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Estradiol Level on Day 2 and Day of Trigger: A Potential Predictor of the IVF-ET Success

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Abstract

Objective

To evaluate role of serum estradiol levels in predicting likelihood of pregnancy in women undergoing GnRH-a protocol in IVF-ET cycles.

Design

A 3-year retrospective analysis of estradiol levels on down-regulated day 2, day 6, and day of hCG trigger and subsequent clinical pregnancy rates.

Setting

A university hospital tertiary referral centre.

Population or Sample

Women undergoing IVF treatment.

Methods

Hormonal assessment on the down-regulated day 2, day 6, and day of hCG trigger.

Main Outcome Measure(s)

Comparison of hormonal profile, antral follicular count on day 2, endometrial thickness on day of trigger, and number of oocytes retrieved between pregnant and the non-pregnant group. The prediction of IVF success was based on the quantitative levels of estradiol on a specific day in down-regulated cycle.

Result(s)

The overall pregnancy rate was 32.25 % (50/160). Estradiol level on down-regulated day 2 was 31.9 ± 12.6 and on the day of trigger was 1,996.46 ± 1,252.36 in pregnant women, which was significantly higher as compared to estradiol levels in non-pregnant women (27.6 ± 12.3 and 1,525.1 ± 1,116.42, respectively). It was found to be a significant prognostic marker for successful IVF treatment. Estradiol levels on down-regulated day 6 were found to be non-significant between the two groups.

Conclusion(s)

Estradiol level on down-regulated day 2 of menstrual cycle and on the day of trigger was found to have a significant impact on the success of IVF-ET.

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References

  1. Evers JL. Female subfertility. Lancet. 2002;360:151–9.

    Article  PubMed  Google Scholar 

  2. 2009 Assisted Reproductive Technology, Success Rates, National Summary and Fertility Clinic Reports. National Centers for Chronic Disease Prevention and Health Promotion, 2011.

  3. Scott RT, Toner JP, Muasher SJ, et al. Follicle-stimulating hormone levels on cycle day 3 are predictive of in vitro fertilization outcome. Fertil Steril. 1989;4:651–4.

    Google Scholar 

  4. Licciardi FL, Liu HC, Rosemwaks Z et al. Day 3 estradiol serum concentrations as prognosticators of ovarian stimulation response and pregnancy outcome in patients undergoing in vitro fertilization. Fertil Steril. 1995;5:991–4.

    Google Scholar 

  5. Yanushpolsky EH, Hurwitz S, Tikh E. Predictive usefulness of cycle day 10 follicle-stimulating hormone level in a clomiphene citrate challenge test for in vitro fertilization in women younger than 40 years of age. Fertil Steril. 2003;1:111–5.

    Article  Google Scholar 

  6. Hofmann GE, Danforth DR, Seifer DB. Inhibin-B: The physiologic basis of the clomiphene citrate challenge test for ovarian reserve screening. Fertil Steril. 1998;3:474–7.

    Article  Google Scholar 

  7. Tomas C, Nuojua-Huttunen S, Martinaken H. Pretreatment transvaginal ultrasound examination predicts ovarian responsiveness to gonadotrophins in in vitro fertilization. Hum Reprod. 1997;2:220–3.

    Article  Google Scholar 

  8. Hendriks DJ, Mol BW, Bancsi LF et al. Antral follicle count in the prediction of poor ovarian response and pregnancy after in vitro fertilization: a meta-analysis and comparison with basal follicle-stimulating hormone level. Fertil Steril. 2005;2:291–301.

    Article  Google Scholar 

  9. Ficicioglu C, Kutlu T, Baglam E, et al. Early follicular antimullerian hormone as an indicator of ovarian reserve. Fertil Steril. 2006;3:592–6.

    Article  Google Scholar 

  10. Hull MG, Fleming CF, Hughes AO, et al. The age related decline in female fecundity: a quantitative controlled study of implanting capacity and survical of individual embryos after in vitro fertilization. Fertil Steril. 1996;4:783–90.

    Google Scholar 

  11. Scott RT, Hofmann GE, Oehninger S, et al. Intercycle variability of day 3 follicle-stimulating hormone levels and its effect on stimulation quality in in vitro fertilization. Fertil Steril. 1990;2:297–302.

