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Characterization of Sclerotium rolfsii causing foot rot: a severe threat of betel vine cultivation in Bangladesh

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Abstract

The development of the foot rot disease caused by the fungus Sclerotium rolfsii is one of the primary variables endangering betel vine production in Bangladesh. Consequently, with the ultimate objective of finding efficient preventive and control strategies for this infamous phytopathogen, the current study was undertaken for comprehensive population structure analysis, exploration of physiological features and incidence patterns of pathogenic S. rolfsii isolates. We discovered 22 S. rolfsii isolates from nine northern districts of Bangladesh. Mohanpur (51.90%), Bagmara (54.09%), and Durgapur (49.45%) upazilas in the Rajshahi district had the more severe occurrences of foot rot disease, while Chapainawabganj (18.89%) had the least number of cases. The isolates differed substantially in terms of morphology and growth rate. By employing the UPGMA algorithm to analyze the combined morphological data from 22 S. rolfsii isolates, these isolates were divided into six different groups with a 62% similarity level. Somatic incompatibility was also found in some isolates. The RAPD-4 primer confirmed 100% polymorphism among these isolates, and these genetic variations were further validated by molecular analysis. The results of the morphological and molecular analysis revealed that there was significant variation among the S. rolfsii isolates. Finally, a comprehensive characterization of S. rolfsii would allow for a suitable management strategy for betel vine’s deadly foot rot disease.

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The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

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Acknowledgements

The first author was awarded the NST fellowship from the Ministry of Science and Technology, Bangladesh. The authors would like to thank Professor Robert L. Wick, University of Massachusetts, USA, for his critical review and valuable comments. The authors are highly grateful to betel vine farmers for their cooperation during the study.

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Authors and Affiliations

  1. Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Nargis Tanjila & Ferdousi Begum

  2. Plant Pathology Division, Bangladesh Wheat and Maize Research Institute (BWMRI), Nashipur, Dinajpur, 5200, Bangladesh

    Shaikhul Islam

  3. Plant Pathology Division, Agricultural Research Institute (BARI), Joydebpur, Gazipur, 1701, Bangladesh

    Md. Shamim Akhter

  4. Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Md. Monzur Hossain

  5. Mycology and Plant Pathology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Mohammad Shahidul Alam

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  1. Nargis Tanjila
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Correspondence to Md. Shamim Akhter or Ferdousi Begum.

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Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (JPG 2671 KB) Supplementary Fig. 1 Mycelial growth patterns of the isolated S. rolfsii isolates

13205_2023_3890_MOESM2_ESM.docx

Supplementary file2 (DOCX 16 KB) Supplementary Table 1. Morphological characteristics used for the description of S. rolfsii Sacc and their attributes (Banakar et al. 2017) Supplementary Table 2. Name of RAPD primers and their sequences.

Supplementary file3 (XLSX 11 KB) Supplementary Table 3 Data used for correlation analysis

13205_2023_3890_MOESM4_ESM.xlsx

Supplementary file4 (XLSX 11 KB) Supplementary Table 4 The results of the correlation analysis that was used for preparing the correlation plot

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Tanjila, N., Islam, S., Akhter, M.S. et al. Characterization of Sclerotium rolfsii causing foot rot: a severe threat of betel vine cultivation in Bangladesh. 3 Biotech 14, 58 (2024). https://doi.org/10.1007/s13205-023-03890-8

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