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Detection of typical indigenous gut bacteria related to kanpyo Lagenaria siceraria var. hispida powder in murine caecum and human faecal cultures

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Abstract

Kanpyo (KP) is an edible dried product produced by peeling the fruit of the gourd Lagenaria siceraria var. hispida; it is used in the traditional Japanese cuisine. The health functionality of KP due to its rich dietary fibre is expected to include a possible combined effect of KP-responsive indigenous gut bacteria (KP-RIB). However, its effect on the gut microbiota is unclear. To determine the effects of the KP on the gut microbiota and their host, Institute of Cancer Research mice were fed a high-sucrose diet containing no fibre (NF) or 5% (w/w) KP for 14 days, and their caecal microbiota was analysed by 16S rRNA (V4) amplicon sequencing. Higher faecal frequency and weight and lower spleen weight and spleen tumour necrosis factor-α levels were observed in KP-fed mice than in NF-fed mice (p < 0.05). KP increased and decreased the abundance of short-chain fatty acid producer Lachnospiraceae and obesity-inflammation related Allobaculum species, respectively. In the case of human faecal cultures, stool samples from five healthy volunteers were inoculated and incubated at 37 °C for 24 h anaerobically; 3.2% (w/v) KP suppressed putrefactive compounds (indole, phenol, and ammonia). KP increased butyrate-producer Faecalibacterium, acetate/lactate-producer Bifidobacterium, and Lachnospira. Furthermore, KP cultures showed high antioxidant and RAW264.7 macrophage cell activation capacities. These results suggest that KP-RIB and KP intake may synergistically affect host health. However, further studies are required to clarify the synergistic effects of KP and KP-RIB.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan

    Mahiro Yamamoto, Hikaru Ogura, Takashi Kuda, Yumeng Xia, Ayaka Nakamura & Hajime Takahashi

  2. AHJIKAN Co., Ltd., 7-3-9, Shoko Center, Nishiku, Hiroshima-City, Hiroshima, 733-8677, Japan

    Junji Inoue & Shu Takayanagi

Authors
  1. Mahiro Yamamoto
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  2. Hikaru Ogura
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  4. Yumeng Xia
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  6. Hajime Takahashi
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Contributions

MY and HO: conceptualisation, methodology, validation, formal analysis, investigation, resources, data curation, writing—original draft, and visualisation. TK: conceptualisation, methodology, validation, formal analysis, resources, data curation, writing—review and editing, visualisation, supervision, and project administration. YX and AN: conceptualisation, methodology, validation, formal analysis, investigation, resources, data curation, writing—original draft, and visualisation. HT, JI, and ST: conceptualisation, methodology, and supervision.

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Correspondence to Takashi Kuda.

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The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.

Ethical approval

This study was approved by the Animal Experiment Committee of the Tokyo University of Marine Science and Technology (Approval No. R3-1), and the Committee for Research Involving Human Subjects of the Tokyo University of Marine Science and Technology approved the study protocol (Approval No. R03-001).

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Yamamoto, M., Ogura, H., Kuda, T. et al. Detection of typical indigenous gut bacteria related to kanpyo Lagenaria siceraria var. hispida powder in murine caecum and human faecal cultures. 3 Biotech 14, 118 (2024). https://doi.org/10.1007/s13205-024-03960-5

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