Abstract
In this study, we described the carbapenem blaNDM-5-carrying extensive drug-resistant (XDR) K. pneumoniae ST437 from an urban river water Kathajodi in Odisha, India. The presence of carbapenem and co-occurrence of other resistance determinants (blaNDM-5, blaCTX-M, blaSHV, and blaTEM), virulence factors (fimH, mrkD, entB, irp-1, and ybtS), and capsular serotype (K54) represent its pathogenic potential. The insertion sequence ISAba125 and the bleomycin resistance gene bleMBL at upstream and downstream, respectively, could play a significant role in the horizontal transmission of the blaNDM-5. Its biofilm formation ability contributes toward environmental protection and its survivability. MLST analysis assigned the isolate to ST437 and clonal lineage to ST11 (CC11) with a single locus variant. The ST437 K. pneumoniae, a global epidemic clone, has been reported in North America, Europe, and Asia. This work contributes in understanding of the mechanisms behind the spread of blaNDM-5 K. pneumoniae ST437 and demands extensive molecular surveillance of river and nearby hospitals for better community health.
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Data availability
The data that support the finding of this study are available in the supplementary material of this article.
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Acknowledgements
The authors are grateful to Prof (Dr.) S.C. Si, Dean, Centre for Biotechnology and Prof (Dr.) M.R. Nayak, President, Siksha ‘O’ Anusandhan University, for providing infrastructure and encouragement throughout.
Funding
This research work was supported by the DST-INSPIRE [DST/INSPIRE/03/2018/000860], New Delhi, India.
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The first draft of manuscript was written by SS. Conceptualization of the study and review of the manuscript were performed by SS, ES, RKS. Sample collection and laboratory: SS, DUB, RKS; interpretation of data and data analysis: SS, ES, SD; supervision: ES. All authors read and approved the final manuscript.
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Sahoo, S., Sahoo, R.K., Dixit, S. et al. NDM-5-carrying Klebsiella pneumoniae ST437 belonging to high-risk clonal complex (CC11) from an urban river in eastern India. 3 Biotech 13, 139 (2023). https://doi.org/10.1007/s13205-023-03556-5
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DOI: https://doi.org/10.1007/s13205-023-03556-5