Abstract
The plant–parasitic Root Knot Nematodes (Meloidogyne spp.,) play a pivotal role to devastate vegetable crops across the globe. Considering the significance of plant–microbe interaction in the suppression of Root Knot Nematode, we investigated the diversity of microbiome associated with bioagents-treated and nematode-infected rhizosphere soil samples through metagenomics approach. The wide variety of organisms spread across different ecosystems showed the highest average abundance within each taxonomic level. In the rhizosphere, Proteobacteria, Firmicutes, and Actinobacteria were the dominant bacterial taxa, while Ascomycota, Basidiomycota, and Mucoromycota were prevalent among the fungal taxa. Regardless of the specific treatments, bacterial genera like Bacillus, Sphingomonas, and Pseudomonas were consistently found in high abundance. Shannon diversity index vividly ensured that, bacterial communities were maximum in B. velezensis VB7-treated soil (1.4–2.4), followed by Root Knot Nematode-associated soils (1.3–2.2), whereas richness was higher with Trichoderma konigiopsis TK drenched soils (1.3–2.0). The predominant occurrence of fungal genera such as Aspergillus Epicoccum, Choanephora, Alternaria and Thanatephorus habituate rhizosphere soils. Shannon index expressed the abundant richness of fungal species in treated samples (1.04–0.90). Further, refraction and species diversity curve also depicted a significant increase with maximum diversity of fungal species in B. velezensis VB7-treated soil than T. koningiopsis and nematode-infested soil. In field trial, bioagents-treated tomato plant (60% reduction of Meloidogyne incognita infection) had reduced gall index along with enhanced plant growth and increased fruit yield in comparison with the untreated plant. Hence, B. velezensis VB7 and T. koingiopsis can be well explored as an antinemic bioagents against RKN.
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Acknowledgements
This work was supported by the Agricultural Bioinformatics – BIC facility available at the Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. The authors acknowledge the Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India for providing facilities. The authors extends their appreciation to Researchers Supporting Project number (RSPD2023R979), King Saud University, Riyadh, Saudi Arabia, for financial assistance.
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SN conceptualized the research and was associated with technically guiding and executing the research. KV prepared the manuscript and carried out the data analysis. SN and NS edited the manuscript. PJ provided the resources for the experiment. GP performed the metagenomic analysis. NS, KP and MA have supported the analysis of data.
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Vinothini, K., Nakkeeran, S., Saranya, N. et al. Metagenomic profiling of tomato rhizosphere delineates the diverse nature of uncultured microbes as influenced by Bacillus velezensis VB7 and Trichoderma koningiopsis TK towards the suppression of root-knot nematode under field conditions. 3 Biotech 14, 2 (2024). https://doi.org/10.1007/s13205-023-03851-1
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DOI: https://doi.org/10.1007/s13205-023-03851-1