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Evaluation of immunoreactivity of in vitro and in vivo models against bacterial synthesized cellulose to be used as a prosthetic biomaterial

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Abstract

Prosthetic biomaterials are required to be non-toxic, non-thrombogenic, and non-immunogenic. Bacterial cellulose (BC) synthesized by Gluconacetobacter xylinus has recently been studied as a biocompatible material due to its unique features such as high purity, crystallinity, biodegradability, and tensile strength as compared to plant cellulose. Although BC has high potential to be used as biomaterial, its toxicity and immunoreactivity have not been properly studied yet. In this report, we investigated the immunoreactivity of BC in vitro in human umbilical vein endothelial cells (HUVECs) and in vivo using BALB/c mice. We report that BC does not induce apoptosis and necrosis in HUVECs and does not stimulate immune response in both HUVECs and BALB/c mice models. These results suggest that BC may be widely used as a biocompatible biomaterial for tissue engineering and biosensors.

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References

  1. Petersen, N. & Gatenholm, P. Bacterial cellulose-based materials and medical devices: current state and perspectives. Appl. Microbiol. Biotechnol. 91, 1277–1286 (2011).

    Article  CAS  Google Scholar 

  2. Klemm, D. et al. Nanocelluloses: A New Family of Nature-Based Materials. Angewandte Chemie-International Edition 50, 5438–5466 (2011).

    Article  CAS  Google Scholar 

  3. Kim, S.Y. et al. Production of bacterial cellulose by Gluconacetobacter sp. RKY5 isolated from persimmon vinegar. Appl. Biochem. Biotechnol. 131, 705–715 (2006).

    Article  Google Scholar 

  4. Falcao, S.C., Coelho, A.R. & Evencio Neto, J. Biomechanical evaluation of microbial cellulose (Zoogloea sp.) and expanded polytetrafluoroethylene membranes as implants in repair of produced abdominal wall defects in rats. Acta Cir. Bras. 23, 184–191 (2008).

    Article  Google Scholar 

  5. Brown, R.M., Jr., Willison, J.H. & Richardson, C.L. Cellulose biosynthesis in Acetobacter xylinum: visualization of the site of synthesis and direct measurement of the in vivo process. Proc. Natl. Acad. Sci. USA 73, 4565–4569 (1976).

    Article  CAS  Google Scholar 

  6. Ross, P., Mayer, R. & Benziman, M. Cellulose biosynthesis and function in bacteria. Microbiol. Rev. 55, 35–58 (1991).

    CAS  Google Scholar 

  7. Hutchens, S.A. et al. Biomimetic synthesis of calciumdeficient hydroxyapatite in a natural hydrogel. Biomaterials 27, 4661–4670 (2006).

    Article  CAS  Google Scholar 

  8. Helenius, G. et al. In vivo biocompatibility of bacterial cellulose. J. Biomed. Mater. Res. A. 76, 431–438 (2006).

    Google Scholar 

  9. Muller, D. et al. Structure and properties of polypyrrole/bacterial cellulose nanocomposites. Carbohydrate Polymers 94, 655–662 (2013).

    Article  CAS  Google Scholar 

  10. Teeri, T.T., Brumer, H., 3rd, Daniel, G. & Gatenholm, P. Biomimetic engineering of cellulose-based materials. Trends Biotechnol. 25, 299–306 (2007).

    Article  CAS  Google Scholar 

  11. Czaja, W., Krystynowicz, A., Bielecki, S. & Brown, R.M., Jr. Microbial cellulose — the natural power to heal wounds. Biomaterials 27, 145–151 (2006).

    Article  CAS  Google Scholar 

  12. Liang, Y. et al. A novel bacterial cellulose-based carbon paste electrode and its polyoxometalate-modified properties. Electrochemistry Communications 11, 1018–1021 (2009).

    Article  CAS  Google Scholar 

  13. Jeong, S.I. et al. Effect of alpha,beta-unsaturated aldehydes on endothelial cell growth in bacterial cellulose for vascular tissue engineering. Mol. Cell. Toxicol. 8, 119–126 (2012).

    Article  CAS  Google Scholar 

  14. Schumann, D.A. et al. Artificial vascular implants from bacterial cellulose: preliminary results of small arterial substitutes. Cellulose 16, 877–885 (2009).

    Article  CAS  Google Scholar 

  15. Klemm, D., Schumann, D., Udhardt, U. & Marsch, S. Bacterial synthesized cellulose — artificial blood vessels for microsurgery. Progress in Polymer Science 26, 1561–1603 (2001).

    Article  CAS  Google Scholar 

  16. Schumann, D.A. et al. New results about BASYC (R) (bacterial synthesized cellulose), the promising artificial blood vessel for microsurgery and further application of bacterial cellulose in medicine. Abstracts of Papers of the American Chemical Society 229, U300–U300 (2005).

    Google Scholar 

  17. Backdahl, H. et al. Mechanical properties of bacterial cellulose and interactions with smooth muscle cells. Biomaterials 27, 2141–2149 (2006).

    Article  Google Scholar 

  18. Lau, R.K.L. et al. Mechanical characterization of cellulosic thecal plates in dinoflagellates by nanoindentation. Journal of Nanoscience and Nanotechnology 7, 452–457 (2007).

    CAS  Google Scholar 

  19. Habibovic, P. et al. 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials 26, 3565–3575 (2005).

    Article  CAS  Google Scholar 

  20. Fink, H. et al. An in vitro study of blood compatibility of vascular grafts made of bacterial cellulose in comparison with conventionally-used graft materials. Journal of Biomedical Materials Research Part A 97A, 52–58 (2011).

