Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry


A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL−1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.

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  1. 1.

    Cole, R.J. & Cox, R.H. Handbook of toxic fungal metabolites. Acedemic Press, New York (1981).

    Google Scholar 

  2. 2.

    Bennett, J.W. & Klich, M. Mycotoxins. Clin. Microbial. Rev. 16, 497–516 (2003).

    Article  CAS  Google Scholar 

  3. 3.

    Eaton, D.L. & Groopman, J.D. The Toxicology of Aflatoxins. Academic Press, New York (1994).

    Google Scholar 

  4. 4.

    Hoeltz, M., Welke, J.E., Noll, I.B. & Dottori, H.A. Photometric procedure for quantitative analysis of aflatoxin B1 in peanuts by thin-layer chromatography using charge coupled device detector. Quimica Nova 33, 43–47 (2010).

    Article  CAS  Google Scholar 

  5. 5.

    Buttinger, G. Aflatoxin measurements: how HPLC methods have evolved over the last 20 years? Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 27, 1266–1272 (2010).

    CAS  Google Scholar 

  6. 6.

    Goda, Y., Akiyama, H., Otsuki, T., Fujii, A. & Toyoda, M. Application and improvement of aflatoxin analysis in foods using a multifunctional column and HPLC. J. Food Hyg. Soc. Jap. 42, 56–62 (2001).

    Article  CAS  Google Scholar 

  7. 7.

    Lupo, A., Roebuck, C., Dutcher, M., Kennedy, J. & Abouzied, M. Validation study of a rapid ELISA for detection of aflatoxin in corn. J. AOAC Int. 93, 587–599 (2010).

    CAS  Google Scholar 

  8. 8.

    Radoi, A., Targa, M., Prieto-Simon, B. & Marty, J.L. Enzyme-Linked Immunosorbent Assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M-1 detection. Talanta 77, 138–143 (2008).

    Article  CAS  Google Scholar 

  9. 9.

    Kolosova, A.Y., Shim, W.B., Yang, Z.Y., Eremin, S.A. & Chung, D.H. Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B-1. Stabilization of ELISA kit components and application to grain samples. Anal. Bioanal. Chem. 384, 286–294 (2006).

    Article  CAS  Google Scholar 

  10. 10.

    Catharino, R.R. et al. Aflatoxin screening by MALDITOF mass spectrometry. Anal. Chem. 77, 8155–8157 (2005).

    Article  CAS  Google Scholar 

  11. 11.

    Belu, A.M., Graham, D.J. & Castner, D.G. Time-of-flight secondary ion mass spectrometry: techniques and applications for the characterization of biomaterial surfaces. Biomaterials 24, 3635–3653 (2003).

    Article  CAS  Google Scholar 

  12. 12.

    Pacholski, M.L. & Winograd, N. Imaging with mass spectrometry. Chem. Rev. 99, 2977–3005 (1999).

    Article  CAS  Google Scholar 

  13. 13.

    Reich, F. & Lindley, P.M. Applications of Time-of-Flight Secondary-Ion Mass-Spectrometry (Tof-Sims) to Organic-Surface Analysis. Surf. Biomater. Symposium 92–97 (1993).

  14. 14.

    Hagenhoff, B. High resolution surface analysis by TOF-SIMS. Microchim. Acta 132, 259–271 (2000).

    CAS  Google Scholar 

  15. 15.

    Wagner, M.S., Horbett, T.A. & Castner, D.G. Characterization of the structure of binary and ternary adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling. Langmuir 19, 1708–1715 (2003).

    Article  CAS  Google Scholar 

  16. 16.

    Kim, Y.P. et al. Quantitative analysis of surface-immobilized protein by TOF-SIMS: Effects of protein orientation and trehalose additive. Anal. Chem. 79, 1377–1385 (2007).

    Article  CAS  Google Scholar 

  17. 17.

    Kim, Y.P. et al. Quantitative ToF-SIMS study of surface-immobilized streptavidin. Appl. Surf. Sci. 252, 6801–6804 (2006).

    Article  CAS  Google Scholar 

  18. 18.

    Min, H., Yu, H., Son, M., Moon, D.W. & Lee, T.G. Quantitative analysis of the surface composition of mixed self-assembled monolayers using ToF-SIMS and FT-IR analyses. Surf. Interface Anal. 43, 397–401 (2011).

    Article  CAS  Google Scholar 

  19. 19.

    Abel, M.L. & Watts, J.F. Examination of the interface of a model adhesive joint by surface analysis: a study by XPS and ToF-SIMS. Surf. Interface Anal. 41, 508–516 (2009).

    Article  CAS  Google Scholar 

  20. 20.

    Tanaka, H., Takino, M., Sugita-Konishi, Y. & Tanaka, T. Development of a liquid chromatography/time-offlight mass spectrometric method for the simultaneous determination of trichothecenes, zearalenone and aflatoxins in foodstuffs. Rapid Commun. Mass Spectrom. 20, 1422–1428 (2006).

    Article  CAS  Google Scholar 

  21. 21.

    Owino, J.H.O. et al. Modelling of the impedimetric responses of an aflatoxin B-1 immunosensor prepared on an electrosynthetic polyaniline platform. Anal. Bioanal. Chem. 388, 1069–1074 (2007).

    Article  CAS  Google Scholar 

  22. 22.

    Saha, D., Acharya, D., Roy, D. & Dhar, T.K. Filtration-based tyramide amplification technique-a new simple approach for rapid detection of aflatoxin B-1. Anal. Bioanal. Chem. 387, 1121–1130 (2007).

    Article  CAS  Google Scholar 

  23. 23.

    Garcia-Villanova, R.J., Cordon, C., Paramas, A.M.G., Aparicio, P. & Rosales, M.E.G. Simultaneous immunoaffinity column cleanup and HPLC analysis of aflatoxins and ochratoxin A in Spanish bee pollen. J. Agric. Food Chem. 52, 7235–7239 (2004).

    Article  CAS  Google Scholar 

  24. 24.

    Sapsford, K.E. et al. Indirect competitive immunoassay for detection of aflatoxin B-1 in corn and nut products using the array biosensor. Biosens. Bioelectron. 21, 2298–2305 (2006).

    Article  CAS  Google Scholar 

  25. 25.

    Goryacheva, I.Y., Karagusheva, M.A., Van Peteghem, C., Sibanda, L. & De Saeger, S. Immunoaffinity preconcentration combined with on-column visual detection as a tool for rapid aflatoxin M1 screening in milk. Food Control 20, 802–806 (2009).

    Article  CAS  Google Scholar 

  26. 26.

    Kim, Y.P. et al. Gold nanoparticle-enhanced secondary ion mass spectrometry imaging of peptides on self-assembled monolayers. Anal. Chem. 78, 1913–1920 (2006).

    Article  CAS  Google Scholar 

  27. 27.

    Kim, Y.P. et al. Protein kinase assay on peptide-conjugated gold nanoparticles by using secondary-ion mass spectrometric imaging. Angew. Chem. Int. Ed. 46, 6816–6819 (2007).

    Article  CAS  Google Scholar 

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Correspondence to Young-Pil Kim or Hak-Sung Kim.

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These authors contributed equally to this work.

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Ahn, JH., Jeong, YS., Lee, T.G. et al. Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry. BioChip J 6, 34–40 (2012).

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  • Aflatoxin
  • Quantification
  • Multiplexed analysis