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Isolation and characterization of rhizobacteria from lentil for arsenic resistance and plant growth promotion

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Abstract

Low-cost microbial remediation strategies serve as a viable and potent weapon for curbing the arsenic menace. In the present study, two arsenic-resistant bacteria were isolated from the contaminated lentil rhizosphere in Gangetic plain of eastern India. LAR-21 (Burkholderia cepacia, MW356875) and LAR-25 (Burkholderia cenocepacia, MW356894) could remove 87.6% and 85.9% of arsenite (10 mM) from the liquid culture medium in laboratory condition. They were highly resistant to arsenate and arsenite and also had a high arsenite oxidase activity. LAR-21 showed the highest level of minimum inhibitory concentration value of 390 mM for arsenate and 31 mM for arsenite. The same strain was found to show highest arsenite oxidase activity, i.e., 5.2 nM min−1 mg−1of protein. These two strains further possess potential plant growth-promoting characteristics like indole acetic acid production (5–15 mM IAA mL−1), 1-aminocyclopropane-1-carboxylate deaminase (8–21 nM α-keto butyrate mg protein−1 h−1), nitrogenase activity (3–8.99 nM ethylene mg cell protein−1 h−1), siderophore production (17–22.1 µM deferoxamine mesylate mL−1), phosphate solubilization (261–453 µg mL−1) under arsenic stress condition. The plant growth promotion of the strains was further validated by pot study of lentil by assessing their agronomic and growth-related traits, and potential to recover from arsenic stress (17.2–21.2% arsenic reduction in root and shoot, 16–19.2% in leaf and pod, and 15–23% reduction in seeds). The LAR-21 strain, thus, emerged as the most suited candidate for bioremediation and plant (lentil) growth promotion in arsenic polluted environment.

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Acknowledgements

The authors are grateful to the ICAR-Niche area of Excellence-Arsenic Research Laboratory, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, Nadia as well as Department of Genetics & Plant Breeding and Department of Agronomy, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur, Nadia, West Bengal, India for providing technical and all other necessary assistance during the study.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Aritri Laha and Sudip Sengupta have contributed equally to the development of the manuscript.

Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, Swami Vivekananda University, Barrackpore, Kolkata, West Bengal, 700121, India

    Aritri Laha

  2. Department of Genetics and Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Aritri Laha & Somnath Bhattacharyya

  3. Department of Agricultural Chemistry and Soil Science, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Sudip Sengupta & Kallol Bhattacharyya

  4. School of Agriculture, Swami Vivekananda University, Barrackpore, Kolkata, West Bengal, 700121, India

    Sudip Sengupta

  5. Department of Botany, West Bengal State University, Barasat, Kolkata, West Bengal, 700126, India

    Sanjoy GuhaRoy

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  1. Aritri Laha
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Contributions

A.L.: Methodology, Investigation, Data curation, Software and Writing-Original draft preparation; S.S.: Data curation, Visualization, Software, Writing-Reviewing and Editing; S.B.: Conceptualization, Methodology, Supervision; K.B.: Conceptualization, Methodology, Validation, Formal reviewing; S.G.: Conceptualization, Methodology, and Supervision.

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Correspondence to Aritri Laha.

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Laha, A., Sengupta, S., Bhattacharyya, S. et al. Isolation and characterization of rhizobacteria from lentil for arsenic resistance and plant growth promotion. 3 Biotech 14, 30 (2024). https://doi.org/10.1007/s13205-023-03873-9

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