    Google Scholar 

  12. Ocal P, Aydin S, Cepni J, et al. Follicular fluid concentrations of vascular endothelial growthfactor, inhibin A, inhibin B in IVF cycles: are they markers for ovarian response and pregnancy outcome. Eur J Obstet Gynecol Reprod Biol. 2004;2:194–9.

    Article  Google Scholar 

  13. Khalaf Y, Taylor A, Braude P. Low serum E2 concentrations after five days of controlled ovarian hyperstimulation for in vitro fertilization are associated with poor outcome. Fertil Steril. 2000;74:63–6.

    Article  CAS  PubMed  Google Scholar 

  14. Devroey P, Bourrgain C, Macklon NS, et al. Reproductive biology and IVF: ovarian stimulation and endometrial receptivity. Trends Endocrinol Metab. 2004;15:84–90.

    Article  CAS  PubMed  Google Scholar 

  15. Anifandis G, Koutselini E, Louridas K, et al. Estradiol and leptin as conditional prognostic IVF markers. Reproduction. 2005;129:531–4.

    Article  CAS  PubMed  Google Scholar 

  16. Papageorgiou T, Guibert J, Goffinet F et al. Percentile curves of serum estradiol levels during controlled ovarian stimulation in 905 cycles stimulated with recombinant FSH show that high estradiol is not detrimental to IVF outcome. Hum Reprod. 2002;17:2846–50.

    Article  CAS  PubMed  Google Scholar 

  17. Speroff L, Glass RH, Kase AG. Regulation of menstrual cycle. Clinical gynecologic endocrinology and infertility. Maryland: Williams & Wilkins; 1994. p. 141–83.

    Google Scholar 

  18. Tesarik J, Mendoza C. Nongenomic. Effects of 17 beta-estradiol on maturing human oocytes: relationship to oocyte developmental potential. J Clin Endocrinol Metab. 1995;80:1438–43.

    CAS  PubMed  Google Scholar 

  19. Weghofer A, Margreiter M, Fauster Y et al. Age-specific FSH levels as a tool for appropriate patient counseling in assisted reproduction. Hum Reprod. 2005;20(9):2448–52.

    Article  PubMed  Google Scholar 

  20. Coroleu B, Barri PN, Carreras O et al. The influence of the depth of embryo replacement into the uterine cavity on implantation rates after IVF: a controlled, ultrasound-guided study. Hum Reprod. 2002;17(2):341–6.

    Article  PubMed  Google Scholar 

  21. Tomas C, Tikkinen K, Tuomivaara L et al. The degree of difficulty of embryo transfer is an independent factor for predicting pregnancy. Hum Reprod. 2002;17(10):2632–5.

    Article  PubMed  Google Scholar 

  22. Edgar DH. Estrogen and human implantation. Hum Reprod. 1995;10:2–4.

    Article  CAS  PubMed  Google Scholar 

  23. Makker A, Singh MM. Endometrial receptivity: clinical assessment in relation to fertility, infertility, and antifertility. Med Res Rev. 2006;26(6):699–746.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors deeply acknowledge the patients for giving consent for the study. Technical assistance from staff of IVF and Reproductive Biology Centre and Department of Biochemistry and Biotechnology is highly acknowledged.

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, IVF & Reproductive Biology Centre, Maulana Azad Medical College, Bahadur Shah Zafar Marg, New Delhi, 110002, India

    Sudha Prasad (Director Professor and IVF Coordinator), Yogesh Kumar & Megha Singhal

  2. Institute of Cytology & Preventive Oncology (ICMR), Sec 39, Noida, Uttar Pradesh, India

    Shashi Sharma

Authors
  1. Sudha Prasad
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  2. Yogesh Kumar
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  3. Megha Singhal
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  4. Shashi Sharma
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Correspondence to Sudha Prasad.

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Prasad, S., Kumar, Y., Singhal, M. et al. Estradiol Level on Day 2 and Day of Trigger: A Potential Predictor of the IVF-ET Success. J Obstet Gynecol India 64, 202–207 (2014). https://doi.org/10.1007/s13224-014-0515-6

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  • DOI: https://doi.org/10.1007/s13224-014-0515-6

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