studies.
HCVccDiD fusion assay
Huh7.5.1 cells were seeded per well in 48-well microtiter plates. After an overnight culture, cells were treated for approximately 2 h with indicated concentrations of 0.5% DMSO, NH4Cl and PBZ at 37 °C. Cells were incubated on ice for 10 min before the addition of DiD-HCV (MOI = 1). For DiD dequenching analysis, vehicle-treated or treated cultures were mock-infected with cDMEMDiD or infected with HCVccDiD JFH-1 at an MOI of 5 for 1 h at 4 °C. After washing to remove unbound viral particles, plates were brought to 37 °C and immediately placed in a Fluostar OPTIMA microplate reader (Thermofisher, USA) and DiD dequenching was measured every 6 min over the course of 12 h at 37 °C at 640 nm (excitation) and 670 nm (emission) for 120 cycles in kinetic mode.
HCV cell-to-cell spread was measured according to a previously described method, with modifications (Gondar et al., 2015). Huh 7.5.1 cells were seeded in 24-well plates at a density of 1 × 105 cells per well. After culture at 37 °C overnight, Huh 7.5.1 cells were infected with HCV JFH-1 virus with an EGFP reporter at an MOI of 0.01. For shRNA-containing Huh7.5.1 cells, at 24 hpi, the cells were washed and incubated in fresh medium containing 1% methyl cellulose (Sigma, USA), which prevents cell-free viral spread. For Huh7.5.1 cells, at 24 hpi, antibodies against CD81 or an isotype control antibody were added to the culture medium together with 1% methyl cellulose. At 72 hpi, the cells were observed using an epifluorescence microscope to visualize the expression of EGFP.
Total cellular RNA was isolated with TRIzol reagent according to standard protocols. One-step qRT-PCR was performed with HiScript® II One Step qRT-PCR SYBR® Green Kit (Vazyme, China) using an Applied Biosystems 7500 real-time thermocycler (Applied Biosystems, USA). The target gene and GAPDH transcript levels were determined using the ΔΔCT method. To determine the amount of purified JFH-1 virions, the pUC18-JFH1 plasmid was used as a standard sample to generate a standard curve ranging from 103–1011 copies/mL. JFH-1 RNA copies were quantified using the HiScript® II One Step qRT-PCR SYBR® Green Kit (Vazyme, China).
Western blot analysis
Cells were harvested in lysis buffer containing 50 mmol/L Tris-HCl (pH 8.0), 150 mmol/L NaCl, 1% Nonidet P-40, 0.1% SDS, 2 mmol/L EDTA and a protease inhibitor cocktail (Roche, Swiss). The proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore, USA). The membranes were sequentially blocked overnight at 4 °C with 5% nonfat dry milk solution in Tris-buffered saline (TBS). The membranes were then blotted with specific primary antibodies for 1 h and washed 3 times for 5 min with TBS containing 0.1% Tween-20 (v/v), followed by incubation with secondary antibodies conjugated to horseradish peroxidase for 45 min and washed 5 times for 5 min with TBS containing 0.1% Tween-20 (v/v) at room temperature. The proteins were visualized by chemiluminescence by using a Clarity Western ECL Substrate (Bio-rad, USA).
The cells were fixed with 4% paraformaldehyde for 15 min at room temperature, washed, and permeabilized with 0.05% Triton X-100 in PBS for 10 min. The cells were then washed and blocked with 2% BSA in PBS for 30 min, followed by 1 h incubation with primary antibodies targeting 5-HT2AR or CLDN-1. After three washes with PBS, the cells were incubated with secondary antibodies for 1 h. After washing with PBS, nuclei were stained with DAPI. Images were captured using a Nikon A1 Confocal Microscope.
Binding of virus to host cells
The virus binding assay was performed using a previously reported protocol, with modifications (Qing et al., 2016). Huh7.5.1 cells were seeded at 1 × 105 cells per well overnight. Then the cells were incubated with 0.5% DMSO or 10 μmol/L PBZ for 24 h. The following day, the culture medium was removed and the cells were washed with cold phosphate-buffered saline (PBS). HCV JFH-1 virus was subsequently diluted in 500 μL of DMEM complete medium (dilution fold = 1:2) (2 × 106 HCV RNA copies) and added to the cells. After 2 h of incubation at 4 °C, the unbound virus was removed by three wash steps with 500 μL of PBS, and the cells were lysed in with 500 μL TRIzol. Viral RNA was extracted and detected by qRT-PCR. Huh7.5.1 cells containing sh-NC or sh-5HT2AR were seeded at 1 × 105 cells per and virus binding was detected following the above method.
