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Simultaneous saccharification and fermentation of pretreated sugarcane bagasse to ethanol using a new thermotolerant yeast

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Abstract

Enzymatic hydrolysis of a cellulosic substrate is the most critical step for the production of bioethanol. In our study, the hydrolysis of steam-exploded sugarcane bagasse (SESB) under optimized conditions (8 % substrate consistency, 22.5 U filter paper cellulase, 0.55 % Tween 80) released a maximum of 461 mg per gram dry substrate sugars. We isolated a thermotolerant yeast strain, Blastobotrys adeninivorans RCKP 2012, from sugarcane bagasse collected from the Cooperative Sugar Mill, Sonepat, Haryana that was found to be capable of fermenting the enzymatic hydrolysate of SESB at 50 °C. When grown under simultaneous saccharification and fermentation conditions, this yeast produced 14.05 g L−1 ethanol, which corresponds to a theoretical ethanol yield of 46.87 %.

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Acknowledgments

The authors acknowledge the support from the University of Delhi for providing the facilities and finances to carry out the work. The authors also acknowledge the technical support of Mr. Subhojit Chakraborty. Rishi Gupta also acknowledges the Council of Scientific and Industrial Research for providing a Senior Research Fellowship.

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Authors and Affiliations

  1. Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Parul Singh Antil, Rishi Gupta & Ramesh Chander Kuhad

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  1. Parul Singh Antil
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  2. Rishi Gupta
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  3. Ramesh Chander Kuhad
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Correspondence to Ramesh Chander Kuhad.

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Antil, P.S., Gupta, R. & Kuhad, R.C. Simultaneous saccharification and fermentation of pretreated sugarcane bagasse to ethanol using a new thermotolerant yeast. Ann Microbiol 65, 423–429 (2015). https://doi.org/10.1007/s13213-014-0875-2

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  • DOI: https://doi.org/10.1007/s13213-014-0875-2

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