    Article  CAS  Google Scholar 

  21. Wang, B. & Dong, S. Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film. Talanta 51, 565–572 (2000).

    Article  CAS  Google Scholar 

  22. Salomao, R. et al. Bacterial Sensing, Cell Signaling, and Modulation of the Immune Response during Sepsis. Shock 38, 227–242 (2012).

    Article  CAS  Google Scholar 

  23. Zhao, Q.T., Guo, Q.M., Wang, P. & Wang, Q. Salvianic acid A inhibits lipopolysaccharide-induced apoptosis through regulating glutathione peroxidase activity and malondialdehyde level in vascular endothelial cells. Chinese Journal of Natural Medicines 10, 53–57 (2012).

    Article  CAS  Google Scholar 

  24. Galanos, C. et al. Biological activities of lipopolysaccharides and lipid A from Rhodospirillaceae. Infect. Immun. 16, 407–412 (1977).

    CAS  Google Scholar 

  25. Mackensen, A., Galanos, C. & Engelhardt, R. Modulating Activity of Interferon-Gamma on Endotoxin-Induced Cytokine Production in Cancer-Patients. Blood 78, 3254–3258 (1991).

    CAS  Google Scholar 

  26. Dimmeler, S. & Zeiher, A.M. Endothelial cell apoptosis in angiogenesis and vessel regression. Circ. Res. 87, 434–439 (2000).

    Article  CAS  Google Scholar 

  27. Munoz, C. et al. Dysregulation of in vitro cytokine production by monocytes during sepsis. J. Clin. Invest. 88, 1747–1754 (1991).

    Article  CAS  Google Scholar 

  28. De Beaux, A.C. et al. Interleukin-4 and interleukin-10 increase endotoxin-stimulated human umbilical vein endothelial cell interleukin-8 release. J. Interferon. Cytokine Res. 15, 441–445 (1995).

    Article  Google Scholar 

  29. Le, J., Lin, J.X., Henriksen-DeStefano, D. & Vilcek, J. Bacterial lipopolysaccharide-induced interferongamma production: roles of interleukin 1 and interleukin 2. J. Immunol. 136, 4525–4530 (1986).

    CAS  Google Scholar 

  30. Eliopoulos, A.G. et al. Induction of COX-2 by LPS in macrophages is regulated by Tpl2-dependent CREB activation signals. EMBO J. 21, 4831–4840 (2002).

    Article  CAS  Google Scholar 

  31. Park, Y.S. et al. Acrolein induces cyclooxygenase-2 and prostaglandin production in human umbilical vein endothelial cells: roles of p38 MAP kinase. Arterioscler Thromb Vasc Biol. 27, 1319–1325 (2007).

    Article  CAS  Google Scholar 

  32. Caughey, G.E. et al. Roles of cyclooxygenase (COX)-1 and COX-2 in prostanoid production by human endothelial cells: selective up-regulation of prostacyclin synthesis by COX-2. J. Immunol. 167, 2831–2838 (2001).

    CAS  Google Scholar 

  33. Tough, D.F., Sun, S. & Sprent, J. T cell stimulation in vivo by lipopolysaccharide (LPS). J. Exp. Med. 185, 2089–2094 (1997).

    Article  CAS  Google Scholar 

  34. Yang, H.Y., Dundon, P.L., Nahill, S.R. & Welsh, R.M. Virus-induced polyclonal cytotoxic T lymphocyte stimulation. J. Immunol. 142, 1710–1718 (1989).

    CAS  Google Scholar 

  35. Wang, L. et al. High dose lipopolysaccharide triggers polarization of mouse thymic Th17 cells in vitro in the presence of mature dendritic cells. Cellular Immunology 274, 98–108 (2012).

    Article  CAS  Google Scholar 

  36. O’Sullivan, S.T. et al. Major injury leads to predominance of the T helper-2 lymphocyte phenotype and diminished interleukin-12 production associated with decreased resistance to infection. Ann. Surg. 222, 482–490; discussion 490–482 (1995).

    Google Scholar 

  37. Jeong, S.I. et al. Toxicologic evaluation of bacterial synthesized cellulose in endothelial cells and animals. Mol. Cell. Toxicol. 6, 373–380 (2010).

    Article  Google Scholar 

  38. Lee, S.E. et al. Upregulation of heme oxygenase-1 as an adaptive mechanism for protection against crotonaldehyde in human umbilical vein endothelial cells. Toxicology Letters 201, 240–248 (2011).

    Article  CAS  Google Scholar 

  39. Jeong, S.I. et al. Genome-wide analysis of gene expression by crotonaldehyde in human umbilical vein endothelial cells. Mol. Cell. Toxicol. 7, 127–134 (2011).

    Article  CAS  Google Scholar 

  40. Yang, H. et al. Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells. Journal of Ginseng Research 35, 352–359 (2011).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea

    Gun-Dong Kim, Hana Yang, Hye Rim Park, Cheung-Seog Park, Yong Seek Park & Seung Eun Lee

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  1. Gun-Dong Kim
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  2. Hana Yang
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  3. Hye Rim Park
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  4. Cheung-Seog Park
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  5. Yong Seek Park
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  6. Seung Eun Lee
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Correspondence to Yong Seek Park or Seung Eun Lee.

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Kim, GD., Yang, H., Park, H.R. et al. Evaluation of immunoreactivity of in vitro and in vivo models against bacterial synthesized cellulose to be used as a prosthetic biomaterial. BioChip J 7, 201–209 (2013). https://doi.org/10.1007/s13206-013-7302-9

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  • DOI: https://doi.org/10.1007/s13206-013-7302-9

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