Abstract
A strictly anaerobic, gram-positive bacterium, designated as WLY-B-L2T, was isolated from pit mud of a fermentation pit located at Wuliangye 501# baijiu workshop in Yibin (Sichuan province, PR China). The strain was strictly anaerobic, Gram-positive with straight or somewhat rod shaped cells which were 0.5–0.7 μm wide and 1.7–3.1 μm long, arranged singly or in pairs. The strain can utilize D-galacturonic acid, methyl pyruvate, L-lactamine, L-alanyl − L-glutamine, L-alanyl − L-histidine, glycerol, pyruvate, L-alanyl − L-threonine, L-methionine, L-phenylalanine, L-valine + L-aspartic acid, L-serine, L-valine, and thymidine as carbon sources. Major cellular fatty acids are C16: 0 (24.6%), anteiso-C15: 0 (16.5%), and iso-C15: 0 (14.1%). Based on 16S rRNA gene sequence, WLY-B-L2T is most closely related to Clostridium luticellarii FW431T; they exhibit 97.42% 16S rRNA similarity. Additionally, the digital DNA-DNA hybridization (dDDH) value between them is 28.10%. WLY-B-L2T has a G + C content of 34.16 mol%. Based on the evidence presented here, WLY-B-L2T (CICC 25133T = JCM 35127T) is proposed as the type strain of a novel species, designated as Clostridium aromativorans sp. nov, that could produce butyric acid and volatile flavor components, such as ethyl valerate, ethyl acetate and 2-pentanone.
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Abbreviations
- SATB:
-
Strong aroma-type baijiu
- MIDI:
-
Sherlock microbial identification system
- HPLC:
-
High Performance liquid chromatography
- dDDH:
-
Digital DNA–DNA hybridization
- PCR:
-
Polymerase chain reaction
- GGDC:
-
Genome-to genome distance calculation
- RCM:
-
Reinforced clostridium medium
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Acknowledgements
The authors thank Dr. Chaolan Liu (Faculty of Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, School of Pharmacy, Chengdu University) for the help to the identification of WLY-B-L2T, Prof. Aharon Oren (Department of Plant and Environmental Sciences, the Alexander Silberman Institute of Life Sciences, the Hebrew University of Jerusalem) and Dr. Lei Zhai (China Center of Industrial Culture Collection, CICC) for the help to the nomenclature of WLY-B-L2T.
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QL: Writing-original draft (lead); JZ: Concept design, revise, and reviewing (lead); Investigation (lead). DZ: Funding acquisition (equal); Resources (equal). DL: Resources (equal).
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Luo, Q., Zheng, J., Zhao, D. et al. Clostridium aromativorans sp. nov., isolated from pit mud used for producing Wuliangye baijiu. Antonie van Leeuwenhoek 116, 739–748 (2023). https://doi.org/10.1007/s10482-023-01841-0
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DOI: https://doi.org/10.1007/s10482-023-01841-0