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Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice

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Abstract

This study aimed to examine the effect of Debaryomyces hansenii CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two D. hansenii (Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with Escherichia coli. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (p < 0.05) upon 7 days of D. hansenii intake at a concentration of 10ˆ8 CFU before and after bacterial challenge. Moreover, oral D. hansenii in mice upregulated (p < 0.05) gene expression of pro-inflammatory cytokines (INF-γ, IL-6 and IL-1β) before or after E. coli challenge on day 7 but downregulated (p < 0.05) on day 15. Furthermore, total serum IgG and IgA titers were higher (p < 0.05) in Dh 10ˆ8 CFU at days 7 and 15, and only at day 7, respectively, than that in the other dose and control groups. Finally, D. hansenii was detected in the gut of mice that received the treatments, suggesting that yeast survived gastrointestinal transit. Altogether, a short period (7 days) of D. hansenii CBS 8339 oral delivery improved immune innate response on mice.

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Data availability statement

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful to Margarito Rodríguez (Plant Biotechnology Immunology and Vaccinology Lab.), Guadalupe Sanchez (Bioterium) and Orlando Lugo (Oxidative Stress Lab.) from CIBNOR for technical assistance; and Diana Fischer for English edition. Miriam Angulo thanks CONACYT for postdoctoral fellowship Grant number 935561.

Funding

This research was financially supported by CONACYT (INFR-2014-01/225924 and PDCPN2014-01/248033).

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  1. Immunology and Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste, S.C., Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, BCS, Mexico

    Miriam Angulo, Abel Ramos, Martha Reyes-Becerril, Kevyn Guerra, Elizabeth Monreal-Escalante & Carlos Angulo

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  1. Miriam Angulo
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Contributions

MA and CA conceived the study. MA, AR-V, MR-B, KG, and EM-E performed the experiments. MA and CA wrote the initial draft of the manuscript. CA involved in project administration. CA involved in funding acquisition. All the authors commented on working versions of the manuscript and agreed on the final version of the manuscript.

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Correspondence to Carlos Angulo.

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Angulo, M., Ramos, A., Reyes-Becerril, M. et al. Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice. 3 Biotech 13, 28 (2023). https://doi.org/10.1007/s13205-022-03442-6

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