Abstract
Phosphorus (P) availability is determinant for crop productivity and, despite the sufficient amount of this nutrient in arable land, most of it remains insoluble, leading to the need of high fertilizer input. To cope with P scarcity forecasts and also for cropping costs alleviation, genotypes better adapted to promote P solubilization and absorption are required, especially for perennial crops. Coffee is one of these important perennial crops cultivated in soils with low P availability, and thus we aimed to study adaptive strategies to coffee genotypes in acquire phosphorus. In this study, we focused on rhizosphere phosphatase activity, a major characteristic related to P solubilization from organic pools. To explore the genetic basis of this characteristic, we firstly identified 29 Purple Acid Phosphatases (PAP) genes in C. canephora genome and selected five candidates with higher potential to encode secreted PAPs. We found that C. arabica genotypes have diverse profiles of rhizosphere phosphatase activity, as well as microbial biomass carbon, which we measured to explore the impact of the plant on microbiota and also to discriminate the phosphatase activity origin (plant or microorganism-derived). We selected two C. arabica cultivars with contrasting phosphatase activity and found that one PAP gene has a correlated gene expression profile with this characteristic. This work explores coffee adaptative responses to P starvation conditions, and the information provided can further contribute to breeding programs aiming better adapted genotypes for sustainable agriculture in face of current challenges.
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Acknowledgments
To FAPEMIG, CNPq and Consórcio Pesquisa Café (Embrapa/Café) for financial support. To CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for granting the scholarship. “This work was carried out with the support of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Financing Code 001”.
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DV, RP and TC carried out the experiments, analyzed the data and wrote the manuscript. LP, MAC and GC designed the research and edited the manuscript. JS designed the research, carried out the experiments and analyzed the data.
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Vilela, D.J.M., Pinto, R.T., Cardoso, T.B. et al. Purple acid phosphatases in coffee—genome-wide identification and the transcriptional profile of selected members in roots upon Pi starvation. 3 Biotech 12, 335 (2022). https://doi.org/10.1007/s13205-022-03399-6
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DOI: https://doi.org/10.1007/s13205-022-03399-6