Abstract
In this study, the draft genome of the recently isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193, which can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about POA-rich lipid production by Scheffersomyces segobiensis so far. The ITS region analysis indicated that strain DSM 27193 is closely related to Pichia segobiensis and Scheffersomyces stipitis. The size of the assembled draft genome of strain DSM 27193 is 14.8 Mb, containing 5477 encoded protein sequences with a G + C content of 40.83%. Among the annotated genes, two stearoyl-CoA desaturases encoded by ole1 and ole2 were identified which are potentially involved in POA accumulation. Further analysis of POA-rich lipid synthesis pathway genes in S. segobiensis DSM 27193 will provide additional insights for POA artificial synthesis through metabolic engineering.
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References
Bai Y, McCoy JG, Levin EJ, Sobrado P, Rajashankar KR, Fox BG, Zhou M (2015) X-ray structure of a mammalian stearoyl-CoA desaturase. Nature 524(7564):252–256. https://doi.org/10.1038/nature14549
Chen H, Gu Z, Zhang H, Wang M, Chen W, Lowther WT, Chen YQ (2013) Expression and purification of integral membrane fatty acid desaturases. PLoS ONE 8(3):e58139. https://doi.org/10.1371/journal.pone.0058139
Cho J-Y, Jeffries TW (1999) Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis. Appl Environ Microbiol 65(6):2363–2368. https://doi.org/10.1128/AEM.65.6.2363-2368.1999
Klinner U, Fluthgraf S, Freese S, Passoth V (2005) Aerobic induction of respiro-fermentative growth by decreasing oxygen tensions in the respiratory yeast Pichia stipitis. Appl Microbiol Biotechnol 67(2):247–253. https://doi.org/10.1007/s00253-004-1746-8
Kolouchová I, Sigler K, Schreiberová O, Masák J, Řezanka T (2015) New yeast-based approaches in production of palmitoleic acid. Bioresour Technol 192:726–734. https://doi.org/10.1016/j.biortech.2015.06.048
Lang I, Hodac L, Friedl T, Feussner I (2011) Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biol 11(1):1–16. https://doi.org/10.1186/1471-2229-11-124
Matsunaga T, Takeyama H, Miura Y, Yamazaki T, Furuya H, Sode K (1995) Screening of marine cyanobacteria for high palmitoleic acid production. FEMS Microbiol Lett 133(1–2):137–141. https://doi.org/10.1111/j.1574-6968.1995.tb07874.x
Osundeko O, Ansolia P, Gupta SK, Bag P, Bajhaiya AK (2019) Promises and challenges of growing microalgae in wastewater. In: Singh R, Kolok A, Bartelt-Hunt S (eds) Water conservation, recycling and reuse: issues and challenges. Springer, Singapore. https://doi.org/10.1007/978-981-13-3179-4_2
Papini M, Nookaew I, Uhlén M, Nielsen J (2012) Scheffersomyces stipitis: a comparative systems biology study with the Crabtree positive yeast Saccharomyces cerevisiae. Microb Cell Factories 11(1):1–16. https://doi.org/10.1186/1475-2859-11-136
Ratledge C (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochem Soc Trans 30(6):1047–1050. https://doi.org/10.1042/bst0301047
Schulze I, Hansen S, Großhans S, Rudszuck T, Ochsenreither K, Syldatk C, Neumann A (2014) Characterization of newly isolated oleaginous yeasts-Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis. AMB Express 4(1):1–11. https://doi.org/10.1186/s13568-014-0024-0
Shanmugam S, Sun C, Zeng X, Wu Y-R (2018) High-efficient production of biobutanol by a novel Clostridium sp. strain WST with uncontrolled pH strategy. Bioresour Technol 256:543–547. https://doi.org/10.1016/j.biortech.2018.02.077
Tehlivets O, Scheuringer K, Kohlwein SD (2007) Fatty acid synthesis and elongation in yeast. Biochim Biophys Acta Mol Cell Biol Lipids 1771(3):255–270. https://doi.org/10.1016/j.bbalip.2006.07.004
Wang H, Gao L, Chen L, Guo F, Liu T (2013) Integration process of biodiesel production from filamentous oleaginous microalgae Tribonema minus. Bioresour Technol 142:39–44. https://doi.org/10.1016/j.biortech.2013.05.058
Wang H, Ji B, Wang J, Guo F, Zhou W, Gao L, Liu TZ (2014) Growth and biochemical composition of filamentous microalgae Tribonema sp. as potential biofuel feedstock. Bioprocess Biosyst Eng 37(12):2607–2613. https://doi.org/10.1007/s00449-014-1238-x
Wu Y, Li R, Hildebrand DF (2012) Biosynthesis and metabolic engineering of palmitoleate production, an important contributor to human health and sustainable industry. Prog Lipid Res 51(4):340–349. https://doi.org/10.1016/j.plipres.2012.05.001
Yan W, Zhang S, Wu M, Zhang W, Zhou J, Dong W, Qian X, Jiang M, Xin F (2019) The draft genome sequence of Meyerozyma guilliermondii strain YLG18, a yeast capable of producing and tolerating high concentration of 2-phenylethanol. 3 Biotech 9(12):1–5. https://doi.org/10.1007/s13205-019-1975-2
Acknowledgements
This work was supported by the National Key R & D Program of China (2019YFA0905700, 2018YFA0902200), National Natural Science Foundation of China (22008115, 21978130), Jiangsu Province Natural Science Foundation for Youths (SBK2020044721), China Postdoctoral Science Foundation (2020M671467), and Project of State Key Laboratory of Materials-Oriented Chemical Engineering (ZK201801).
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Zhou, X., Xu, L., Zhou, D. et al. The draft genome sequence of Scheffersomyces segobiensis strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids. 3 Biotech 11, 488 (2021). https://doi.org/10.1007/s13205-021-03040-y
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DOI: https://doi.org/10.1007/s13205-021-03040-y