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Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles

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Abstract

Curcumin has various pharmacological activities such as anti-inflammatory, antioxidant, antimicrobial and antitumor activity. However, its use has been limited due to its poor solubility in water and minimal systemic bioavailability. Thus, we developed curcumin-delivering heparin-based nanoparticles and evaluated the inhibition effects of osteoclastogenesis by curcumin-delivering heparin nanoparticles (Cur-HD NPs). Cur-HD NPs were dispersed well in aqueous solution by forming self-assembled nanoparticles and showed sustained release of curcumin. In vitro studies, HD NPs facilitated intracellular delivery of curcumin into macrophages and osteoclasts, and thus, Cur-HD NPs effectively inhibited osteoclastogenesis in a dose-dependent manner by suppressing tartrate-resistant acid phosphatase (TRAP) activity and TRAP-positive multinucleated cells as well as by reducing the expression of osteoclast marker genes (i.e., TRAP and nuclear factor of activated T cells cytoplasmic 1 (NFATc1)). Furthermore, Cur-HD NPs markedly stimulated apoptosis of osteoclasts. Therefore, we hope that Cur-HD NPs will be useful nanodrugs for the treatment of bone-related diseases.

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Authors and Affiliations

  1. Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical Center, Guro Hospital, Seoul, 152-703, Korea

    Young-Pil Yun, Sung Eun Kim, Jae Yong Lee & Hae-Ryong Song

  2. Department of Orthopedic Surgery, Korea University Medical Center, Guro Hospital, Seoul, 152-703, Korea

    Hak-Jun Kim

  3. Department of Biotechnology, The Catholic University of Korea, Gyeonggi, 420-743, Korea

    Sung-Wook Choi

  4. Division of Bioimaging, Chuncheon Center, Korea Basic Science Institute, Gangwon, 200-701, Korea

    Kyeongsoon Park

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  1. Young-Pil Yun
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  2. Sung Eun Kim
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Correspondence to Kyeongsoon Park.

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These authors are equally contributed to this work.

The image from this article is used as the cover image of the Volume 22, Issue 6.

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Yun, YP., Kim, S.E., Lee, J.Y. et al. Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles. Macromol. Res. 22, 647–656 (2014). https://doi.org/10.1007/s13233-014-2082-1

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