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Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry

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Abstract

A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL−1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.

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Authors and Affiliations

  1. Food Safety Center, R&D Institute, Namyang Dairy Co., Ltd., Gongju, 314-914, Korea

    Jang-Hyuk Ahn

  2. Department of Biological Sciences, KAIST, Daejeon, 305-701, Korea

    Young-Su Jeong & Hak-Sung Kim

  3. Center for Nano-Bio Convergence, Korea Research Institute of Standards and Science (KRISS), Daejeon, 305-600, Korea

    Tae Geol Lee

  4. Department of Life Science, Hanyang University, Seoul, 133-791, Korea

    Young-Pil Kim

Authors
  1. Jang-Hyuk Ahn
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  2. Young-Su Jeong
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  3. Tae Geol Lee
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  4. Young-Pil Kim
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  5. Hak-Sung Kim
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Correspondence to Young-Pil Kim or Hak-Sung Kim.

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These authors contributed equally to this work.

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Ahn, JH., Jeong, YS., Lee, T.G. et al. Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry. BioChip J 6, 34–40 (2012). https://doi.org/10.1007/s13206-012-6105-8

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  • DOI: https://doi.org/10.1007/s13206-012-6105-8

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