Animal welfare and experimental procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals (Ministry of Science and Technology of China), and were approved by the Animal Ethics Committee of Wuhan Institute of Virology. We used 10- to 12-week of age- and gender-matched mice for in vivo experiments. Human CD81 and occludin transgenic mice C/OTg mice (Chen et al., 2014) were divided in 3 batches and injected with HCV (JC-1 strain). A total of four to six mice were included in each group. Because C/OTg mice are immune-competent, we use a method as infection and treatment by considering the sustainability of the persistent infection mouse model in C/OTg mice. For infection in vivo, mice were tail-vein injected with 1 mL HCV at TCID50 = 5 × 107 in 60–120 s to avoid liver injury. For monotherapy, PBZ, VX-950 (Vertex Pharma, MA) or DMSO (Sigma, USA) were intraperitoneal injection administrated daily at one week post HCV infection. On day 0, we intravenously inoculated mice with JC-1. One week later, mice were treated daily with 0.2, 1, 2, or 4 mg PBZ per kg body weight via peritoneal injection for a total of 1–2 weeks. Negative control mice were treated via peritoneal injection with DMSO alone (100 µL per 20 g body weight) and positive control mice were treated with 20 mg per kg body weight VX-950 in DMSO (100 µL per 20 g body weight). Liver tissues and sera were collected at indicated time. The sera (300 μL for each mouse) were collected under anesthesia by extracting the eyeball blood. Mice were then sacrificed for liver tissues for HCV genome measurements. Total RNA was extracted from liver or serum using Trizol or Trizol LS reagent (Invitrogen, USA). qRT-PCR analyses with Artus HCV RG RT-PCR kit (Qiagen, Germany).
Flow cytometry was performed in LSR Fortessa (BD Biosciences, NJ) after cells (1 × 106) were incubated with CLDN1 antibody (10 μg/mL each) 1 h followed by the treatment with FITC-goat anti rabbit second-antibody (10 μg/mL each) for 30 min in PBS containing 2% FBS. Data were analyzed with FlowJo Software (Tree Star, USA) and represented three independent experiments.
Cell membranes and cytosolic fractions were freshly prepared from cells by using a Membrane Protein Extraction Kit (Thermofisher, USA). Briefly, cells were resuspended and centrifuged at 300 × g for 5 min. The cell pellet was washed with cell wash buffer for twice. After carefully removed the supernatant, 0.75 mL of permeabilization buffer was added to the cell pellet, vortex shortly, and then incubates 10 min at 4 °C with constant mixing. Centrifuge permeabilized cells for 15 min at 16,000×g. The supernatant containing cytosolic proteins was carefully removed and transferred to a new tube. 0.5 mL of solubilization buffer was added to the pellet, and resuspended by pipetting up and down and incubate tubes at 4 °C for 30 min. Centrifuge tubes at 16,000×g for 15 min at 4 °C. Transfer supernatant containing solubilized membrane and membrane-associated proteins to a new tube for further analysis.
Interaction of PBZ with other inhibitors of HCV proliferation
Huh 7.5.1 cells were seeded at 3 × 104 cells per well in 96-well microtiter plates. After an overnight culture at 37 °C, a different final concentration of PBZ (0, 0.25, 0.74, 2.22, 6.67, and 20 μmol/L) was added to each row of the 96-well plate. Simultaneously, a different final concentration of other HCV inhibitors was added to each column of the plate. Huh 7.5.1 cells were infected with the HCVcc virus after treatment with the two inhibitors and incubated for 48 h. Antiviral activities were determined by measuring the reduction of luciferase activity in the cells.
We thank Prof. Yin Z. for providing chemical probes, and Apath L.L.C. for gifting Huh7.5.1 cells and wild-type HCV JFH-1 virus.
5-HT2AR, serotonin 2A receptor; ARs, adrenergic receptors; CLDN1, claudin 1; DAAs, direct-acting antivirals; ECLs, extracellular loops; GPCRs, G protein-coupled receptors; HBV, hepatitis B virus; HCV, hepatitis C virus; HCVpp, HCV pseudotyped virus; HCVrep, HCV subgenomic replicon; HCVcc, cell culture-derived HCV; LDLR, low-density lipoprotein receptor; NPC1L1, Niemann-Pick C1-like 1; NTD, N-terminal fragment; OCLN, occluding; PBZ, phenoxybenzamine; PEG-IFN, pegylated interferon; PHHs, primary human hepatocytes; SR-BI, scavenger receptor class B type 1; SRFBP1, serum response factor binding protein 1; TfR-1, transferrin receptor 1; VLDLR, very-low-density lipoprotein receptor
COMPLIANCE WITH ETHICS GUIDELINES
Lin Cao, Jizheng Chen, Yaxin Wang, Yuting Yang, Jie Qing, Zihe Rao, Xinwen Chen, and Zhiyong Lou declare that they have no conflict of interest. This article does not contain any studies with human subjects performed by the any of the authors.
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