Abstract
A novel strain, designated BJN0001, was isolated from the cellar mud of Chinese strong-flavor baijiu. The complete genome of strain BJN0001 was 2,688,791 bp and annotated with 2610 genes. Whole-genome similarity metrics such as average nucleotide identity (ANI) of BJN0001 with reference genomes reveals clear species boundaries of < 95% ANI value for species. The DNA–DNA hybridization (DDH) values of BJN0001 with the type species were all lower than 70% DDH value for species. Based on these results, the strain BJN0001 was considered a potentially new species of the genus Clostridium. Meanwhile, the fermentation characteristics indicated that the strain had the capability to convert glucose to ethanol, acetic acid and butyric acid, which could provide basic data for revealing its function in baijiu fermentation.
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Data availability
The genome sequence was submitted to NCBI under the GenBank accession number CP094971. The BioProject accession number was PRJNA821263, and the BioSample accession number was SAMN27064741.
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Acknowledgements
This work was supported by National Natural Science Foundation of China (No. 32072165, 31830069, 31801467), Beijing Municipal Natural Science Foundation & Beijing Municipal Education Commission (No. KM202110011003, KZ202010011018), School Level Cultivation Foundation of Beijing Technology and Business University for Distinguished and Excellent Young Scholars (No. BTBUYP2021), and Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry (2021JJ006).
Funding
National Natural Science Foundation of China, 32072165, Youqiang Xu, 31830069, Xiuting Li, 31801467, Youqiang Xu, Beijing Municipal Natural Science Foundation; Beijing Municipal Education Commission, KM202110011003, Youqiang Xu, KZ202010011018, Xiuting Li, School Level Cultivation Foundation of Beijing Technology and Business University for Distinguished and Excellent Young Scholars, BTBUYP2021, Youqiang Xu, Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, 2021JJ006, Youqiang Xu.
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YX: methodology, investigation, data curation, writing—original draft, funding acquisition, and supervision. MW: methodology, investigation, and data curation. JN: data curation and methodology. HH: methodology and investigation. ZN: data curation. ZF: data curation. CZ: data curation. ZZ: data curation. HL: data curation. XL: resources, writing—review and editing, funding acquisition, and supervision. BS: resources, writing—review and editing, funding acquisition, and supervision.
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Xu, Y., Wu, M., Niu, J. et al. Clostridium btbubcensis BJN0001, a potentially new species isolated from the cellar mud of Chinese strong-flavor baijiu, produces ethanol, acetic acid and butyric acid from glucose. 3 Biotech 12, 203 (2022). https://doi.org/10.1007/s13205-022-03271-7
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DOI: https://doi.org/10.1007/s13205-022-03271-7