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Silver nanoparticles for biolistic transformation in Nicotiana tabacum L.

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Abstract

The present study reports the use of silver nanoparticles as a gene carrier, substituting gold microcarrier for biolistic gene delivery in Nicotiana tabacum L. Efficiency of biolistic transformation using silver nanoparticles (100 nm) was compared with that of gold microcarriers (0.6 micron) under varying helium pressure (450 psi, 650 psi, 900 psi and 1100 psi) and target distance (6 cm and 9 cm). Among the different concentrations (0.01–100 mgL−1) of silver nanoparticles tried, 10 mgL−1 produced the highest number of transient GUS expression (30) with statistical significance. Helium pressure of 650 and target distance of 9 cm, and 900 psi pressure and 6 cm distance resulted in the highest GUS expression with gold microcarriers and silver nanoparticles, respectively. Transformation efficiency was significantly higher with silver nanoparticles than gold microparticles as carriers resulting in a reduction up to 37.5-fold on the cost of consumables. Regeneration efficiencies of tissues bombarded with gold microcarriers and silver nanoparticles were 62.5% and 70.83%, respectively.

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Acknowledgements

Research facilities provided by Kerala Agricultural University is gratefully acknowledged

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Authors and Affiliations

  1. Department of Plant Biotechnology, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram, Kerala, 695522, India

    Nitasana Rajkumari, Swapna Alex, K. B. Soni & A. G. Kiran

  2. Department of Agricultural Microbiology, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram, Kerala, 695522, India

    K. N. Anith

  3. Department of Plant Physiology, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram, Kerala, 695522, India

    M. M. Viji

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  1. Nitasana Rajkumari
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  2. Swapna Alex
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  3. K. B. Soni
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  4. K. N. Anith
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  5. M. M. Viji
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  6. A. G. Kiran
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Correspondence to Swapna Alex.

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Rajkumari, N., Alex, S., Soni, K.B. et al. Silver nanoparticles for biolistic transformation in Nicotiana tabacum L.. 3 Biotech 11, 497 (2021). https://doi.org/10.1007/s13205-021-03043-9

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