Skip to main content
SpringerLink
Log in

Phylogenetic relatedness of Xanthomonas axonopodis pv. punicae, the causal agent of bacterial blight of pomegranate based on two loci, 16S rRNA and gyrB

  • Short Communication
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

Bacterial blight caused by Xanthomonas axonopodis pv. punicae (Xap) is a major disease in pomegranate (Punica granatum) cultivation in India. The Xap strains from three distinct geographical origins, Delhi, Maharashtra and Andhra Pradesh were studied for their genetic variability and phylogenetic relationship with other Xanthomonads targeting two important loci 16S rRNA and gyrB. All Xap strains showed 100 % sequence conservation in both the loci, suggesting that geographical origin does not necessarily reflect variation to genetic make-up of the Xap. Phylogeny derived from 16S rRNA gene revealed that two Xanthomonas species, Xanthomonas citri subsp. malvacearum DSM 3849 T and X. axonopodis pv. manihotis NCPPB1834 formed a single cluster along with Xap. Further, analysis in the gyrB locus indicated that X. citri subsp. malvacearum shared 99.4 % identity while pathovars X. axonopodis pv. manihotis shared only 95 % identity with the Xap strains. Thus, we established that gyrB was the preferred locus over 16S rRNA gene to discriminate the Xap strains from closely related Xanthomonas species type strains. Nevertheless, our study demonstrated for the first time that pomegranate bacterial blight pathogen is phylogenetically very close to Xanthomonas citri subsp. malvacearum infecting cotton.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Adachi N, Oku T (2000) PCR-mediated detection of Xanthomonas oryzae pv. oryzae by amplification of the 16S-23S rDNA spacer region sequence. J Gen Plant Pathol 66:303–309

    Article  CAS  Google Scholar 

  • Adhikari TB, Vera Cruz CM, Zhang Q, Nelson RJ, Skinner DZ, Mew TW, Leach JE (1995) Genetic diversity of Xanthomonas oiyzae pv. oryzae in Asia. Appl Environ Microbiol 61:966–971

    PubMed  CAS  Google Scholar 

  • Akhtar MA, Bhatti MHR (1992) Occurrence of bacterial leaf spot of pomegranate in Pakistan. Pak J Agric Res 13:95–97

    Google Scholar 

  • Ash C, Farrow JAE, Dorsch M, Stackebrandt E, Collins MD (1991) Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species based on the basis of reverse transcriptase sequencing of 16S rRNA. Int J Syst Bacteriol 41:343–346

    Article  PubMed  CAS  Google Scholar 

  • Chand R, Kishun R (1991) Studies on bacterial blight of pomegranate. Indian Phytopathol 44:370–372

    Google Scholar 

  • Hauben L, Vauterin L, Swings J, Moore ERB (1997) Comparison of 16S ribosomal DNA sequences of all Xanthomonas species. Int J Syst Bacteriol 47:328–335

    Article  PubMed  CAS  Google Scholar 

  • Hingorani MK, Mehta PP (1952) Bacterial leaf spot of pomegranate. Indian Phytopathol 5:55–56

    Google Scholar 

  • Kumar R, Shamarao Jahagirdar MR, Yenjerappa ST, Patil HB (2009) Epidemiology and Management of bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae. Acta Horticult 818:291–296

    Google Scholar 

  • Mondal KK, Sharma J (2009) Bacterial blight: An emerging threat to pomegranate export. Indian Farming 59:22–23

    Google Scholar 

  • Mondal KK, Mani C (2009) ERIC-PCR generated genomic fingerprints and their correlation with pathogenic variability of Xanthomonas campestris pv. punicae, the incitant of bacterial blight of pomegranate. Curr Micribiol 59:616–620

    Article  CAS  Google Scholar 

  • Mondal, KK and Mani, C (2012) Investigation of the antibacterial properties of nanocopper against Xanthomonas axonopodis pv. punicae, the incitant of pomegranate bacterial blight. Ann Microbiol 62:889–893

  • Nesme X, Vaneechoutte M, Orso S, Hoste B, Swings J (1995) Diversityand genetic relatedness within genera Xanthomonas and Stenotrophomonus using restriction endonuclease site differences of PCR-amplified 16s rRNA gene. Syst Appl Microbiol 18:127–135

    Article  CAS  Google Scholar 

  • Parkinson N, Cowie C, Heeney J, Stead D (2009) Phylogenetic structure of Xanthomonas determined by comparison of gyrB sequences. Int J Syst Evol Microbiol 59:264–274

    Article  PubMed  CAS  Google Scholar 

  • Petersen Y, Mansvelt EL, Venter E, Langenhoven WE (2010) Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomegranate in South Africa. J Australas Plant Pathol 39:544–546

    Article  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

Download references

Acknowledgments

The authors are thankful to Dr. V. G. Malathi, Division of Plant Pathology, IARI, New Delhi for critically reviewing the manuscript and for providing valuable inputs. The authors also acknowledge the Dr. R.K. Jain for his constant support and valuable suggestions.

Author information

Authors and Affiliations

  1. Indian Agricultural Research Institute, New Delhi, Delhi, India

    Kalyan K. Mondal, Geeta Verma & Chander Mani

Authors
  1. Kalyan K. Mondal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geeta Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chander Mani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kalyan K. Mondal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mondal, K.K., Verma, G. & Mani, C. Phylogenetic relatedness of Xanthomonas axonopodis pv. punicae, the causal agent of bacterial blight of pomegranate based on two loci, 16S rRNA and gyrB . Ann Microbiol 63, 801–804 (2013). https://doi.org/10.1007/s13213-012-0498-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-012-0498-4

Keywords

Search

Navigation