Abstract
In this study, through analysis of the genome of Eleutherococcus senticosus (ES). 228 AP2/ERF genes were identified and classified into 5 groups AP2 (47 genes), ERF (108 genes), RAV (6 genes), DREB (64 genes), and soloist (3 genes). According to the AP2/ERF classification of Arabidopsis thaliana, the ES AP2/ERF proteins were subdivided into 15 groups. The gene structure and motifs of each group of AP2/ERF in ES were highly similar, which confirmed the conservation of AP2/ERF genes. The ES AP2/ERF genes were unevenly distributed on chromosomes, and a total of four pairs of tandem repeats, and 84 co-linear gene pairs were found, so the AP2/ERF genes expanded in a fragment replication manner, and dominated by pure selection during evolution. By analyzing the transcriptome data of ES under different drought stress conditions, 87 AP2/ERF genes with differential expression were obtained, of which 10 genes with highly significant differences were further analyzed and screened for qRT-PCR validation. To the best of our knowledge, this is the first report on the AP2/ERF gene of Eleutherococcus senticosus, and the bioinformatics analysis and experimental validation provided valuable information about them, which is of great significance for further research on the molecular mechanisms of ES in response to drought stress.
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Data availability
The data that support the findings of this study are available from the NCBI database (Accession: SRR19962743-SRR19962757) but restrictions apply to the availability of these data, which were used under license for the current study, and so are not publicly available. Data are however available from the authors upon reasonable request and with the permission of Zhaobin Xing (xingzb@ncst.edu.cn).
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Funding
This work was supported by Natural Science Foundation of Hebei Province (H2020209302) and the Innovation and Entrepreneurship Training Program for College Students of North China University of Science and Technology (X2022243).
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The first draft of the manuscript was written by M-YJ, and all authors commented on previous versions of the manuscript. Material preparation, data collection, and analysis were performed by JZ, and experiments were performed by W-wC and XS. Z-bX and Y-hL contributed to the study conception and design. All authors read and approved the final manuscript.
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Jiao, MY., Zhang, J., Cheng, Ww. et al. Identification of the AP2/ERF transcription factor family of Eleutherococcus senticosus and its expression correlation with drought stress. 3 Biotech 13, 259 (2023). https://doi.org/10.1007/s13205-023-03678-w
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DOI: https://doi.org/10.1007/s13205-023-03678-w