Abstract
Cercospora sesami is a plant pathogen that causes leaf spot disease in sesame plants worldwide. In this study, genome sequence assembly of C. sesami isolate Cers 52–10 (MCC 9069) was generated using native paired-end and mate-pair DNA sequencing based on the Illumina HiSeq 2500 platform. The genome assembly of C. sesami is 34.3 Mb in size with an N50 of 26,222 bp and an average GC content of 53.02%. A total number of 10,872 genes were predicted in this study, out of which 9,712 genes were functionally annotated. Genes assigned to carbohydrate-active enzyme classes were also identified during the study. A total of 80 putative effector candidates were predicted and functionally annotated. The C. sesami genome sequence is available at DDBJ/ENA/GenBank, and other associated information is submitted to Mendeley's data.
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The authors state that all the necessary data to confirm the conclusions presented in the article are entirely included within the article, and the supplementary data to this article have been deposited to Mendeley’s data with DOI number https://doi.org/10.17632/26m5grnb88.1.
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Acknowledgements
Shagun Sinha is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the Senior Research Fellowship (SRF) and the Department of Biotechnology (DBT) and the British Council for Newton-Bhabha PhD placement fellowship 2019-2020. All authors are thankful to the Head and Coordinator, Center of Advanced Studies (CAS) in Botany, Banaras Hindu University (BHU), Varanasi, India and the Head, Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, BHU for financial and infrastructural support.
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Council of Scientific and Industrial Research, India, 09/013(0824)/2018-EMR-I, Shagun Sinha.
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RC, RNK and SN planned the whole-genome sequencing study. RC and SS contributed to the identification, isolation and characterization of the sequenced pathogen. SS and SN performed DNA extraction, gene annotation and phylogenetic analysis. SS and DKG helped in bioinformatics analysis. SS and SN drafted the manuscript. RNK was involved in adding critical inputs to the manuscript. All authors have read and contributed to the final manuscript.
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Accession Numbers: The Cercospora sesami genomic data have been deposited as accession JAASLG000000000 in DDBJ/ENA/GenBank. The version described in this paper is version JAASLG010000000. The genome raw sequencing data and the reported assembly are associated with NCBI BioProject: PRJNA613165 and BioSample: SAMN14395398 within GenBank. The SRA accession of Cercospora sesami is SRX7967432.
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Sinha, S., Navathe, S., Singh, S. et al. Genome sequencing and annotation of Cercospora sesami, a fungal pathogen causing leaf spot to Sesamum indicum. 3 Biotech 13, 55 (2023). https://doi.org/10.1007/s13205-023-03468-4
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DOI: https://doi.org/10.1007/s13205-023-03468-4