Abstract
This study aimed to determine the pattern of antibiotic and heavy metal resistance in Gram-negative bacteria isolated from five different sites in the Seyhan Dam Lake and Seyhan River in Adana, Turkey. The susceptibility of 268 isolates to 16 different antibiotics and five heavy metals was investigated by agar diffusion and dilution methods, respectively. The most common species isolated from the samples were Aeromonas hydrophila (17.5 %), Aeromonas caviae (8.9 %) and Citrobacter freundii (8.9 %). There was a high incidence of resistance to ampicillin (80.2 %), streptomycin (71.6 %) and cefazolin (60.4 %). Multiple antibiotic resistance indices ranged from 0.2 to 0.81, suggesting exposure to antibiotic contamination. The isolates showed tolerance to different concentrations of heavy metals. These results indicate that antibiotic and heavy metal resistance among the Seyhan Dam Lake and Seyhan River bacteria may pose a risk to the fish population and public health. At the same time, the finding in the aquatic environments of different combinations of resistance genes suggests their involvement in the spread of multidrug-resistant strains.
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Akinbowale OL, Peng H, Grant P, Barton MD (2007) Antibiotic and heavy metal resistance in motile aeromonads and pseudomonads from rainbow trout (Oncorhynchus mykiss) farms in Australia. Int J Antimicrob Agents 30:177–182
Alonso A, Sanchez P, Martinez JL (2001) Environmental selection of antibiotic resistance genes. Environ Microbiol 3:1–9
Altug G, Balkis N (2009) Levels of some toxic elements and frequency of bacterial heavy metal resistance in sediment and sea water. Environ Monit Assess 149:61–69
APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington, DC
Borrell N, Figueras MJ, Guarro J (1998) Phenotypic identification of aeromonas genospecies from clinical and environmental sources. Can J Microbiol 44:103–108
Cardonha AMS, Viera RHSF, Rodrigues DP, Macrae A, Peirano G, Teophilo GND (2004) Fecal pollution in water from storm sewers and adjacent seashores in Natal, Rio Grande do Norte, Brazil. Int Microbiol 7:213–218
Cumberbatch N, Gurwith MJ, Langston Sack RB, Brunton JL (1979) Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrhoeal disease. Infect Immun 23:829–837
De Sousa MJ, Nair S, Bharathi PAL, Chandramohan D (2006) Metal and antibiotic-resistance in psychrotrophic bacteria from Antarctic Marine Waters. Ecotoxicology 15:379–384
Fernàndez-Delgado M, Suàrez P (2009) Multiple antibiotic resistance of enteric bacteria isolated from recreational coastal waters and oysters of the Caribbean Sea. Ann Microbiol 59:409–414
Gul-Seker M, Mater Y (2009) Assessment of metal and antibiotic-resistance in marine bacteria isolated from Izmit Bay and Bosphorus entrance of Marmara and Black Sea, Turkey. Fresen Environ Bull 18:2192–2202
Kaper JB, Nataro JP, Mobley HL (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2:123–140
Kim KR, Owens G, Kwon SI (2011) Occurence and environmental fate of veterinary antibiotics in the terrestrial environment. Water Air Soil Pollut 214:163–174
Krumperman PH (1983) Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46:165–170
Lee SW, Najiah M, Wendy W, Zahrol A, Nadirah M (2009) Multiple antibiotic resistance and heavy metal resistance profile of bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) hatchery. Agric Sci China 8:740–745
Lemos ML, Toranzo AE, Barja JL (1985) Modified medium for the oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 49:1541–1543
Lopez-Maury L, Garcia-Dominguez M, Florencio FJ, Reyes JC (2002) A two component signal transduction system involved in nickel sensing in the Cyanobacterium synechocyystis sp. PCC 6803. Mol Microbiol 43:247–256
Matlock MM, Henke KR, Atwood DA (2002) Effectiveness of commercial reagents for heavy metal removal from water with new insights for future chelate designs. J Hazard Mater 92:129–142
Matyar F (2007) Distribution and antimicrobial multiresistance in Gram-negative bacteria isolated from Turkish sea bass (Dicentrarchus labrax L., 1781) farm. Ann Microbiol 57:35–38
Matyar F (2012) Antibiotic and heavy metal resistance in bacteria isolated from Mediterranean sea coast. B Environ Contam Tox 89:551–556
Matyar F, Kaya A, Dincer S (2008) Antibacterial agents and heavy metal resistance in Gram-negative bacteria isolated from seawater, shrimp and sediment in Iskenderun Bay, Turkey. Sci Total Environ 407:279–285
Matyar F, Akkan T, Ucak Y, Eraslan B (2010) Aeromonas and Pseudomonas:antibiotic and heavy metal resistance species from Iskenderun Bay, Turkey (northeast Mediterranean Sea). Environ Monit Assess 167:309–320
Miranda CD, Castillo G (1998) Resistance to antibiotic and heavy metals of motile aeromonads from Chilean freshwater. Sci Total Environ 224:167–176
NCCLS (1997) Performance standards for antimicrobial disk susceptibility tests, 6th edn. National committee for clinical laboratory standards approved standards M2-A6. NCCLS, Wayne
NCCLS (2002) Performance standards for antimicrobial disk susceptibility tests, 7th edn. National committee for clinical laboratory standards document M2–A7, vol 20. NCCLS, Villanova
Obi CL, Bessong PO, Momba MNB, Potgieter N, Samie A, Igumbor EO (2004) Profiles of antibiotic susceptibilities of bacterial isolates and physic-chemical quality of water supply in rural Venda communities, South Africa. Water SA 30:515–519
Pathak SP, Gopal K (2007) Bacterial contamination and antibiotic resistance in fecal coliforms from glacial water runoff. B Environ Contam Tox 79:163–167
Pepperell C, Kus JV, Gardam MA, Humar A, Burrows LL (2002) Low virulence Citrobacter species encode resistance to multiple antimicrobials. Antimicrob Agents Chemother 46:3555–3560
Rani DBR, Mahedevan A (1993) Patterns of heavy metal resistance in marine Pseudomonas MRI. Indian J Exp Biol 31:682–686
Silver S (1998) Genes for all metals—a bacterial view of the periodic table. J Ind Microbiol Biot 20:1–12
Somasundaram L, Coats JR, Racke DK, Stahr HM (1990) Application of the microtox system to assess the toxicity of pesticides and their hydrolysis metabolites. B Environ Contam Tox 44:254–259
Summers AO (2002) Generally overlooked fundamentals of bacterial genetics and ecology. Clin Infect Dis 34:85–92
Vaseeharan B, Ramasamy P, Murugan T, Chen JC (2005) In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int J Antimicrobl Ag 26:285–291
Wilkes G, Edge T, Gannon V, Jokinen C, Lyautey E, Mederios D, Neumann N, Reucker N, Topp E, Lapen DR (2009) Seasonal relationships among indicator bacteria, pathogenic bacteria, Cryptosporidium oocysts, Giardia cysts, and hydrological indices for surface waters within an agricultural landscape. Water Res 43:2209–2223
Acknowledgment
We would like to thank BAPCOM (Cukurova University) for financially supporting this work (Project number: EF20010BAP12).
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Matyar, F., Gülnaz, O., Guzeldag, G. et al. Antibiotic and heavy metal resistance in Gram-negative bacteria isolated from the Seyhan Dam Lake and Seyhan River in Turkey. Ann Microbiol 64, 1033–1040 (2014). https://doi.org/10.1007/s13213-013-0740-8
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DOI: https://doi.org/10.1007/s13213-013-0740-8