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Multilocus analysis of the Exophiala jeanselmei clade containing black yeasts involved in opportunistic disease in humans

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Abstract

To confirm species delimitations in the ‘jeanselmei-clade’ in the Chaetothyriales, four independent markers were analysed, and phylogenetic trees were reconstructed using different algorithms. Reproductive isolation within the complex and reproductive modes in the species involved were determined, in order to explore specific borderlines using Genealogical Concordance Phylogenetic Species Recognition (GCPSR). Sequences of the Internal Transcribed Spacer (ITS), partial Translation Elongation Factor 1-α (TEF1), β-Tubulin (BT2), and Actin (ACT1) genes were analysed for a set of 81 strains. Phylogenetic reconstruction was performed using neighbour-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian analysis (BA) to evaluate the concordance of topologies obtained under different optimization criteria. Incongruence among lineages was detected by Partition Homogeneity Test (PHT). Most Exophiala species analysed in this study seemed to be preponderantly clonal. A group of species around Exophiala spinifera showed a tendency toward human pathogenicity, whereas species around E. bergeri were often associated with environments rich in hydrocarbons. Though the tree topologies were not completely identical when different algorithms were used, the four gene lineages were nearly congruent and the trees of the combined multilocus data set did not provide more phylogenetic information than those of separate data sets. ITS data are sufficient for identification of species in the ‘jeanselmei-clade’.

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Acknowledgments

K.F. Luijsterburg is thanked for technical assistance. D.A. Sutton (Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center, San Antonio, Texas, U.S.A.) is acknowledged for providing a large number of clinical isolates, for which E.H. Thompson and J. Ruiz performed morphological identification.

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Authors and Affiliations

  1. Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD, Utrecht, the Netherlands

    Jingsi Zeng, Peiying Feng, A. H. G. Gerrits van den Ende, M. J. Harrak & G. S. de Hoog

  2. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands

    Jingsi Zeng, M. J. Harrak & G. S. de Hoog

  3. Department of Dermatology and Venereology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, China

    Jingsi Zeng

  4. Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

    Peiying Feng

  5. Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China

    Liyan Xi & G. S. de Hoog

  6. Peking University Health Science Center, Research Center for Medical Mycology, Beijing, China

    G. S. de Hoog

  7. Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China

    G. S. de Hoog

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  1. Jingsi Zeng
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  2. Peiying Feng
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  3. A. H. G. Gerrits van den Ende
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  4. Liyan Xi
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  5. M. J. Harrak
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  6. G. S. de Hoog
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Correspondence to G. S. de Hoog.

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Zeng, J., Feng, P., van den Ende, A.H.G.G. et al. Multilocus analysis of the Exophiala jeanselmei clade containing black yeasts involved in opportunistic disease in humans. Fungal Diversity 65, 3–16 (2014). https://doi.org/10.1007/s13225-013-0226-9

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  • DOI: https://doi.org/10.1007/s13225-013-0226-9

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