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Evaluation of GENESIS-BCP™ scaffold composed of hydroxyapatite and β-tricalcium phosphate on bone formation

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Abstract

The purpose of this study was to evaluate the effects of a GENESIS-BCP™ scaffold composed of hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) on bone formation. The relative ratio of components forming GENESIS-BCPTM was investigated by confirming the molar ratio of Ca/P through energy dispersive spectrometer (EDS) analysis. The results being that the scaffold is comprised of 60% HAp and 40% β-TCP. Moreover, scanning electron microscopy (SEM) images showed a unique surface morphology similar to a soccer ball. X-ray diffraction (XRD) analysis showed that the porosity, crystallinity, and Ca/P ratio were 65.5%, 84.9%, and 1.61%, respectively. In vitro tests including cytotoxicity and cell proliferation showed a good biocompatibility of GENESIS-BCP™. In vivo tests, including mouse skull test, and hematoxylin and eosin stains of GENESIS-BCP™ implanted in rabbit and human subjects showed that the bone graft is effective to accelerate new bone formation. It was concluded that GENESIS-BCP™ can be used as an effective alloplast bone graft.

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Authors and Affiliations

  1. Department Maxillofacial Biology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

    Dae Hyeok Yang, Ha Na Park, Min Soo Bae, Jung Bok Lee, Dong Nyoung Heo & Il Keun Kwon

  2. Department Chemical & Biomolecular Engineering Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 121-742, Korea

    Won Jun Lee

  3. DIO Co., Busan, 612-020, Korea

    Young Min Park & Young Hwan Cho

  4. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

    Duck-Su Kim

Authors
  1. Dae Hyeok Yang
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  2. Ha Na Park
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  3. Min Soo Bae
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  4. Jung Bok Lee
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  5. Dong Nyoung Heo
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  6. Won Jun Lee
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  7. Young Min Park
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  8. Young Hwan Cho
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  9. Duck-Su Kim
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  10. Il Keun Kwon
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Correspondence to Il Keun Kwon.

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Yang, D.H., Park, H.N., Bae, M.S. et al. Evaluation of GENESIS-BCP™ scaffold composed of hydroxyapatite and β-tricalcium phosphate on bone formation. Macromol. Res. 20, 627–633 (2012). https://doi.org/10.1007/s13233-012-0090-6

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  • DOI: https://doi.org/10.1007/s13233-012-0090-6

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